Application of lysophosphatidic acid serving as marker in detecting endometriosis

A technology of lysophosphatidic acid and endometrium, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of research reports, lack of LPA value and application prospects, etc., and achieve the effect of important medical value.

Inactive Publication Date: 2017-06-20
江西省妇幼保健院
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Problems solved by technology

In summary, although lysophosphatidic acid LPA is involved in regulating the occurrence and development of many diseases, there are no research reports on the value and application prospects of LPA in the diagnosis and treatment of endometriosis

Method used

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  • Application of lysophosphatidic acid serving as marker in detecting endometriosis
  • Application of lysophosphatidic acid serving as marker in detecting endometriosis
  • Application of lysophosphatidic acid serving as marker in detecting endometriosis

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Embodiment 1

[0025]After being approved by the Medical Ethics Committee of Jiangxi Provincial Maternal and Child Health Hospital and obtaining the informed consent of the patients, EDTA tubes were used to collect patients with confirmed endometriosis (30-42 years old) and those without endometriosis ( Control group) (30-42 years old) 30 blood samples were centrifuged at 1,750×g for 15 minutes at room temperature to obtain plasma. With reference to the literature method (JChromatogr B Analyt Technol Biomed and Life Sci.2008; 864 (1-2): 22-28.) to carry out sample processing and use liquid chromatography-tandem mass spectrometry in colleges and universities to determine six kinds of LPA (18:2LPA; 16: 0LPA; 20:4LPA; 18:1LPA; 22:6LPA).

[0026] The triple quadrupole ion hydrazine complex mass spectrometer was purchased from AB SCIEX Company in the United States, the liquid chromatography was from Shimadzu Company in Japan, and the LPA standard and isotope standard were purchased from Avanti Po...

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Abstract

The invention discloses application of lysophosphatidic acid serving as a biological marker in preparing a diagnostic reagent or a detection object for detecting endometriosis. Meanwhile, the invention further provides a biological marker for detecting endometriosis. The biological marker is prepared from the following six kinds of lysophosphatidic acid of 18:2 LPA, 16:0 LPA, 20:4 LPA, 18:1 LPA, 22:6 LPA and 18:0 LPA. According to the application of the lysophosphatidic acid disclosed by the invention, an ultra-high performance liquid chromatography-tandem mass spectrum method is utilized to measure levels of the six kinds of LPA in plasma of an endometriosis patient and a normal collator, and results show that the concentration levels of the six kinds of LPA in the plasma of the endometriosis patient are obviously higher than that of the normal collator. Therefore, the six kinds of LPA can serve as the biological marker of the endometriosis to be used, and sensitivity and specificity of the six kinds of LPA serving as a combined diagnosis marker can reach 92% and 86% and are both obviously higher than an existing hematological biological marker for endometriosis.

Description

technical field [0001] The invention relates to the application of lysophosphatidic acid as a biomarker in the preparation of a diagnostic reagent or detection substance for detecting endometriosis. Background technique [0002] Endometriosis (EMs) is a persistent lesion disease in which endometrial tissue with growth function appears outside the lining of the uterine cavity. The incidence of EMs in women of childbearing age is about 10%, and there is a clear upward trend. 80% of EMs are accompanied by pelvic pain, and 50% are combined with infertility. It is a common and frequently-occurring disease that seriously affects women's health. At present, clinical EMs treatment methods mainly include drug treatment, surgical treatment and a combination of the two treatments. Among them, laparoscopic surgery to remove lesions is the preferred treatment method. However, this method has a high recurrence rate. It has been reported that the third The annual recurrence rate is 7-30%,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 杨必成黄欧平汪利群王枫邹阳
Owner 江西省妇幼保健院
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