Simple and rapid thin-layer chromatography for the identification of licorice, licorice inflate and glabra
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A technology of Glycyrrhiza bulging and Glycyrrhiza glabra, applied in the field of reversed-phase thin-layer chromatography, can solve the problems that the appearance and properties of licorice are not easy to distinguish, etc.
Active Publication Date: 2018-01-30
BEIJING INST FOR DRUG CONTROL
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[0005] On the one hand, due to the different chemical components of licorice derived from Glycyrrhiza uralensis Fish., Glycyrrhiza inflate Bat. or Glycyrrhiza glabra L., on the other hand, physicians of all ages only used Glycyrrhiza uralensis Fish. One source of licorice, but the appearance of three sources of licorice is not easy to distinguish
[0006] Although technically speaking, the identification and distinction of the three sources of licorice can be achieved through sophisticated analytical instruments such as high-performance liquid chromatography, but there is no simple and easy method to simultaneously realize the identification and differentiation of the three sources of licorice
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Embodiment 1
[0047] Embodiment 1: The method for distinguishing Glycyrrhizae Glycyrrhizae, Glycyrrhiza inflata, and Glycyrrhiza Glabra by reversed-phase thin-layer chromatography.
[0048] 1) Preparation of the reference medicinal material solution:
[0049] Weigh 1g each of licorice (licorice) and licorice (glycyrrhiza inflata) reference herbs, respectively, and place them in conical flasks with stoppers, add 100ml of 70% ethanol (volume ratio of ethanol in aqueous solution), seal it tightly, and process with ultrasonic (power 500W, frequency 40kHz) for 30 minutes, let cool, filter, get the continued filtrate, as the reference medicinal material solution, for subsequent use;
[0050] 2) Preparation of the medicinal material test solution:
[0051] Use a high-speed universal pulverizer to pulverize the dried licorice, licorice bulging and licorice glabra medicinal materials respectively, and sieve through a 60-mesh sieve to obtain medicinal material powder;
[0052] Weigh 1g of licoric...
Embodiment 2
[0063] Embodiment 2: The method for distinguishing Glycyrrhizae Glycyrrhizae, Glycyrrhiza inflate, and Glycyrrhiza Glabra by reversed-phase thin-layer chromatography.
[0064] 1) Preparation of the reference medicinal material solution:
[0065] Weigh 2 g each of Glycyrrhizae (Glycyrrhiza Glycyrrhizae) and Glycyrrhiza inflata (Glycyrrhiza inflata) as the reference medicinal material, respectively, and place them in conical flasks with stoppers, add 150 ml of 80% methanol (methanol accounts for the volume ratio of the aqueous solution), close the plug, and process with ultrasound (power 500W, frequency 40kHz) for 30 minutes, let cool, filter, get the continued filtrate, as the reference medicinal material solution, for subsequent use;
[0066] 2) Preparation of the medicinal material test solution:
[0067] Use a high-speed universal pulverizer to pulverize the dried licorice, licorice bulging and licorice glabra medicinal materials respectively, and sieve through a 60-mesh ...
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Abstract
The invention relates to a thin-layer chromatography method for identifying liquorice, glycyrrhiza inflate and glycyrrhiza glabra easily and rapidly. Specifically, the method includes: 1), taking a liquorice reference crude herb and a glycyrrhiza inflate reference crude herb, and adding methyl alcohol or an ethanol solution to the reference crude herbs to prepare reference crude herb solutions; 2), taking coarse powder of the three herbs of liquorice, glycyrrhiza inflate and glycyrrhiza glabra prior to performing ultrasonic processing by adding a solvent to obtain test solutions of liquorice, glycyrrhiza inflate and glycyrrhiza glabra; 3), respectively absorbing the proper reference crude herb solutions and test solutions and applying samples on a reversed-phase thin layer plate; 4), performing development by taking 0.1% of a phosphoric solution and ethyl alcohol in proportion as a developing solvent and taking out after development prior to performing drying; 5), identifying the liquorice, glycyrrhiza inflate and glycyrrhiza glabra by comparison of spots in a chromatogram. The different spots are displayed in the three sources of the liquorice in the chromatogram, and the method is high in specificity and capable of identifying the liquorice, glycyrrhiza inflate and glycyrrhiza glabra accurately.
Description
technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, in particular to the technical field of identification of traditional Chinese medicinal materials, in particular to a reversed-phase thin-layer chromatography method for simply and quickly identifying licorice, licorice inflate and licorice glabra. Background technique [0002] Licorice is a commonly used traditional Chinese medicine, first recorded in "Shen Nong's Materia Medica", listed as top grade. It has the effects of invigorating the spleen and replenishing qi, clearing heat and detoxifying, moistening the lungs and relieving cough, relieving spasms and relieving pain, and harmonizing various medicines. Glycyrrhizae has become the largest Chinese herbal medicine used in Chinese herbal medicines. According to the licorice species listed on page 86 of the 2015 edition of "Chinese Pharmacopoeia", licorice is the dried root and rhizome of Glycyrrhiza uralensis Fish., Gly...
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