Lymphocyte culture device

Abstract

The invention discloses a lymphocyte culture device which comprises a cup body and an upper cover. An external thread section is arranged on an outer wall of the upper end of the cup body, the upper cover is divided into a column face section and a top wall, an internal thread is arranged on the inner surface of the column face section, the internal thread is matched with the external thread section of the cup body, the upper cover is arranged at the upper end of the cup body in a sleeving mode, a silica gel sealing pad is arranged between the top wall of the upper cover and a cup opening at the upper end of the cup body, the whole cup opening is covered by the sealing pad, and a pin hole penetrating through the top wall is formed in the middle of the top wall.

Description

technical field [0001] The invention relates to the technical field of disposable consumables used in hospital laboratory tests, in particular to a lymphocyte culture device. Background technique [0002] The culture of human peripheral blood lymphocytes is a common test method for human lymphocytes. It is often used in major hospitals and research institutes in China. The whole culture process includes the following steps: [0003] (1) Sampling and inoculation: Disinfect the bottle cap with 2.5% iodine and 75% alcohol, burn it with an alcohol lamp flame, extract 0.5-1ml of peripheral blood from the patient under sterile conditions, add about 20 drops to each bottle, shake it evenly, and try to Avoid contact of the culture with the bottle cap; [0004] (2) Culture: culture in a 36°C incubator for 72 hours, lightly shake once every 12 hours to promote cell growth and proliferation; [0005] (3) Inhibition of division: Add colchicine about 2 hours before harvesting, the fina...

AI Technical Summary

Problems solved by technology

[0012] In the above culture process, the culture bottle (also known as the culture device) is used as a holding container during the whole test process. In the first step, peripheral blood should be opened and disinfected, and in the third step, colchicine should be added. , for this culture bottle, every time it is opened, there will be the possibility of being polluted by the outside world. The culture bottle (also known as the culture device) in the prior art is composed of a bottle body with a threaded upper cover on it, basically Every time you add something to it, you have to open the lid. The cultured things inside are easily polluted by the outside world, so it needs to be improved.

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