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Heparin sodium extracting method

An extraction method, the technology of heparin sodium, applied in the field of heparin sodium extraction, can solve the problems of insufficient improvement in the yield of heparin sodium, unreasonable control of enzymatic hydrolysis temperature and salinity, etc., to achieve increased yield and improved enzymatic hydrolysis The effect of off-recovery rate

Inactive Publication Date: 2017-06-09
HENAN ZHONGPIN FOOD IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to increase the productive rate of heparin sodium, there have been some studies and improvements aimed at the enzymatic hydrolysis process. For example, Chinese patents CN10600875A and CN104448049A both disclose the method of secondary enzymatic hydrolysis to extract heparin sodium from pig casings, but the above-mentioned secondary During the enzymatic hydrolysis process, the control of enzymatic hydrolysis temperature, salinity, etc. is not reasonable enough, so that the increase in the yield of heparin sodium is not significant enough

Method used

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  • Heparin sodium extracting method

Examples

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Effect test

Embodiment 1

[0037] A method for extracting heparin sodium, the specific operation steps are:

[0038] 1) Preparation of pig intestinal mucosa: take each healthy pig small intestine with an average weight of 1.25kg, carefully wash with clean water to remove internal and external dirt and external skin fat, and obtain clean pig intestines, mince the clean pig intestines into mince, Pig intestinal mucosa;

[0039] 2) One-time enzymatic hydrolysis: pump the prepared pig intestinal mucosa into the enzymatic hydrolysis tank, start stirring and pump concentrated brine until the salinity is 4.2 degrees, add 2.5mol / L sodium hydroxide solution until the pH is 9.0, and obtain a mixed solution , open the heating valve, and when the mixture is heated to 33°C, add 4.2g of alkaline protease according to the amount of each pig intestine, and continue to heat up to 38°C, close the heating valve, retest the pH value, and supplement Add 2.5mol / L sodium hydroxide solution until the pH is 9.0, keep stirring ...

Embodiment 2

[0050] A method for extracting heparin sodium, the specific operation steps are:

[0051] 1) Preparation of pig intestinal mucosa: take each healthy pig small intestine with an average weight of 1.25kg, carefully wash with clean water to remove internal and external dirt and external skin fat, and obtain clean pig intestines, mince the clean pig intestines into mince, Pig intestinal mucosa;

[0052] 2) One-time enzymatic hydrolysis: pump the prepared pig intestinal mucosa into the enzymatic hydrolysis tank, start stirring and pump concentrated brine until the salinity is 4.0 degrees, add 2.2mol / L sodium hydroxide solution until the pH is 8.8, and obtain a mixed solution , open the heating valve, and when the temperature of the mixed solution is raised to 31°C, add 4g of alkaline protease according to the amount of each pig intestine, and continue to heat up to 36°C, close the heating valve, retest the pH value, and add 2.2mol / L sodium hydroxide solution to pH 8.8, heat preser...

Embodiment 3

[0063] A method for extracting heparin sodium, the specific operation steps are:

[0064] 1) Preparation of pig intestinal mucosa: take each healthy pig small intestine with an average weight of 1.25kg, carefully wash with clean water to remove internal and external dirt and external skin fat, and obtain clean pig intestines, mince the clean pig intestines into mince, Pig intestinal mucosa;

[0065] 2) One-time enzymatic hydrolysis: pump the prepared pig intestinal mucosa into the enzymatic hydrolysis tank, start stirring and pump concentrated brine until the salinity is 4.5 degrees, add 2.4mol / L sodium hydroxide solution until the pH is 9.2, and obtain a mixed solution , open the heating valve, and when the mixture is heated to 35°C, add 4.5g of alkaline protease according to the amount of each pig intestine, and continue to heat up to 40°C, close the heating valve, retest the pH value, and supplement Add 2.5mol / L sodium hydroxide solution until the pH is 9.2, heat and stir ...

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Abstract

The invention relates to the technical field of deep processing of agricultural and sideline products, and specifically relates to a heparin sodium extracting method. The heparin sodium extracting method comprises the following steps: performing first enzymolysis under the salinity of 4.0-4.5 degrees and the temperature of 38+ / -2 DEG C; then performing secondary enzymolysis under the salinity of 3.0-3.5 degrees and the temperature of 55+ / -1 DEG C; and then sequentially carrying out protein denaturation, absorbing, washing, eluting, dewatering and desalting, and drying after the two enzymolysis. According to the heparin sodium extracting method, the temperature of 38+ / -2 DEG C and the salinity of 4.0-4.5 degrees are creatively selected for the first enzymolysis, and the temperature of 55+ / -1 DEG C and the salinity of 3.0-3.5 degrees are selected for the second enzymolysis, so that the heparin sodium yield reaches 1490-1510 items per hundred million units; compared with a traditional first enzymolysis method and a secondary enzymolysis method disclosed in the prior art, the heparin sodium extracting method has the advantages that the enzymolysis dissociation recovering rate is increased, so that the heparin sodium yield is increased.

Description

technical field [0001] The invention relates to the technical field of deep processing of agricultural by-products, in particular to a method for extracting heparin sodium. Background technique [0002] Heparin sodium is a strongly negatively charged mucopolysaccharide with an average molecular weight of 15,000 Daltons. It is used to prevent and treat thrombosis or embolism diseases; various disseminated intravascular coagulation; also used in hemodialysis, extracorporeal circulation, catheterization, microvascular Anticoagulant treatment of certain blood samples or instruments during operations such as surgery. Crude heparin sodium is the raw material of purified heparin sodium, which is extracted from the small intestinal mucosa of pigs and sheep or bovine lungs. [0003] At present, the crude heparin sodium extraction production process adopts enzymatic hydrolysis---resin adsorption method, and the average production rate in the industry is generally 1600-1680 pig small ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
CPCC08B37/0003C08B37/0075
Inventor 屈建兴张建林陆学君谢新华王锐
Owner HENAN ZHONGPIN FOOD IND
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