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A method for the preparation of glutathione-imprinted sensors by magnetron-induced self-assembly and rapid film formation

A glutathione and self-assembly technology, applied in the field of analysis and detection, to achieve the effects of good conductivity, improved selectivity and enhanced sensitivity

Inactive Publication Date: 2017-02-22
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the self-assembly of magnetic control-induced ternary composite nanoparticles for the preparation of glutathione molecularly imprinted electrochemical sensors has not been reported.

Method used

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  • A method for the preparation of glutathione-imprinted sensors by magnetron-induced self-assembly and rapid film formation
  • A method for the preparation of glutathione-imprinted sensors by magnetron-induced self-assembly and rapid film formation
  • A method for the preparation of glutathione-imprinted sensors by magnetron-induced self-assembly and rapid film formation

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Experimental program
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Effect test

Embodiment 1

[0050] a) Electrode pretreatment: magnetic glassy carbon electrode (Φ=5mm) through 0.05μm A1 2 o 3 After the suspension was polished, it was ultrasonically cleaned with absolute ethanol and deionized water for 2 min respectively.

[0051] b) Fe 3 o 4 Preparation of @PANI-rGO composite nanoparticles: In a 100mL three-necked bottle, take 6.5mg mL -1 Graphene oxide (GO) 3mL was dissolved in 40mL deionized water and ultrasonicated for 1h. Then add the prepared 0.5g Fe 3 o 4 @PANI composite nanoparticles, pre-assemble reaction at 80°C for 1h, add 0.3mL reducing agent hydrazine hydrate, and stir at constant temperature for 10h. Magnetic separation, washing to neutrality, and vacuum drying for 24 hours to obtain Fe 3 o 4 @PANI-rGO tri-primitive composite nanoparticles.

[0052] c) Pre-assembly of composite nanoparticles and imprinted layer hydrogen-bonded complex: preparation containing 2.5mmol L -1 GSH (glutathione), 0.3mg L -1 Fe 3 o 4 @PANI-rGO composite nanoparticles...

Embodiment 2

[0057] a) Electrode pretreatment: magnetic glassy carbon electrode (Φ=5mm) through 0.05μm A1 2 o 3 After the suspension was polished, it was ultrasonically cleaned with absolute ethanol and deionized water for 2 min respectively.

[0058] b) Fe 3 o 4 Preparation of @PANI-rGO composite nanoparticles: in a 100mL three-necked bottle, 6.5mg mL -1 Graphene oxide (GO) 6mL was dissolved in 40mL deionized water and ultrasonicated for 1h. Then add the prepared 0.5g Fe 3 o 4 @PANI composite nanoparticles, pre-assemble reaction at 80°C for 1h, add 0.2mL reducing agent hydrazine hydrate, and stir at constant temperature for 10h. Magnetic separation, washing to neutrality, and vacuum drying for 24 hours to obtain Fe 3 o 4 @PANI-rGO tri-primitive composite nanoparticles.

[0059] c) Pre-assembly of composite nanoparticles and imprinted layer hydrogen-bonded complex: preparation containing 2.5mmol L -1 GSH (glutathione), 0.3mg L -1 Fe 3 o 4 @PANI-rGO composite nanoparticles, 75m...

Embodiment 3

[0064] a) Electrode pretreatment: magnetic glassy carbon electrode (Φ=5mm) through 0.05μm A1 2 o 3 After the suspension was polished, it was ultrasonically cleaned with absolute ethanol and deionized water for 2 min respectively.

[0065] b) Fe 3 o 4 Preparation of @PANI-rGO composite nanoparticles: In a 100mL three-necked bottle, take 6.5mg mL -1 Graphene oxide (GO) 5mL was dissolved in 40mL deionized water, and sonicated for 1h. Then add the prepared 0.5g Fe 3 o 4 @PANI composite nanoparticles, pre-assemble reaction at 80°C for 1h, add 0.3mL reducing agent hydrazine hydrate, and stir at constant temperature for 10h. Magnetic separation, washing to neutrality, and vacuum drying for 24 hours to obtain Fe 3 o 4 @PANI-rGO tri-primitive composite nanoparticles.

[0066] c) Pre-assembly of composite nanoparticles and imprinted layer hydrogen-bonded complex: preparation containing 2.5mmol L -1 GSH (glutathione), 0.3mg L -1 Fe 3 o 4 @PANI-rGO composite nanoparticles, 12...

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Abstract

The invention discloses a method for preparing a glutathione imprinted sensor through magnetic-control induced self-assembly and quick film formation. The method sequentially comprises six steps as follows: electrode pretreatment, preparation of Fe3O4@PANI-rGO composite nanoparticles, joint pre-assembly of the composite nanoparticles and an imprinted layer compound, electrode modification through magnetic-control induced self-assembly, imprinted polymer formation through electric polymerization and template molecule elution. The preparation method is simple and convenient to operate, the price is low, the response is sensitive, the interference rejection is high, stability and repeatability are good, glutathione in blood plasma can be detected efficiently, flexibly and rapidly, and the method has important significance on diagnosis and treatment for clinical related diseases.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a method for rapidly forming a film by magnetic control induced self-assembly for preparing a glutathione imprinted sensor. Background technique [0002] Glutathione (GSH) is an important non-protein sulfhydryl compound existing in living organisms. As an important biological reducing agent, it plays an important role in living organisms, such as detoxification, maintaining cell stability, Anti-oxidation / nitrosation damage, etc. [1] . The elimination of GSH is closely related to many diseases in the human body, such as diabetes, AIDS and neurodegenerative diseases, etc. [2] . Therefore, the determination of the content of GSH in biological samples is of great significance and practical value for the diagnosis and treatment of related diseases. [0003] Electrochemical sensors have high sensitivity, simple design, low price, good stability and reusab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/327
Inventor 周学敏姜慧君朱婉莹江郭一李昺之蔡奇志
Owner NANJING MEDICAL UNIV
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