Method for pre-diluting vacuum blood collection tube and for measuring platelet number

Abstract

The invention discloses a pre-diluted vacuum blood collection tube, which comprises a tube body and a tube plug, wherein a tube cap is arranged at the outer side of the tube plug; the tube body, which is sealed, is under a vacuum state; scale marks, which are used for marking capacity of the tube body, are arranged on the outer wall of the tube body; scale values, indicated by the scale marks, range from 9mL to 10.5mL, and a minimum division value is 0.1mL; and 9mL of sodium chloride injection or compound electrolyte injection is contained in the tube body. The invention also discloses a method for measuring platelet number in a patient with EDTA-dependent pseudo-thrombocytopenia with the application of the pre-diluted vacuum blood collection tube. The pre-diluted vacuum blood collection tube provided by the invention is simple in structure; the pre-diluted vacuum blood collection tube is designed with the precise scale marks; venous blood is diluted by liquid which is free from an anticoagulation, so that autologous antigen in an anticoagulation-induced and activated platelet membrane glycoprotein GPIIb structure, and a purpose of inhibiting platelet aggregation is achieved; and the pre-diluted vacuum blood collection tube, through a mode of vacuum blood collection, simplifies operations and avoids pollution, caused by open blood collection, to operators and environment.

Description

technical field [0001] The invention belongs to the technical field of medical devices, and in particular relates to a pre-diluted vacuum blood collection tube and a method for measuring the number of platelets in patients with EDTA-dependent pseudothrombocytopenia. Background technique [0002] The incidence of EDTA-dependent pseudo-thrombocytopenia (EDTA-PTCP) is between 0.09% and 0.21%. EDTA-dependent pseudo-thrombocytopenia is due to the use of EDTA to anticoagulate the whole vein. After the blood test, the number of platelets in the patient was detected with a hematology analyzer, and the number of platelets decreased significantly over time, but the patient himself did not have a significant decrease in the number of platelets, and the accompanying clinical symptoms and signs were reexamined by other methods The number of platelets, the phenomenon of normal platelet count, is called pseudothrombocytopenia. EDTA-dependent pseudothrombocytopenia cases account for more t...

AI Technical Summary

Problems solved by technology

The method of visually counting the number of platelets using a phase microscope is the traditional reference method for platelet counting, but this method has high requirements for operators and equipment. Generally, there is no phase microscope in the laboratory, and the number of platelets is counted visually using an ordinary optical microscope. , there are easily interfered by impurities and dust in the counting tank of the blood cell counting plate, and the platelets stored in the ammonium oxalate diluent will spontaneously dissolve as the storage time prolongs, resulting in a decline in the counting result, and the counting must be completed within 1 hour , the shorter the time, the better. Microscopic visual counting method for platelet counting is affected by technical errors and inherent errors, resulting in poor reproducibility of the microscopic visual counting method for platelet counting. The same staff, the same microscope, and the same sample , the coefficient of variation of multiple counts before and after is as high as 10% to 25%, and it is no longer a reference method for platelet counting

At the same time, due to the same manufacturer, the same model, the same batch of products, and the same barrel of micro blood collection straws, there are certain differences between them, resulting in low accuracy of using micro blood collection straws to collect peripheral whole blood. There are various defects in the method of counting the number of platelets, which cannot meet the accurate and timely requirements of clinicians and patients for the test report. At the same time, the staff of the laboratory are accustomed to using automated equipment, and they are against the purely manual method of counting platelets by visual counting. At the same time, the microscope visual counting method can only count the number of platelets, other blood cells cannot be counted, other parameters of platelets cannot be measured, and a complete blood routine report cannot be issued. The patient may have blood sampling again, which leads to the use of this method in daily work. very few

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