A method for analyzing the enrichment content of phthalates carried by microplastics in organisms
A technology for phthalates and esters, which is applied in the field of analyzing the enrichment content of phthalates carried by microplastics in organisms, which can solve the uncertainty of toxicity assessment and the inability to know the enrichment and distribution regularity, lack of quantitative analysis, etc.
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Embodiment 1
[0047] Based on chromatography and mass spectrometry analysis technology and organic matter extraction technology to quantitatively analyze the content of microplastics carrying phthalates into the experimental mouse (CD-1), quantitative analysis of polyethylene material with a particle size of 40-50 μm ( PE) microplastics carry dioctyl phthalate (diethylhexyl phthalate, DEHP) into the mouse body content, the analysis process is as follows figure 1 As shown, the specific steps are as follows:
[0048] (1) Preparation of PE microplastics with pre-adsorbed DEHP: first prepare 50 mL of DEHP solution with a concentration of 50 μg / L, then weigh 10 mg of polyethylene microplastics (PE-MPs) with a particle size of 40-50 μm and add them to the above pollutant solution, And shake in the shaker for 48 hours, the shaker is at a temperature of 25° C. and 200 rpm. After the above reaction, use a 0.22 μm pore size hydrophilic glass fiber membrane (PTEF) to separate the microplastics in the...
Embodiment 2
[0078] Based on chromatography and mass spectrometry analysis technology and organic matter extraction technology to quantitatively analyze the content of microplastics carrying phthalates into the body of experimental mice (CD-1), quantitative analysis of polyethylene materials with a particle size of 1-5 μm ( PE) microplastics carry di-n-butyl phthalate (di-n-butyl Phthalate, DBP) into the mouse body content, the analysis process is as follows figure 1 As shown, the specific steps are as follows:
[0079] (1) Preparation of PE microplastics pre-adsorbed with DBP: first prepare 50 mL of DBP solution with a concentration of 5 μg / L, then weigh 10 mg of polyethylene microplastics (PE-MPs) with a particle size of 1-5 μm and add them to the above pollutant solution, And shake in the shaker for 96 hours, the shaker is at a temperature of 25° C. and 200 rpm. After the above reaction, use a 0.22 μm pore size hydrophilic glass fiber membrane (PTEF) to separate the microplastics in th...
Embodiment 3
[0109] Based on chromatography and mass spectrometry analysis technology and organic matter extraction technology to quantitatively analyze the content of microplastics carrying phthalates into the body of experimental rats (SD), quantitative analysis of polyethylene material (PE) with a particle size of 10-30 μm The content of microplastics carrying diethyl phthalate (DEP) into the rat body, the analysis process is as follows figure 1 As shown, the specific steps are as follows:
[0110] (1) Preparation of PE microplastics pre-adsorbed with DEP: first prepare 50 mL of DEP solution with a concentration of 25 μg / L, then weigh 10 mg of polyethylene microplastics (PE-MPs) with a particle size of 10-30 μm and add them to the above pollutant solution, And shake in the shaker for 72 hours, the shaker is at a temperature of 25° C. and 200 rpm. After the above reaction, the microplastics in the solution were separated using a hydrophilic glass fiber membrane (PTEF) with a pore size o...
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