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Castanea henryi isolated culture plant regeneration method

A technology of in vitro culture and cone chestnut, which is applied in the field of plant propagation, can solve the problems of low bud proliferation coefficient and slow growth, and achieve the effect of simple operation process, large yield and excellent quality of subculture buds

Inactive Publication Date: 2017-06-23
唐春艳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for tissue culture and propagation of chestnut shoots with good growth effect and fast propagation for the problems of low bud proliferation coefficient and slow growth in the process of chestnut tissue culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Explant selection: collect semi-ligninized, robust growth of the year-old cassowary chestnut shoots as explants;

[0026] (2) Explant treatment: Rinse the explants under tap water for 10 minutes, then remove the leaves of the explants, and then soak the explants in a 0.1% mercury solution, and control the soaking time for 15 minutes. Finally, wash the medicine with pure water, cut the explants into 1.5-3cm long stems with at least one axillary bud, place them in containers according to the degree of lignification of the stems, and reserve them;

[0027] (3) Initial bud induction culture: inoculate the explants obtained in step (2) in the initial bud induction medium and cultivate 30-35 in an environment of light culture, 500 lx light, and culture temperature of 20±1℃ d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified MS medium+6-BA 4.0 mg / L+IBA 2.0 mg / L+KT 1.0 mg / L+ZT 1.5 mg / L+ VC 15 mg / L+ folic acid 10mg / L+VB 2 15 mg...

Embodiment 2

[0033] (1) Explant selection: collect semi-ligninized, robust growth of the year-old cassowary chestnut shoots as explants;

[0034] (2) Explant treatment: Rinse the explants under running water for 15 minutes, then remove the leaves of the explants, and then soak the explants in a mercury solution with a volume concentration of 0.1%, and control the soaking time for 20 minutes. Finally, wash the medicine with pure water, cut the explants into 1.5-3cm long stems with at least one axillary bud, and place them in containers according to the degree of lignification of the stems, and set aside;

[0035] (3) Initial bud induction culture: inoculate the explants obtained in step (2) in the initial bud induction medium and cultivate 30-35 in an environment of light culture, 1000 lx light, and culture temperature of 20±1℃ d, the initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified MS medium+6-BA 5.0 mg / L+IBA 3.0 mg / L+KT 1.5mg / L+ZT 1.5 mg / L+ ...

Embodiment 3

[0041] (1) Explant selection: collect semi-ligninized, robust growth of the year-old cassowary chestnut shoots as explants;

[0042] (2) Explant treatment: Rinse the explants under running water for 15 minutes, then remove the leaves of the explants, and then soak the explants in a mercury solution with a volume concentration of 0.1%, and control the soaking time for 20 minutes. Finally, wash the medicine with pure water, cut the explants into 1.5-3cm long stems with at least one axillary bud, and place them in containers according to the degree of lignification of the stems, and set aside;

[0043] (3) Initial bud induction culture: inoculate the explants obtained in step (2) in the initial bud induction medium and cultivate 30-35 in an environment of light culture, 1000 lx light, and culture temperature of 20±1℃ d, the initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified MS medium+6-BA 6.0 mg / L+IBA 2.5 mg / L+KT 2.0mg / L+ZT 2.0 mg / L+ ...

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Abstract

The invention discloses a castanea henryi isolated culture plant regeneration method, which comprises the culture work procedures of explant selection, explant treatment, initial bud induction culture, multiplication culture, strong bud culture and rooting culture; semi-lignified and strong growth current year castanea henryi tender branches are collected to be used as explants; the explants are subjected to trimming and sterilization disinfection treatment and are then inoculated into an initial bud induction culture medium to obtain initial buds; then, the initial buds are inoculated into a multiplication culture medium to be subjected to multiplication culture for 3 to 4 periods to form cluster buds; the cluster buds are inoculated into a strong bud culture medium to be cultured; finally, single buds with the height being greater than or equal to 2cm are inserted into the rooting culture medium for inducing the rooting. The method has the advantages that the seedling breeding period of the castanea henryi is shortened; the seedling breeding materials are saved; the defects of many materials required for seedling breeding, long period and the like in the conventional seedling breeding method are overcome; the production cost is greatly reduced; the production efficiency is improved; good economic benefits, social benefits and ecological benefits are realized.

Description

Technical field [0001] The invention belongs to the technical field of plant propagation, and relates to a method for in vitro culture plant regeneration of Castanea henryi. Background technique [0002] Castanea henryi (Skam) Rehd. et Wils., Castanea henryi (Skam) Rehd. et Wils., alias: Castanea henryi, sharp chestnut, arrow chestnut, rotundus, chestnut, etc., deciduous tree, up to 30 meters, diameter up to 1 meter . The leaves are alternate, ovate-lanceolate, 8-17 cm long and 2-5 cm wide, with a pointed tip, round base, and serrated leaf margins. Male inflorescences have lower leaf axils of branchlets, and female inflorescences have upper leaf axils of branchlets. The cupule is spherical, with a thorny diameter of 2-3.5 cm; the nut is solitary in a cupule and is ovoid. Flowering period from May to July, fruit period from September to October. It is widely distributed in the south of the southern slope of the Qinling Mountains and north of the Wuling Mountains in China. It i...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 唐春艳陈素云
Owner 唐春艳
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