Chloroalkene determinating device and detection method thereof

Abstract

The invention relates to a chloroalkene determinating device and a detection method thereof, and belongs to the technical field of environment monitoring. The chloroalkene determinating device comprises a water bath device, a biosensor device, a biomembrane device, an electromagnetic stirring device and a detection device, wherein the water bath device provides a constant-temperature environment; the biosensor device comprises an AgCl / Ag2S membrane working electrode and an Ag / AgCl reference electrode; the biomembrane device is fixed below the working electrode, and comprises two porous nitrocellulose membranes and degrading bacteria fixed between the two membranes; the electromagnetic stirring device ensures uniform mixing of a buffer solution; the detection device is used for determinating chloride ion concentration after the bacteria degrade chloroalkene, and finally converting the chloride ion concentration into the concentration of the determinated chloroalkene. The content of the chloroalkene is determinated through microbial degradation, so that secondary pollution is avoided; the chloroalkene determinating device is simple and reusable; the reaction time is very short; the detection limit is low; the reproducibility is high; the chloroalkene determinating device can resist interference caused by temperature and pH fluctuations within a certain range; efficient and accurate determination of the chloroalkene in groundwater is ensured.

Description

technical field [0001] The invention belongs to the technical field of environmental monitoring, and relates to a chlorinated olefin measuring device and a detecting method thereof. Background technique [0002] Insufficient water sources and water source pollution are the most immediate problems facing China's drinking water sources, and the problem of drinking water source pollution is particularly prominent. At present, about 97% of China's cities have groundwater pollution, and the degree is divided into serious pollution, moderate pollution and light pollution, and the basic clean groundwater is only 3%. Groundwater pollution is a phenomenon in which the quality of groundwater decreases due to changes in the chemical composition, physical properties, and biological characteristics of groundwater caused by human activities. The main causes of groundwater pollution are: direct discharge of industrial wastewater to the ground, intrusion of polluted surface water into the ...

AI Technical Summary

Problems solved by technology

Since chlorinated olefins are volatile chlorine-containing aliphatic compounds and are difficult to be directly degraded by microorganisms, there are very few studies on the determination of chlorinated olefins using biosensors

The traditional determination method is gas chromatography, which takes a long time to analyze, requires pretreatment, and the steps of solvent extraction and purge and trap are relatively complicated, and the experimental equipment is relatively expensive

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