Unlock instant, AI-driven research and patent intelligence for your innovation.
Biological type beef tendering agent as well as preparation method and application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A bio-type, tenderizer technology, applied in the field of microorganisms, can solve problems such as difficulty in cost, and achieve the effects of improving tenderizing effect, improving activity, and improving activity
Inactive Publication Date: 2017-06-06
HUNAN AGRICULTURAL UNIV
View PDF1 Cites 2 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
There are many studies on post-mortem tenderization at home and abroad, especially enzymatic tenderization. At present, papain, ficin and bromelain are the most widely used and achieved results, which can degrade myofibrils and improve the tenderness of meat. However, due to the limitation of raw materials, this kind of protease has certain difficulties in practical application due to cost problems.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0025] A preparation method of beef bio-type tenderizer, comprising the following steps:
[0026] (1) Isolation of Bacillus amyloliquefaciens from Guizhou traditional tempeh and sweet wine koji. The strain is white, smooth and moist colony, slightly raised, with folds in the middle of the colony, which is a spore-forming Gram-positive bacillus, and a clearly visible transparent circle will be formed around it. It is filmy on slopes and has a white surface.
[0027] (2) The strain was purely cultured with an isolation medium, cultured in a biochemical medium box at 37°C for 24 hours, and then stored on a slant.
[0029] (3) Pick the strain of Bacillus amyloliquefaciens from the slant culture medium, inoculate it into the broth medium, culture it on a shaker at 220 r / min at 37°C for 12 hours, and activate it...
Embodiment 2
[0033] A preparation method of a beef biological tenderizer, wherein the fermentation medium formula is:
[0035] The remaining steps are the same as in Example 1, and the enzyme activity of the beef biological tenderizer obtained after cultivation is 108.29 U / g.
Embodiment 3
[0037] A preparation method of a beef biological tenderizer, wherein the fermentation medium formula is:
[0039] The remaining steps are the same as in Example 1, and the enzyme activity of the beef bio-tenderizer obtained after cultivation is 93.21 U / g.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Property
Measurement
Unit
concentration
aaaaa
aaaaa
Login to View More
Abstract
The invention discloses a biological type beef tendering agent as well as a preparation method and application thereof. The biological type beef tendering agent is prepared from a bacillus amyloliquefaciens strain through activation and fermentation culture, wherein the bacillus amyloliquefaciens strain is bacillus amyloliquefaciens D7 (Bacillus amyloliquefaciens), is preserved in China General Microbiological Culture Collection Center on November 10, 2016 and has the preservation number of CGMCC NO.13250. The preparation method of the biological type beef tendering agent comprises the following steps: inoculating a bouillon culture medium with the bacillus amyloliquefaciens strain to obtain activating liquid; and inoculating a fermentation culture medium with the activating liquid, and carrying out fermentation culture, so as to obtain the biological type beef tendering agent. The biological type beef tendering agent provided by the invention is used for tendering meat products, and the effect is obvious, safe and controllable.
Description
technical field [0001] The invention relates to the technical field of microorganisms, in particular to a biological tenderizer for beef and its preparation method and application. Background technique [0002] With the change of people's meat structure and the continuous improvement of living standards, beef is more and more favored by consumers, and the tenderness of beef is the most important factor affecting consumers' evaluation of beef quality, so the relevant research on meat tenderization It has gradually become one of the hot spots that people pay attention to. There are many studies on post-mortem tenderization at home and abroad, especially enzymatic tenderization. At present, papain, ficin and bromelain are the most widely used and achieved results, which can degrade myofibrils and improve the tenderness of meat. However, due to the limitation of raw materials, this kind of protease has certain difficulties in practical application due to cost problems. Therefo...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.