Nitrariatangutoruntonoplast H<+>PPase protein gene NtVP1, encoding protein thereof, and cloning method

A technology of tonoplast membrane and protein, applied in the field of molecular biology, can solve the problem of no tongut white thorn membrane and other problems
CN106929492AInactive Publication Date: 2017-07-07CHINESE ACAD OF FORESTRY +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINESE ACAD OF FORESTRY
Publication Date
2017-07-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the field of molecular biology, provides a nitrariatangutoruntonoplast H<+>PPase protein gene NtVP1 and encoding protein thereof, and provides a method for cloning the gene and constructing a vector of the gene. The gene and the encoded protein thereof have important effect in salt stress resistance of thenitrariatangutoruntonoplast, thereby providing a new choice for cultivating plant new varieties.
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Description

technical field

[0001] The invention belongs to the field of molecular biology and relates to gene cloning and carrier construction. Background technique

[0002] tonoplast H + Transport inorganic pyrophosphatase (V-H + -PPase) is a unique acidifying vacuolar proton pump that exists in terrestrial plants and a few algae, protozoa and bacteria. The main functions of the enzyme are as follows: (1) Proton pump function: it can hydrolyze the free energy and H produced by inorganic pyrophosphate (PPi) + The proton transmembrane transporter is coupled to hydrolyze PPi into 2 Pi, and H in the cytoplasm + Pumped into the vacuole through the tonoplast membrane, it acts as a proton pump, the same as the tonoplast H + -ATPase together to form H + The electrochemical gradient across the tonoplast membrane provides the driving force for the active transport of molecules of various solutes (amino acids, sugars, cations and anions, etc.) across the tonoplast membrane. (2) Ion compart...

Claims

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