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Insect stratiomyiid intestinal tract derived serratia marcescens BSFL06 and application thereof

A technology of Serratia marcescens and BSFL-6, which is applied in the direction of bacterial medical raw materials, bacteria, antibacterial drugs, etc., to achieve the effect of promoting the growth of soldier flies

Inactive Publication Date: 2017-07-28
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention isolates and obtains a strain of Serratia marcescens BSFL-6, a symbiotic bacterium in the intestinal tract of the soldier fly, which has the ability to enhance the ability of the soldier fly to resist and degrade tetracycline in the process of transforming materials, and is effective against harmful bacteria. Staphylococcus (S.Aureus) and Escherichia coli (E.coli) have antibacterial activity and are a soldier fly intestinal probiotic. At present, there is no degradable antibiotic isolated from the insect gut in the world that can interact with insect soldier flies. Insect gut bacteria report symbiotically degrading antibiotics

Method used

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  • Insect stratiomyiid intestinal tract derived serratia marcescens BSFL06 and application thereof
  • Insect stratiomyiid intestinal tract derived serratia marcescens BSFL06 and application thereof
  • Insect stratiomyiid intestinal tract derived serratia marcescens BSFL06 and application thereof

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Experimental program
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Effect test

Embodiment 1

[0021] Isolation and Identification of Serratia marcescens BSFL-6 from Soldier Fly Gut

[0022] The applicant used LB medium containing 40 mg / L tetracycline to isolate a strain of Serratia marcescens BSFL-6 from the intestinal grinding liquid of soldier flies transformed with tetracycline-contaminated materials, which produces red pigment. This strain was released in 2017 On February 28, 2017, it was sent to the China Center for Type Culture Collection for preservation. The classification name: Serratia marcescens (Serratia marcescens) BSFL-6, the preservation number: CCTCC NO: M 2017073, and the address: Wuhan University, Wuhan, China. The physiological and biochemical characteristics and culture characteristics of Serratia marcescens BSFL-6 are shown in Table 1.

[0023] Table 1 Physiological and biochemical characteristics and culture characteristics of Serratia marcescens BSFL-6

[0024]

[0025] "+" is positive and "-" is negative.

Embodiment 2

[0027] In vitro culture of Serratia marcescens BSFL-6:

[0028] Serratia marcescens BSFL-6 was activated by streaking on the plate, and a single colony on the plate was inoculated into a Erlenmeyer flask containing 100 mL of LB medium. The culture temperature was 28°C, the shaker speed was 180 rpm, and the culture time was 12 hours. Obtain Serratia marcescens BSFL-6 fermentation bacterium suspension (the present invention or be called for short BSFL-6 bacterium suspension), the effective bacterial concentration of this bacterium suspension is 10 8 cfu / ml (OD600 value 1.5).

Embodiment 3

[0030] In vitro degradation of tetracycline by Serratia marcescens BSFL-6:

[0031] 500 μL BSFL-6 bacterial suspension (effective bacterial concentration is 10 8 cfu / ml) were inoculated into Erlenmeyer flasks containing 200 mL of LB medium containing 20, 40, 80 and 160 mg / L tetracycline respectively. The culture temperature was 28°C, the shaker speed was 180 rpm, and the culture time was 96 hours. The test contents and results were as follows :

[0032] 1) Detect its growth curve, the result is as follows figure 1 As shown, with the increase of tetracycline concentration, the adaptation period of Serratia marcescens BSFL-6 gradually prolongs, but enters the logarithmic growth phase after the adaptation period, and reaches 10 8 cfu / ml (OD600 value 1.5); Serratia marcescens BSFL-6 can tolerate growth even at the concentration of 160mg / L tetracycline, which shows that it has a good tolerance to high concentration of tetracycline.

[0033] 2) Detect the tetracycline concentrat...

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Abstract

The invention belongs to the technical field of agricultural microbiology, and in particular discloses insect stratiomyiid intestinal tract derived serratia marcescens BSFL06 and application thereof. The serratia marcescens is serratia marcescens BSFL06, and the preservation number is CCTCC NO:M 2017073. The serratia marcescens BSFL06 provided by the invention can effectively degrade antibiotic tetracycline, enhances the tetracycline resisting and degrading capabilities of the stratiomyiid in the material transformation process, has bacteriostatic activity on harmful bacteria staphylococcus aureus (S.Aureus) and scherichia coli (E.coli), is stratiomyiid intestinal tract probiotics and can be used for promoting the stratiomyiid to perform transformation on tetracycline polluted waste.

Description

technical field [0001] The invention belongs to the technical field of agricultural microbiology, and in particular relates to Serratia marcescens BSFL-6 derived from the intestinal tract of insect soldier flies and its application. The Serratia marcescens BSFL-6 is isolated from transformed tetracycline-containing materials The intestinal tract of the soldier fly also relates to the application of the Serratia marcescens and the soldier fly to synergistically degrade tetracycline, and also involves the application of the Serratia marcescens to inhibit the growth of harmful bacteria Staphylococcus aureus and Escherichia coli. Background technique [0002] With the rapid development of intensive modern livestock and poultry farming, the use of antibiotics in its production process is increasing. High doses of antibiotics can be used to treat various diseases, while low doses can be used to promote the growth and development of livestock and poultry. To increase production, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02A61K35/74A61P31/04A62D101/28C12R1/43
CPCA61K35/74A62D3/02A62D2101/28C12N1/20C12N1/205C12R2001/43Y02A50/30
Inventor 蔡珉敏张吉斌胡芮绮马世腾刘念伍相佶张可李武郑龙玉李庆喻子牛
Owner HUAZHONG AGRI UNIV
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