Gene detection method for evaluating intake of nutrient elements

A technology for genetic testing and nutrient elements, applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as different, excessive supplementation, and harm to health.

Inactive Publication Date: 2017-06-23
北京东方亚美基因科技研究院有限公司
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  • Abstract
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Problems solved by technology

Most people’s intake of nutrient elements is only blindly ingested or consumed according to the dosage indicated in the health product instructions, but the absorption rate and metabolic rate of nutrient elements vary from individual to individual, and most of these nutrient elements are trace amounts required by the human body Elements, the individual's own requirements for them are very small, and a little carelessness can easily lead to excessive supplementation
Insufficient intake will not achieve the desired effect; excessive intake will not only be useless, but will endanger the health of the body. If the consequences are serious, it may also cause irreversible damage to internal organs

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  • Gene detection method for evaluating intake of nutrient elements
  • Gene detection method for evaluating intake of nutrient elements
  • Gene detection method for evaluating intake of nutrient elements

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Embodiment Construction

[0146] In order to further explain the technical means and effects adopted by the present invention to achieve the intended purpose of the invention, the specific implementation methods proposed according to the present invention will be described in detail below in conjunction with the preferred embodiments.

[0147] 1. Gene polymorphisms related to nutrient metabolism and their interpretation

[0148] 1. Related gene polymorphisms (see Table 1)

[0149]

[0150] (Table 1)

[0151] 2. Interpretation of related gene polymorphisms

[0152] 1) RBP4 gene

[0153] This gene is related to the metabolism of vitamin A in the body. The protein encoded by it belongs to the apolipoprotein superfamily and is the main carrier of retinol (vitamin A) in the blood. It is responsible for delivering vitamin A stored in the liver to surrounding tissues. Diseases associated with RBP4 gene mutations include: retinal trophic atrophy, iris defect, acne syndrome, microphthalmia, abdominal wall...

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Abstract

The invention provides a gene detection method for evaluating intake of nutrient elements. The method is characterized by comprising the steps of 1, designing a polymerase chain reaction (PCR) amplification primer and a single base extension reaction primer according to online primer design software; 2, after diluting a DNA sample to be detected, conducting PCR amplified reaction, shrimp alkaline phosphatase treatment reaction, single base extension reaction of genes to be detected, sample desalination and application of the sample to a chip; 3, analyzing the gene type of the DNA sample to be detected by means of the single nucleotide polymorphism typing detection technique; and 4, obtaining a genetic typing result according to obtained data.

Description

technical field [0001] The invention relates to a gene detection method, in particular to a gene detection method for evaluating the intake of nutrient elements. Background technique [0002] Vitamins are a class of organic compounds that are necessary in the process of human life activities. They have three characteristics: they mostly exist in natural foods; they cannot be synthesized by the human body and must be supplied by food; And the physiological requirement is small, but it is absolutely indispensable to the human body. [0003] The lack and excess of vitamin supplementation will have a greater impact on human health. Vitamins participate in all aspects of life activities - maintaining the normal function of the nervous system, participating in the synthesis and metabolism of protein and fat, and participating in the synthesis of various enzymes and antibodies, etc. Vitamin deficiency will seriously affect the normal metabolism of physiological functions, and eve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/106C12Q2600/156
Inventor 殷鑫曾沃坦郭金海刘进稳邹宇飞
Owner 北京东方亚美基因科技研究院有限公司
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