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Preparation method and application of functional photoacoustic probe based on assembly of gold nanoparticles

A gold nanoparticle and functional technology, applied in the field of nanomaterials, can solve the problems of large size, inability to clearly image and judge, normal tissue damage, etc., and achieve a wide range of applications

Inactive Publication Date: 2017-07-07
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Technical problem to be solved: In view of the problems that the existing imaging equipment is bulky, expensive, easy to cause damage to normal tissues, and can not be clearly imaged and correctly judged for the detection of caspase-3 in tumor cells, the present invention provides a gold nanometer-based The preparation method and application of particle-assembled functional photoacoustic probes can monitor the production of caspase-3 in tumor cells through photoacoustic imaging, and then judge the apoptosis process of tumor cells after treatment, and evaluate the effect of tumor treatment , which solves the defects of other methods that damage normal tissues and cannot be clearly imaged and judged

Method used

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  • Preparation method and application of functional photoacoustic probe based on assembly of gold nanoparticles

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Embodiment 1

[0032] A method for preparing a functional photoacoustic probe based on the assembly of gold nanoparticles, the preparation method is as follows:

[0033] Step 1. Preparation of spherical gold nanoparticles: mix 0.1wt.% chloroauric acid and ultrapure water, add 10mmol / L sodium citrate solution after heating and boiling in an oil bath at 130°C and heat to boiling, wherein chloroauric acid , the volume ratio of sodium citrate and ultrapure aqueous solution is 0.05:0.1:10, stir for 5 minutes, turn off the heat source and continue stirring for 10 minutes, and let it reach room temperature for later use.

[0034] Step 2. Preparation and purification of gold nanorod solution: a. the CTAB (cetyltrimethylammonium bromide) solution of 0.1mol / L and the chloroauric acid solution of 0.001mol / L are mixed in reaction vessel, add 0.001mol / L NaBH 4 solutions, among which CTAB solution, chloroauric acid solution and NaBH 4 The volume ratio of the solution is 1:0.1:0.1. After stirring, put it...

Embodiment 2

[0043] A method for preparing a functional photoacoustic probe based on the assembly of gold nanoparticles, the preparation method is as follows:

[0044] Step 1. Preparation of spherical gold nanoparticles: Mix 10wt.% chloroauric acid and ultrapure water, add 100mmol / L sodium citrate solution after heating and boiling in an oil bath at 150°C and heat to boiling, wherein chloroauric acid, The volume ratio of sodium citrate and ultrapure aqueous solution is 1:10:100, stir for 20 minutes, turn off the heat source and continue stirring for 20 minutes, and let it cool to room temperature for later use.

[0045] Step 2. Preparation and purification of gold nanorod solution: a. the CTAB (cetyltrimethylammonium bromide) solution of 1mol / L and the chloroauric acid solution of 0.1mol / L are mixed in the reaction vessel, add 0.1 mol / L NaBH 4 solutions, among which CTAB solution, chloroauric acid solution and NaBH 4 The volume ratio of the solution is 50:1:10. After stirring, put it in ...

Embodiment 3

[0054] A method for preparing a functional photoacoustic probe based on the assembly of gold nanoparticles, the preparation method is as follows:

[0055] Step 1. Preparation of spherical gold nanoparticles: Mix 1wt.% chloroauric acid and ultrapure water, add 40mmol / L sodium citrate solution after heating and boiling in an oil bath at 150°C and heat to boiling, wherein chloroauric acid, The volume ratio of sodium citrate and ultrapure aqueous solution is 0.1:1:10, stir for 10 minutes, turn off the heat source and continue stirring for 15 minutes, and let it cool to room temperature for later use.

[0056] Step 2. Preparation and purification of the gold nanorod solution: a. the CTAB (cetyltrimethylammonium bromide) solution of 0.1mol / L is mixed with the chloroauric acid solution of 0.01mol / L in the reaction vessel, and added 0.01mol / L NaBH 4 solutions, among which CTAB solution, chloroauric acid solution and NaBH 4 The volume ratio of the solution is 10:0.25:0.6. After stirr...

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Abstract

The invention relates to the field of nano materials, in particular to a preparation method and application of a functional photoacoustic probe based on assembly of gold nanoparticles. The preparation method of the functional photoacoustic probe includes the steps that peptide chains with one ends of -SH, the other ends of biotin and the middle parts of specific sequences DEVD are used for modification of the surfaces of the gold nanoparticles, meanwhile, functional micromolecules with one ends of -SH and the other ends of streptavidin are used for modification of the surfaces of gold nanorods, and the self-assembly process of the gold nanorods and the gold nanoparticles is completed by the specific biological coupling reaction of the streptavidin and the biotin. As an apoptosis enzyme caspase-3 can recognize and lyse the specific sequences DEVD of the peptide chains, the gold nanorods are separated from the gold nanoparticles, so that ultraviolet-visible absorption spectra and photoacoustic imaging signal strength begin to change; thus production of caspase-3 in tumor cells can be monitored with the method of photoacoustic imaging so as to judge the apoptosis process of the tumor cells after treatment, and the functional photoacoustic probe is also used for evaluating the effects of radiotherapy and chemotherapy, which has great significance in clinical medicine and disease diagnosis.

Description

technical field [0001] The invention relates to the field of nanomaterials, in particular to a preparation method and application of a functional photoacoustic probe based on assembly of gold nanoparticles. Background technique [0002] In recent years, with the continuous progress and development of nanotechnology, the research and application of noble metal nanoparticles have received more and more attention. Gold nanoparticles are a research hotspot in the field of nanomaterials, but currently only a single The properties and applications of gold nanoparticles, and the self-assembly technology to assemble nanoparticles into one-dimensional, two-dimensional, three-dimensional structures, and construct functional nano-assemblies through assembly, will be the direction of the development of nanomaterials in the future. [0003] Caspases (caspases) are an important family of signaling molecules with various tasks, and the activation of caspases is also a marker of cell damage...

Claims

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Application Information

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IPC IPC(8): A61K49/22
CPCA61K49/225A61K49/221
Inventor 张磊汪联辉王静慧沈晶晶范曲立黄维
Owner NANJING UNIV OF POSTS & TELECOMM
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