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High-quality high-resistant starch japonica rice molecular breeding method

A technology of high resistant starch and molecular breeding, applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of high digestible starch content, low resistant starch content, high blood sugar, etc., and achieve improved Effects of Accuracy and Goal Expectation, Strong Practicality, Rapid Stability and Early Judgment

Inactive Publication Date: 2017-07-04
浙江绿巨人生物技术有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The resistant starch content of ordinary rice is too low and the digestible starch content is too high, which can easily lead to high blood sugar

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The process of the molecular breeding method for high-quality and high-resistant starchy japonica rice is as follows:

[0027] (1) In the winter and spring of 2013, in Lingshui, Hainan, F 1 15 substitute seeds;

[0028] (2) Spring and summer of 2013, Hangzhou, Zhejiang, planting F 1 15 plants, backcross with Nakazawa Xuenuo once as much as possible to obtain BC 1 f 1 93 seeds;

[0029] (3) In the winter and spring of 2014, Lingshui, Hainan, planted BC 1 f 1 Generation of 93 strains, using anther culture technology to construct a doubled haploid (DH) population;

[0030] (4) In the summer of 2014, Jinhua, Zhejiang, harvesting DH per plant 0 Substitute seeds, construct 326 DH lines;

[0031] (5) In the winter and spring of 2015, Lingshui, Hainan, planting DH per plant 1 Generating plants, extracting DNA, first using (CT)n microsatellite marker molecule-assisted selection, 95 lines were selected, and using SSIIIa CAPS markers, 16 lines were selected, the seeds of ...

Embodiment 2

[0036] The process of the molecular breeding method for high-quality and high-resistant starchy japonica rice is as follows:

[0037] (1) In the winter and spring of 2013, in Lingshui, Hainan, F 1 15 substitute seeds;

[0038] (2) Spring and summer of 2013, Hangzhou, Zhejiang, planting F 1 15 plants, backcross with Nakazawa Xuenuo once as much as possible to obtain BC 1 f 1 93 seeds;

[0039] (3) In the winter and spring of 2014, Lingshui, Hainan, planted BC 1 f 1 Generation of 93 strains, using anther culture technology to construct a doubled haploid (DH) population; the operation steps of the anther culture technology: before ear picking, the flag leaf sheath is scrubbed with 70% alcohol. Peel off the inner sheath of the flag in the ultra-clean workbench, take out the rice ears, sterilize in 10% bleach solution for 10 minutes, wash once with sterile water, soak in 0.01% citric acid solution for 10 minutes, and wash once with sterile water. Scrub hands twice with 70% a...

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Abstract

The invention discloses a high-quality high-resistant starch japonica rice molecular breeding method including the steps: with high-resistant starch indica rice as a parent, hybridizing with a high-quality japonica rice parent, and hybridizing to obtain F1 generation seeds; planting the F1 generation, back-crossing with the high-quality japonica rice parent for one time, to obtain BC1F1 generation seeds; planting BC1F1 plants, constructing a double haploid population by an anther culture technology, and harvesting DH0 generation seeds according to a single plant; planting DH1 generation plants, and selecting an excellent plant system with the amylose content ranged from 15 to 21% and the resistant starch content exceeding 10%; planting DH2 generation, and selecting a japonica-cline type excellent line; and planting a DH3 generation, and obtaining a shape-finalizing line of high-quality high-resistant starch japonica rice, namely high-quality high-resistant starch japonica rice. The bred high-quality and high-resistant starch japonica rice provides higher practicability for edible optimization of diabetes special rice and prevention of occurrence of diabetes from the source through diet.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a molecular breeding method for high-quality and high-resistance starchy japonica rice. Background technique [0002] According to statistics from the Ministry of Health, in 2010, there were about 92 million people with diabetes in my country, and 142 million people with low glucose tolerance. Rice is the traditional staple food of our country. Ordinary rice is too low in resistant starch and too high in digestible starch, which can easily lead to high blood sugar. The breeding of rice varieties with high resistant starch content is the key to eating healthy. [0003] Medical research shows that: In addition to genetic factors, chronic diseases of the blood system such as diabetes are mainly caused by daily living habits such as daily diet, and a high-sugar, high-fat, and high-protein diet is the culprit, especially the "culprit" of high sugar. ". The core of the rice s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01H4/00
CPCA01H1/02A01H1/04A01H4/008
Inventor 徐开盛舒小丽徐望之
Owner 浙江绿巨人生物技术有限公司
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