Primers and probe set of cross primer amplification-immunochromatography test paper combination method for detecting pseudorabies virus wild strain and cross primer amplification-immunochromatography test paper combination kit for detecting pseudorabies virus wild strain

Abstract

The invention discloses primers and a probe set of a cross primer amplification-immunochromatography test paper combination method for detecting a pseudorabies virus wild strain and a cross primer amplification-immunochromatography test paper combination kit for detecting the pseudorabies virus wild strain. The invention first discloses the primers and the probe set of the cross primer amplification-immunochromatography test paper combination method for detecting the pseudorabies virus wild strain, which comprise two outer side primers, a cross primer and two probes. The invention further discloses the cross primer amplification-immunochromatography test paper combination kit for detecting the pseudorabies virus wild strain, which comprises a cross primer amplification agent and an immunochromatographic test strip. Virus genomic DNA is taken as a template, a CPA reaction system is built through the cross primer amplification agent and is used for amplification, and a sample of an amplified product is loaded onto the immunochromatographic test strip, so as to realize result determination. The primers, the probe set and the kit, provided by the invention, can realize specific detection on the pseudorabies virus wild strain, the sensitivity and specificity are good, the visual degree is high, the operation is simple and convenient, and the primers, the probe set and the kit are suitable for on-site detection of pseudorabies viruses.

Description

technical field [0001] The present invention relates to the detection of pseudorabies virus wild strains, in particular to the detection of pseudorabies virus wild strains cross-primer amplification-test strip combination primers and probe sets and kits, belonging to pseudorabies virus wild strains detection field. Background technique [0002] Pseudorabies (PR) is an acute infectious disease caused by pseudorabies virus (PRV) that can infect pigs and other animals. The disease can lead to death of piglets, respiratory diseases of shelf pigs, abortion of sows, etc. PRV, also known as Ojecki's virus or porcine herpesvirus type 1, belongs to the Herpesviridae family, with a genome length of 143kb (Nauwynck, H., Glorieux, S., Favoreel. H., Pensaert, M., 2007. Cell biological and Molecular characteristics of pseudorabies virus infections in cell cultures and in pigs with emphasis on the respiratory tract. Vet. Res. 38, 229–241; Mettenleiter, T.C., 2000. Aujeszky's disease (Pse...

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Problems solved by technology

CPA amplification products can be combined with fluorescent dyes that specifically bind to DNA double strands to emit fluorescence, which can be judged according to the fluorescence intensity, but the fluorescent signal is difficult to be accurately identified by the naked eye

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