Food additive capable of inhibiting aspergillus flavus and preparation method thereof
A food additive, Aspergillus flavus technology, applied in the field of food processing, can solve the problems of reducing feed utilization, digestive system dysfunction, reducing fertility, etc., to achieve the effect of improving immunity, reducing impact, and increasing breeding income
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Embodiment 1
[0029] A preparation method of a food additive capable of inhibiting Aspergillus flavus, comprising the following steps:
[0030] (1) Centrifuge the chlorella culture solution cultivated to the stationary phase at a speed of 8000r / min for 5min, discard the supernatant to collect the precipitated chlorella cells, and add chlorella with a weight of 100% to the collected chlorella 6% distilled water by mass, oscillate and mix well, store in an ultra-low temperature refrigerator for 4-6 hours, take it out, put it in a water bath oscillator at 30-40°C for 20-30 minutes, and then ultrasonically treat it for 30-40 minutes to make the cell wall of chlorella broken;
[0031] (2) Soak the green tea with boiling water for 4-6 hours, the weight of the added boiling water is 30 times of the weight of the green tea, filter and get the green tea soaking liquid;
[0032] (3) the Enteromorpha salvaged in seawater is cleaned, dried or dried, pulverized, and passed through an 80-mesh sieve to o...
Embodiment 2
[0042] A preparation method of a food additive capable of inhibiting Aspergillus flavus, comprising the following steps:
[0043] (1) Put the chlorella culture solution that has been cultivated to the stable phase into a centrifuge and centrifuge at a speed of 8000r / min for 5min, discard the supernatant to collect the precipitated chlorella cells, and add a weight of chlorella to the collected chlorella Collect distilled water with 10% of the quality of chlorella, oscillate and mix well, store in an ultra-low temperature refrigerator for 4-6 hours, take it out, put it in a water bath oscillator at 30-40°C to dissolve for 20-30 minutes, and then ultrasonically treat it for 30-40 minutes Break down the cell wall of Chlorella;
[0044] (2) Soak the green tea with boiling water for 4-6 hours, the weight of the added boiling water is 30 times of the weight of the green tea, filter and get the green tea soaking liquid;
[0045] (3) the Enteromorpha salvaged in seawater is cleaned, ...
Embodiment 3
[0055] A preparation method of a food additive capable of inhibiting Aspergillus flavus, comprising the following steps:
[0056] (1) Put the chlorella culture solution that has been cultivated to the stable phase into a centrifuge and centrifuge at a speed of 8000r / min for 5min, discard the supernatant to collect the precipitated chlorella cells, and add a weight of chlorella to the collected chlorella Collect distilled water with 8% of the quality of chlorella, shake and mix, put it in an ultra-low temperature refrigerator for 4-6 hours, take it out, put it in a water bath oscillator at 30-40°C to dissolve for 20-30 minutes, and then ultrasonically treat it for 30-40 minutes Break down the cell wall of Chlorella;
[0057] (2) Soak the green tea with boiling water for 4-6 hours, the weight of the added boiling water is 30 times of the weight of the green tea, filter and get the green tea soaking liquid;
[0058] (3) the Enteromorpha salvaged in seawater is cleaned, dried or ...
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