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A probe system for biosensor signal amplification and its application

A biosensor and signal amplification technology, applied in the field of electrochemical biosensors, can solve the problems of low sensitivity, expensive equipment, complicated operation, etc., and achieve the effect of high sensitivity

Active Publication Date: 2019-10-01
中山和芯生物技术有限公司
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Problems solved by technology

[0004] The purpose of the present invention is to provide a probe system for signal amplification of biosensors, aiming to solve the problems of low sensitivity, complex operation or expensive equipment of existing biosensors

Method used

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  • A probe system for biosensor signal amplification and its application
  • A probe system for biosensor signal amplification and its application
  • A probe system for biosensor signal amplification and its application

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Embodiment 1

[0047] Embodiment 1, the detection method based on the electrochemical signal amplification of DNA hybridization tandem probe detects Escherichia coli gene, comprises the following steps:

[0048] S11. Probe design: by comparing and analyzing the sequence of the Escherichia coli 16srRNA gene sequence on the bacterial genome already in the Genebank database and related nucleic acid sequences reported in domestic and foreign published literature, the conserved fragment of the Escherichia coli 16srRNA gene sequence For the target gene, select a highly conserved segment with no secondary structure, and use software to manually design capture probes, target gene sequences, signal probes, and linker probes according to the basic principle of no mismatch between primers and probes. The multiple sets of probes designed above were used to detect the above-mentioned synthetic target gene sequences, and after repeated tests, the best probe combinations with good specificity, sensitivity a...

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Abstract

The invention relates to a field of electrochemical biosensor, and provides a probe system for the signal amplification of a biosensor. The probe system comprises a joint probe and a signal amplification structure connected to the joint probe. The joint probe comprises a first region, whose 3' terminal is complementarily combined with a target gene and a second region connected to the signal amplification structure, wherein the second region comprises 2-4 identical sequence units, a base partition is arranged between neighbored sequence units, the signal amplification structure comprises 2-4 independent signal amplification units, each signal amplification unit is composed of a signal probe and a serial marker connected to the 5' terminal of the signal probe, the signal probe is complementarily connected to the sequence unit, and the serial marker is composed of 2-4 serially connected markers. The invention also provides an electrochemical signal amplification detection method based on a DNA / RNA hybridization concatenated probe.

Description

technical field [0001] The invention belongs to the field of electrochemical biosensors, in particular to a probe system for signal amplification of biosensors and its application. Background technique [0002] Ultra-sensitive nucleic acid detection technology is widely used in the detection and diagnosis of viruses, pathogenic bacteria, genetic diseases and other fields. Such as fluorescent quantitative PCR technology, rolling circle amplification (RCA) and other trace nucleic acid detection technologies are based on polymerase chain reaction and fluorescent signal detection methods to achieve the quantification or detection of trace nucleic acids, but these detection methods have complex operation steps and optical signal probes. Expensive, requiring specific testing equipment and other issues. Electrochemical sensing technology has always been the most widely used biomedical and chemical analysis detection platform, with the advantages of simplicity, portability, low cos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30
Inventor 李科铮吴宇亮
Owner 中山和芯生物技术有限公司
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