Plural gel containing phycoerythrin, preparation method and application

A phycoerythrin and composite gel technology, applied in the chemical field, can solve the problems of low photoelectric conversion efficiency of dye-sensitized cells, protein denaturation, and application difficulties, and achieve simple separation and purification, low cost, and high sample purity. Effect

Active Publication Date: 2017-08-08
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the phycoerythrin dye-sensitized layer mostly exists in liquid form, it is difficult to apply
However, if the solid phycoerythrin biological dye-sensitized

Method used

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  • Plural gel containing phycoerythrin, preparation method and application
  • Plural gel containing phycoerythrin, preparation method and application
  • Plural gel containing phycoerythrin, preparation method and application

Examples

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Embodiment 1

[0038]A preparation method of phycoerythrin / collagen / carboxylated carbon nanotube / polyacrylamide composite gel, comprising the steps of:

[0039] (1) Extracting collagen and preparing an aqueous collagen solution with a concentration of 5 mg / mL;

[0040] Take the fish skin and soak it in NaOH aqueous solution with a concentration of 0.2mol / L for 24 hours to remove non-collagen components. After washing to neutrality, add acetic acid solution with a concentration of 0.6mol / L to homogenate, extract with magnetic stirring at 4°C for 2 days, and centrifuge at 12000g for 30 minutes , add NaCl to the supernatant, centrifuge, collect and dissolve the precipitate in 0.5 mol / L acetic acid solution, dialyze with 0.1 mol / L acetic acid solution overnight, then dialyze with distilled water for 1 day, and freeze-dry to obtain acid-soluble fish collagen. Take acidic fish collagen and dissolve it in deionized water, adjust the pH to 6.0 with NaOH, and adjust the concentration of fish collagen...

Embodiment 2

[0050] A preparation method of phycoerythrin / collagen / carboxylated carbon nanotube / polyacrylamide composite gel, comprising the steps of:

[0051] (1) Extracting collagen and preparing an aqueous collagen solution with a concentration of 6 mg / mL;

[0052] Take the fish skin and soak it in 0.3mol / L NaOH aqueous solution for 14 hours to remove non-collagen components, wash it until neutral, add 0.4mol / L acetic acid solution for homogenization, extract with magnetic stirring at 4°C for 1 day, and centrifuge at 12000g for 30 minutes , add NaCl to the supernatant, centrifuge, collect and dissolve the precipitate in 0.6 mol / L acetic acid solution, dialyze with 0.1 mol / L acetic acid solution overnight, then dialyze with distilled water for 1 day, and freeze-dry to obtain acid-soluble fish collagen. Dissolve the acidic fish collagen into deionized water, adjust the pH to 6.0 with NaOH, and adjust the fish collagen concentration to 6 mg / ml.

[0053] (2) preparation concentration is th...

Embodiment 3

[0063] A preparation method of phycoerythrin / collagen / carboxylated carbon nanotube / polyacrylamide composite gel, comprising the steps of:

[0064] (1) Extracting collagen and preparing an aqueous collagen solution with a concentration of 7 mg / mL;

[0065] Take the fish skin and soak it in 0.1mol / L NaOH aqueous solution for 48 hours to remove the non-collagen components. After washing to neutrality, add 0.5mol / L acetic acid solution for homogenization, extract with magnetic stirring at 4°C for 2 days, and centrifuge at 12000g for 30 minutes , add NaCl to the supernatant, centrifuge, collect and dissolve the precipitate in 0.8 mol / L acetic acid solution, dialyze with 0.1 mol / L acetic acid solution overnight, then dialyze with distilled water for 2 days, and freeze-dry to obtain acid-soluble fish collagen. Dissolve the acidic fish collagen into deionized water, adjust the pH to 6.0 with NaOH, and adjust the fish collagen concentration to 7 mg / ml.

[0066] (2) preparation concent...

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Abstract

The invention discloses plural gel containing phycoerythrin, a preparation method and application. The plural gel is phycoerythrin/collagen/carboxylic carbon nano tube/polyacrylamide plural gel. According to the plural gel disclosed by the invention, the phycoerythrin is prepared into a form of the phycoerythrin/collagen/carboxylic carbon nano tube/polyacrylamide plural gel through an appropriate preparation method, the phycoerythrin can be applied to sensitization batteries, and photoelectric converting efficiency is improved.

Description

technical field [0001] The invention belongs to the field of chemistry, and specifically relates to a composite gel containing phycoerythrin, a preparation method and application thereof, more specifically a composite gel of phycoerythrin / collagen / carboxylated carbon nanotube / polyacrylamide, Preparation method and application. Background technique [0002] Solar energy is the most abundant sustainable energy resource, and the surface of the earth receives about 4×10 24 J Solar. Photosynthesis in nature has been a typical application of solar energy for more than 350 million years. Biological processes are the largest-scale conversions of photon energy into Gibbs free energy. Since the 1990s, the invention of solar cells has promoted the research and application of scientists using biological macromolecules to assemble dye-sensitized solar cells. The characteristics of low cost, environmental friendliness and simple fabrication make dye-sensitized cells have good applicat...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L33/26C08L89/00C08K9/04C08K7/24H01G9/20
CPCY02E10/542C08J3/075C08L33/26C08L2205/025C08L2205/03H01G9/2059C08L89/00C08K9/04C08K7/24
Inventor 蒲洋李文军唐志红秦松
Owner LUDONG UNIVERSITY
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