Insect pathogenic nematode symbiotic bacterium capable of inhibiting potato black scurf and applications of insect pathogenic nematode symbiotic bacterium

A technology for potato black nevus and entomopathogenic nematodes, applied in the directions of application, chemicals for biological control, bacteria, etc., can solve problems such as infection, yield and quality hazards

Active Publication Date: 2017-07-04
黑龙江省农业科学院植物脱毒苗木研究所
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Problems solved by technology

Studies have shown that the pathogen has strong environmental adaptability and can survive the winter on tubers, soil or myce

Method used

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  • Insect pathogenic nematode symbiotic bacterium capable of inhibiting potato black scurf and applications of insect pathogenic nematode symbiotic bacterium
  • Insect pathogenic nematode symbiotic bacterium capable of inhibiting potato black scurf and applications of insect pathogenic nematode symbiotic bacterium
  • Insect pathogenic nematode symbiotic bacterium capable of inhibiting potato black scurf and applications of insect pathogenic nematode symbiotic bacterium

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specific Embodiment approach 1

[0014] Specific embodiment one: a kind of entomopathogenic nematode symbiont bacterium that suppresses potato black mole of the present embodiment, it is Xenorhabdus bovineii (Xenorhabdus bovineii) HEB01, is preserved in the common microorganism center of China Committee for Microorganism Culture Collection, The deposit address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing. The deposit date is March 9, 2016, and the deposit number is CGMCC NO.12193.

[0015] The Xenorhabdus bovineii HEB01 of the present embodiment is obtained as follows:

[0016] Entomopathogenic nematodes were isolated from soil samples collected from Harbin City by trapping the wax moth; the primary symbiotic bacteria were isolated according to the following methods:

[0017] Infect the 5th instar larvae of the wax borer with the isolated entomopathogenic nematodes, after the death of the larvae, immerse them in 75% alcohol by volume for 3 seconds, then wipe the surface of the larv...

specific Embodiment approach 2

[0020] Embodiment 2: An entomopathogenic nematode symbiotic bacterium capable of inhibiting potato black mole in this embodiment is used for preventing and treating potato black mole.

specific Embodiment approach 3

[0021] Embodiment 3: This embodiment is different from Embodiment 2 in that it uses the bacterial liquid of entomopathogenic nematode symbiotic bacteria to prevent and treat potato black mole. Others are the same as in the second embodiment.

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Abstract

The invention provides an insect pathogenic nematode symbiotic bacterium capable of inhibiting potato black scurf and applications of the insect pathogenic nematode symbiotic bacterium, relating to the insect pathogenic nematode symbiotic bacterium and the applications of the insect pathogenic nematode symbiotic bacterium. The bacterium is Xenorhabdus bovineii HEB01, is preserved in the China General Microbiological Culture Collection Center (CGMCC, located in 3, Courtyard 1, West Beichen Road, Chaoyang District, Beijing,) on Mar 9, 2016, is assigned with the accession number of CGMCC NO.12193, and is used for preventing and treating potato black scurf. The xenorhabdus bovineii HEB01 has the very strong capacity of inhibiting the mycelial growth of the potato black scurf bacterium, the capacity is enhanced along with the increase of the volume concentration, and the antibacterial effect is obvious. Meanwhile, the bacterium has the features that the requirements on the culture conditions are low, the lasting period is long, the source is environmentally friendly and non-toxic, and thus the insect pathogenic nematode symbiotic bacterium has very good development and application prospects.

Description

technical field [0001] The invention relates to an entomopathogenic nematode symbiotic bacterium and its application. Background technique [0002] China is a big potato planting country. Under the impetus of strategies such as potato staple food, my country's potato industry has developed rapidly. However, with the continuous expansion of the planting area, soil-borne diseases caused by many reasons, such as the imperfect crop rotation system and the imperfect supervision of virus-free seed potatoes, are common and aggravated year by year, seriously affecting the healthy development of the potato industry. [0003] Potato black mole is one of the most threatening soil-borne diseases in potato production. Symptoms such as mole and cracks can be formed on the surface of tubers, seriously affecting the quality and commerciality of potatoes (especially seed potatoes), causing huge economic losses. The pathogen Rhizoctonia solani can also cause canker in the seedling stage and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/01
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/01
Inventor 杨帅闵凡祥高云飞王文重魏琪董学志吕典秋王晓丹王玲
Owner 黑龙江省农业科学院植物脱毒苗木研究所
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