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Tissue-culture rapid propagation method for moringa

A technology of tissue culture and Moringa oleifera, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of not being universally applicable, low survival rate of regenerated plants, complicated technical requirements, etc. Multiplication, beneficial to factory operation, and high reproduction coefficient

Inactive Publication Date: 2017-08-08
YUNNAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some scholars have carried out research to Moringa oleifera tissue culture technology in the past, but the application of research result in Moringa oleifera seedling production is not general yet, traces it to its reason, technical requirement is too complicated in existing tissue culture research, does not have universal applicability, the organization There are no effective solutions to the problems encountered in the cultivation process, and the low survival rate of regenerated plants is the main factor. Moringa tissue culture technology still has a lot of room for development

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Disinfection and germination of Moringa oleifera seeds: Disinfect the peeled seed embryos on an ultra-clean workbench immediately after removing the shell. % mercuric chloride solution, shake and soak for 20min, and finally wash 6 times with sterile water; the germination temperature of Moringa oleifera seeds is 25~28℃; Cotyledons, using the epicotyl as the material for adventitious bud induction;

[0016] (2) Adventitious bud induction: Inoculate the epicotyls obtained in step (1) into adventitious bud induction medium: MS+0.3mg / L6-BA+0.04mg / L NAA+20.0g / L sucrose+2.75 g / L Agar, pH 5.8~5.9, and cultured at 25±2°C under natural light conditions to induce adventitious buds, when the induced cluster buds or single buds grow to 3~5cm, the cut growth is more consistent, healthy and not Single buds with or with a small amount of callus;

[0017] (3) Subculture: inoculate the single bud from step (2) into the subculture medium: MS+0.4 mg / L 6-BA+0.03 mg / L NAA+20.0 g / L suc...

Embodiment 2

[0020] (1) Disinfection and germination of Moringa oleifera seeds: Disinfect the peeled seed embryos on the ultra-clean workbench immediately after removing the shell. % mercuric chloride solution, shake and soak for 20min, and finally wash 6 times with sterile water; the germination temperature of Moringa oleifera seeds is 25~28℃; Cotyledons, using the epicotyl as the material for adventitious bud induction;

[0021] (2) Adventitious bud induction: inoculate the epicotyls obtained in step (1) into adventitious bud induction medium: MS+0.1mg / L6-BA+0.01mg / L NAA+20.0g / L sucrose+2.75 g / L Agar, pH 5.8~5.9, and cultured at 25±2°C under natural light conditions to induce adventitious buds, when the induced cluster buds or single buds grow to 3~5cm, the cut growth is more consistent, healthy and not Single buds with or with a small amount of callus;

[0022] (3) Subculture: Inoculate the single bud from step (2) into the subculture medium: MS+0.1 mg / L 6-BA+0.01 mg / L NAA+20.0 g / L su...

Embodiment 3

[0025](1) Disinfection and germination of Moringa oleifera seeds: Disinfect the peeled seed embryos on the ultra-clean workbench immediately after removing the shell. % mercuric chloride solution, shake and soak for 20min, and finally wash 6 times with sterile water; the germination temperature of Moringa oleifera seeds is 25~28℃; Cotyledons, using the epicotyl as the material for adventitious bud induction;

[0026] (2) Adventitious bud induction: Inoculate the epicotyls obtained in step (1) into adventitious bud induction medium: MS+0.5mg / L6-BA+0.05mg / L NAA+20.0g / L sucrose+2.75 g / L Agar, pH 5.8~5.9, and cultured at 25±2°C under natural light conditions to induce adventitious buds, when the induced cluster buds or single buds grow to 3~5cm, the cut growth is more consistent, healthy and not Single buds with or with a small amount of callus;

[0027] (3) Subculture: inoculate the single bud from step (2) into the subculture medium: MS+0.5 mg / L 6-BA+0.05 mg / L NAA+20.0 g / L suc...

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Abstract

The invention provides a tissue-culture rapid propagation method for moringa. The method comprises the steps of disinfection and germination of moringa seeds, adventitious bud induction, subculture propagation and rooting culture, thereby completing the tissue-culture rapid propagation of the moringa. According to the method, the rapid propagation of the moringa is carried out in an organ propagation mode without a dedifferentiation process, so that chromosomal variation caused in the dedifferentiation process is avoided, and the stability of heredity is high; by a bud propagation mode, the propagation coefficient is large, the propagation speed is high, and thus, relatively high requirements of the market on moringa seedlings can be met. According to the method, development and innovation are carried out on the basis of tests of predecessors, and adopted culture medium types and culture conditions are more beneficial to the induction and propagation of adventitious buds of the moringa and also are more beneficial to factory operation.

Description

technical field [0001] The invention relates to a tissue culture rapid propagation method of Moringa oleifera, belonging to the technical field of seedling cultivation. Background technique [0002] At present, there are few domestic literatures about the rapid propagation of Moringa oleifera. Li Guoyun et al. used the top buds and young side buds of Moringa oleifera seedlings in the same year as propagation materials, and used bud organs to culture in vitro for rapid propagation. They obtained a large number of clustered buds without callus, and strong seedlings took root. Field transplantation was successful, the first time The tissue culture technique of Moringa oleifera was explored. 2007 was a year of research on Moringa oleifera tissue culture technology. Ma Chongjian et al. used seed germination to induce aseptic seedlings, and through the callus generation pathway, induced Moringa oleifera callus, induction and proliferation of clustered buds, and rooting. The form...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 赵一鹤吴义军施蕊张静美夏菁
Owner YUNNAN ACAD OF FORESTRY
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