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Coreopsis tinctoria nutt extract extraction method

An extraction method and extract technology, which is applied in the field of snow chrysanthemum extract, can solve the problems of low purity of chemical components, little research on Kunlun snow chrysanthemum, and can not meet the needs of scientific research and clinical needs, so as to achieve high purity and wide application Prospect, effect of promoting effective separation

Inactive Publication Date: 2017-06-30
JIAMUSI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is still little research on Kunlun snow chrysanthemum at home and abroad, especially in terms of the main active ingredient evaluation indicators.
[0006] In the prior art, there are many methods for extracting components of snow chrysanthemum, mainly alcohol extraction and ultrasonic extraction. These extraction methods cannot effectively promote the effective separation of components of snow chrysanthemum, and the purity of the obtained chemical components is not high, resulting in widely varying therapeutic effects. Unable to meet scientific research and clinical needs

Method used

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  • Coreopsis tinctoria nutt extract extraction method
  • Coreopsis tinctoria nutt extract extraction method
  • Coreopsis tinctoria nutt extract extraction method

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1: the preparation of snow chrysanthemum extract

[0022] 1. Soaking: Weigh 100 grams of snow chrysanthemum powder, soak in 60% ethanol solution at a ratio of 1:15, and soak overnight.

[0023] 2. Ultrasonic and suction filtration: Ultrasonic the snow chrysanthemum ethanol solution soaked overnight, the temperature is 70°C, the time is 30min, and then the first suction filtration is performed. The snow chrysanthemum solid after the first suction filtration was soaked in 70% ethanol solution at a ratio of 1:15 and ultrasonicated again at a temperature of 70°C for 30 minutes, and then the second suction filtration was performed. The liquids after two suction filtrations were all poured into a flask for rotary evaporation to make an extract. Rotary evaporator (RE-3000 type, Zhengzhou Yarong Instrument Co., Ltd.), the temperature is 40°C and the speed is 100r / min (revolutions / minute). The final pH of the extract was 7.2-7.4.

Embodiment 2

[0024] Embodiment 2: The content of flavonoids in snow chrysanthemum is determined by high performance liquid chromatography

[0025] Precisely weigh 7 mg of the extract sample of Snow Chrysanthemum, put it in a 25 mL measuring bottle, add methanol to the mark, filter through a 0.45 μm microporous membrane, and perform high performance liquid chromatography detection. Chromatography using LC-20 AB Shimadzu high performance liquid chromatography (Shimadzu, Japan). Chromatographic column Inertsil ODS-SP column (4.6 mm × 150 mm, 5 μm); mobile phase is methanol-0.05% phosphoric acid aqueous solution; flow rate 0.6 mL min -1 , column temperature 25 ℃, detection wavelength 330nm, injection volume 10 μL, external standard method.

Embodiment 3

[0026] Embodiment 3: the content of chlorogenic acid in snow chrysanthemum is determined by chromatographic analysis

[0027] Precisely weigh 7 mg of the extract sample of Snow Chrysanthemum, put it in a 25 mL measuring bottle, add methanol to the mark, filter through a 0.45 μm microporous membrane, and perform high performance liquid chromatography detection. Chromatography using LC-20 AB Shimadzu high performance liquid chromatography (Shimadzu, Japan). Chromatographic column Inertsil ODS-SP column (4.6 mm × 150 mm, 5 μm); mobile phase is methanol-0.05% phosphoric acid aqueous solution; flow rate 0. 6 mL min -1 , column temperature 25 ℃, detection wavelength 330nm, injection volume 10 μL, external standard method.

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Abstract

The invention discloses a coreopsis tinctoria nutt extract extraction method, which comprises: a, soaking: weighing a certain amount of coreopsis tinctoria nutt powder, and soaking in an ethanol solution overnight according to a ratio of 1:8-20; and b, ultrasonic treatment and suction filtration: carrying out ultrasonic treatment on the soaked coreopsis tinctoria nutt-ethanol solution, carrying out first suction filtration, adding an ethanol solution to the coreopsis tinctoria nutt solid obtained after the first suction filtration according to a ratio of 10:1, soaking, carrying out ultrasonic treatment again, carrying out second suction filtration, mixing the liquids obtained from the two suction filtrations, and carrying out rotary evaporation to produce an extract, wherein the final pH value of the extract is 7.2-7.4. According to the present invention, with the extraction method, the effective separation of the coreopsis tinctoria nutt components is promoted, the purity of the three effective components in the obtained extract is high, the assisted chemical additive is not added during the extraction process, the method is safe and reliable, the clinical and scientific research needs are met, and the method has broad application prospects.

Description

technical field [0001] The invention relates to the field containing algae, moss, fungi or plants or derivatives thereof, specifically a method for extracting snow chrysanthemum extract. Background technique [0002] With the rapid development of modern social economy and the continuous improvement of people's living standards, the incidence of hyperlipidemia is getting higher and higher, and the patients tend to be young people. Hyperlipidemia is usually a common multiple disease caused by abnormal lipid metabolism in the human body. The main reason why this disease poses a serious threat to human health is that hyperlipidemia is usually associated with diabetes, hypertension, fatty liver, arterial The development of high-mortality diseases such as atherosclerosis is closely related and is an important factor in the development of these diseases. Therefore, the prevention and treatment of hyperlipidemia has received special attention in recent years and has become a resear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28
CPCA61K36/28A61K2236/333A61K2236/53
Inventor 李锦莲武冬梅王寒冰郭英雪
Owner JIAMUSI UNIVERSITY
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