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Electrochemical aptamer sensor for detecting insulin as well as preparation method and use method of electrochemical aptamer sensor

An aptamer sensor, insulin technology, applied in the field of sensors, can solve the problems of large volume, lack of flexibility, etc., and achieve the effect of large specific surface area, low cost, and excellent conductivity

Pending Publication Date: 2022-02-11
SHANGQIU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

GCE has the advantages of good electrical conductivity and high chemical stability, and has been widely used in electroanalytical chemistry, but it has the problems of large volume and lack of flexibility.

Method used

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  • Electrochemical aptamer sensor for detecting insulin as well as preparation method and use method of electrochemical aptamer sensor
  • Electrochemical aptamer sensor for detecting insulin as well as preparation method and use method of electrochemical aptamer sensor
  • Electrochemical aptamer sensor for detecting insulin as well as preparation method and use method of electrochemical aptamer sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A method for preparing an electrochemical aptasensor for detecting insulin, the steps are as follows:

[0042] (1) Preparation of AuNPs-Apt probe

[0043] 50mL 0.01% (w / v) HAuCl 4 The aqueous solution was heated to boiling under stirring, and then 1 mL of 2% (w / v) sodium citrate solution was quickly added, and then the mixed solution was refluxed and boiled for 15 minutes. The color of the solution changed from light yellow at the beginning to purple, and finally to wine red. , indicating the synthesis of AuNPs. The resulting AuNPs solution was cooled to room temperature, transferred to a brown glass bottle, and stored in a refrigerator at 4 °C for future use. Add 30 μL, 100 μM Apt solution to 570 μL AuNPs solution, incubate at 4 °C for 16 h, centrifuge and wash three times, redisperse into 600 μL ultrapure water to obtain AuNPs-Apt probe solution, and store in a refrigerator at 4 °C. The sequence for recognizing the insulin aptamer Apt is: 5'-SH-GGT GGT GGG GGG GGT ...

Embodiment 2

[0052] A method for preparing an electrochemical aptasensor for detecting insulin, the steps are as follows:

[0053] (1) Preparation of AuNPs-Apt probe

[0054] 50mL 0.01% (w / v) HAuCl 4 The aqueous solution was heated to boiling under stirring, and then 1 mL of 2% (w / v) sodium citrate solution was quickly added, and then the mixed solution was refluxed and boiled for 15 minutes. The color of the solution changed from light yellow at the beginning to purple, and finally to wine red. , indicating the synthesis of AuNPs. The resulting AuNPs solution was cooled to room temperature, transferred to a brown glass bottle, and stored in a refrigerator at 4 °C for future use. Add 30 μL, 100 μM Apt solution to 570 μL AuNPs solution, incubate at 4 °C for 16 h, centrifuge and wash three times, redisperse into 600 μL ultrapure water to obtain AuNPs-Apt probe solution, and store in a refrigerator at 4 °C. The sequence recognizing the insulin nucleic acid aptamer Apt is: 5'-SH-GGT GGT GGG...

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Abstract

The invention belongs to the field of sensors, and relates to a nano material modified graphene printing electrode and an aptamer sensor, in particular to an electrochemical aptamer sensor for detecting insulin as well as a preparation method and a use method of the electrochemical aptamer sensor. A nucleic acid aptamer fragment Apt is introduced; when a target object insulin exists, Apt is combined on the surface of an electrode to form a compound, the function of a complete aptamer is shown; the distance between gold nanoparticles and the surface of the electrode is changed before and after target molecules are recognized, so that the change of an electrochemical signal is obtained. The detection limit of a laser printed graphene electrode is 22.7 fM, the detection limit of a glassy carbon electrode is 9.8 fM, the design scheme of the sensor is constructed on a disposable, portable and low-cost laser printed graphene electrode, so that commercialization of the sensor is facilitated, and the sensor has a wide prospect in the aspect of realizing portable sensing equipment for detecting insulin.

Description

technical field [0001] The invention belongs to the field of sensors, and relates to a nanomaterial-modified graphene printing electrode and an aptamer sensor, in particular to an electrochemical aptamer sensor for detecting insulin and a preparation and use method thereof. Background technique [0002] As the hormone that regulates blood sugar in the human body, insulin is involved in regulating sugar metabolism, maintaining blood sugar balance, and promoting glucose uptake. Impaired insulin secretion is an important cause of diabetes, cancer and neurodegenerative diseases. Diabetes is one of the most common chronic diseases in almost all countries, and the number of diabetic patients is increasing due to changes in lifestyle. People with diabetes often have high blood sugar, which can lead to serious complications such as kidney failure, heart attack, stroke, etc. Monitoring the concentration of insulin can be used as an indicator for early detection of diabetes and the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3277G01N27/3278G01N27/48
Inventor 常竹刘瑾瑾朱碧澄朱旭郝远强张银堂徐茂田
Owner SHANGQIU NORMAL UNIVERSITY
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