A kind of sensitized drug composition for treating ovarian cancer and its application
A technology of composition and application, applied in the direction of drug combination, active ingredient of hydroxyl compound, active ingredient of heterocyclic compound, etc., can solve problems such as poor prognosis, unsatisfactory effect, and low survival rate, so as to enhance the therapeutic effect and prevent drug resistance , reducing the effect of drug resistance
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Embodiment 1
[0026] Example 1 The sensitization effect of autophagy inhibitors on the treatment and prevention of ovarian cancer by resveratrol
[0027] 1.1 Main materials and reagents Human ovarian serous papillary cystadenocarcinoma SKOV3 cell line, provided by the Cancer Research Institute of the Third Affiliated Hospital of Harbin Medical University; Res was dissolved in dimethyl sulfoxide (DMSO) to prepare a 100 mmol / L original solution , stored in the dark at -20°C; RPMI-1640 medium (Gibco, USA); fetal bovine serum (Hangzhou Sijiqing Company); rabbit anti-human Beclin1, LC3-II IgG polyclonal antibody (Santa Company); CCK-8 reagent 3-MA, MDC dyes (Sigma, USA); Z-VAD-FMK 20 μM, stored at -20°C (Sigma, USA); Annexin V / PI apoptosis detection kit was purchased from Shanghai Union Branch Biological Co., Ltd.; 2×Taq Master Mix (Shanghai Laifeng Biotechnology Co., Ltd.); RNase Free water (Beijing Zhongshan Jinqiao Technology Co., Ltd.); M-MuLV Reverse Transcriptase (Beijing Zhongshan Jinqi...
Embodiment 2
[0037] Example 2 Mechanism research on the sensitization effect of autophagy inhibitors on the treatment and prevention of ovarian cancer by resveratrol
[0038] 1.1 SKOV3 cells were routinely cultured in RPMI-1640 medium containing 10% fetal bovine serum, 100 U / mL penicillin and 100 μg / mL streptomycin, at 37°C, 5% CO 2 Cultivate in an incubator, subculture once every 2-3 days, and take cells in the logarithmic growth phase for experiments.
[0039] 1.2 Cell grouping and drug treatment Experimental cells were divided into 4 groups: control group (Control), resveratrol group (Res), 3-MA group, 3-MA+Res group, Z-VAD-FMK+Res group. The control group was added with an equal volume of DMEM; the Res group was treated with Res with an experimental concentration of 25 μmol / L; Cells were treated with MA, and 25 μmol / L Res was added after 1 hour; cells in the Z-VAD-FMK+Res group were first treated with 20 μM Z-VAD-FMK, and 25 μmol / L Res was added after 30 minutes, and the cells in ea...
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