Portable microfluidic PCR instrument and gene sample fluorescence quantitative detection method

Abstract

The invention provides a portable microfluidic PCR (Polymerase Chain Reaction) instrument and a gene sample fluorogenic quantitative detection method. The portable microfluidic PCR instrument comprises a control unit, a PCR chip, a temperature control unit and a fluorescence signal acquisition unit, the temperature control unit and the fluorescence signal acquisition unit are connected with the control unit; the PCR chip comprises a substrate, a reaction chamber is formed on the end face of one side of the matrix, a sealing cover plate is covered on the reaction chamber, the reaction chamber is communicated with the outside through a capillary shaped sample inlet and a capillary shaped sample outlet on the substrate; the temperature control unit comprises a temperature detector, a heating part and a refrigerating part, the heating part and the refrigerating part heats or refrigerates the substrate in the PCR chip; an acquisition end of the fluorescence signal acquisition unit is distributed correspondingly to one side of the sealing cover plate of the PCR chip, so that a fluorescence image in the reaction chamber is acquired. The fluorescence signal acquisition unit instrument can conduct the polymerase chain reaction, meanwhile, the fluorescence signal acquisition unit acquires the reaction circulation fluorescence image, so that the fluorogenic quantitative detection of a gene sample is realized.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a portable microfluidic PCR instrument, and also relates to a method for quantitatively detecting fluorescence of gene samples based on the microfluidic PCR instrument. Background technique [0002] The basic principle of PCR (polymerase chain reaction) technology is similar to the natural replication process of DNA, and its specificity depends on oligonucleotide primers complementary to both ends of the target sequence. PCR basically consists of three basic reactions: denaturation-annealing-extension Step composition. PCR is to use DNA denaturation in vitro at a high temperature of 95°C to become a single strand. At a low temperature (often around 60°C), primers and single strands are combined according to the principle of complementary base pairing, and then the temperature is adjusted to the optimum reaction of DNA polymerase. Temperature (around 72°C), DNA polymerase synthesizes...

AI Technical Summary

Problems solved by technology

In such a heating method, the amount of sample used is at least hundreds of microliters, which has a certain thermal inertia, and the equipment itself also has thermal inertia, so the frequency of temperature switching should not be too fast

Images