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Citrobacter sp.JPG1 for heavy metal copper ion removal and application thereof

A technology of citric acid bacteria and heavy metals, applied in the field of environmental microorganisms, can solve the problems of high difficulty coefficient of follow-up treatment, large amount of drug usage, cumbersome process, etc., and achieve good application prospects

Inactive Publication Date: 2017-08-08
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical methods such as electrodialysis and chemical precipitation have disadvantages such as cumbersome process, large amount of drugs used, large amount of sediment, and high difficulty in subsequent treatment; physical methods such as activated carbon adsorption, ion exchange and other technologies have poor stability and high operating costs.

Method used

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  • Citrobacter sp.JPG1 for heavy metal copper ion removal and application thereof
  • Citrobacter sp.JPG1 for heavy metal copper ion removal and application thereof
  • Citrobacter sp.JPG1 for heavy metal copper ion removal and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Isolation, purification and identification of Citrobacter sp.JPG1.

[0028] The LB liquid medium used in the isolation process of Citrobacter sp.JPG1 consisted of: 2g peptone, 1g yeast powder, 2g NaCl, 200mL water, pH 7.0-7.5.

[0029] The composition of the LB solid medium is as follows: 16 g / L of agar is added to the above-mentioned liquid medium.

[0030] Citrobacter sp.JPG1 strain was isolated from the tailings residue of a tailing pond in Jilin Province. The specific enrichment, separation and purification process are as follows:

[0031] Inoculate 1g of tailings slag into physiological saline (filling volume: 20ml / 100ml Erlenmeyer flask), shake at 180rpm for 24h. Pipette 1ml of the suspension into a centrifuge tube, add the supernatant to 99ml of freshly sterilized LB liquid medium, and culture on a shaking table at 30°C and 180r / min for 24h. After the expanded culture, the bacterial solution was inoculated sequentially to the Cu-containing 2+ The LB...

Embodiment 2

[0039] Embodiment 2. The growth curve of Citrobacter sp.JPG1 strain and its effect on Cu 2+ tolerance test.

[0040] In order to study the growth law of Citrobacter sp.JPG1, the preserved strains were activated to make a bacterial suspension and inoculated into LB medium according to the inoculum amount of 1%. Bed cultured for 96 hours, then samples were taken at 0, 3, 6, 9, 12, 18, 24, 32, 36, and 48 hours to measure the OD of the bacterial solution 600 value, draw the growth curve of Citrobacter sp.JPG1. See the growth curve of Citrobacter sp.JPG1 figure 2 . The growth period of Citrobacter sp.JPG1 is generally 48h. The adaptation period is about 0 to 9 hours; 9 to 24 hours is the logarithmic growth period; after 24 hours, it enters the stable period.

[0041] To study the effect of Citrobacter sp.JPG1 on Cu 2+ The degree of tolerance of the strain was studied in different Cu 2+ Growth regulation in the case of concentration inhibition. The preserved bacterial suspe...

Embodiment 3

[0042] Example 3. Citrobacter sp.JPG1 to Cu in copper-containing wastewater 2+ Biosorption

[0043] The adsorption of heavy metals by living bacterial cells includes two stages: the first stage is the adsorption of heavy metals on the bacterial surface, and the second stage is the process in which heavy metal ions enter cells directly through ion channels on the cell surface or combine with enzymes, and accumulate in the cells . In order to study the effect of Citrobacter sp.JPG1 living cells on Cu in copper-containing wastewater 2+ The adsorption effect, take living cells (1.0g / L), add artificially prepared copper-containing wastewater (Cu 2+ Concentrations were 50mg / L and 100mg / L), after oscillating and adsorbing on a shaking table for 24h, centrifuge and sample (8000r / min, 5min), measure supernatant concentration, and calculate adsorption capacity. Wash 2 times with sterile water, collect the bacteria, add 10ml 5mM EDTA, shake for 30min, centrifuge again, collect the bac...

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Abstract

The invention provides a heavy-metal-copper-resistant bacterial strain and application thereof. The classification and name of the heavy-metal-copper-resistant bacterial strain is Citrobacter sp.JPG1, and has the preservation register number of CGMCC (China General Microbiological Culture Collection Center) No.13480 in CGMCC. The strain is obtained through separation, screening, identification and mutagenesis. The bacteria can endure and adsorb heavy metal copper, and can be used for microbial treatment of heavy-metal-copper-containing wastewater.

Description

[0001] Summary [0002] The invention belongs to the technical field of environmental microorganisms. It specifically relates to a strain of Citrobacter sp. and its application in the removal of heavy metal copper ion-containing wastewater. The bacterial strain is Citrobacter sp. JPG1, and its preservation number in the General Microbiology Center of China Committee for Culture Collection of Microorganisms is CGMCC No. 13480. The strain has the ability to tolerate heavy metal copper and can be used for the treatment of copper-containing wastewater. technical field [0003] The invention belongs to the technical field of environmental microorganisms. It specifically relates to a strain of Citrobacter sp. JPG1 for removing heavy metal copper ions and its application. Background technique [0004] Copper is an essential element for biological growth, but can be toxic in high concentrations. When the copper content in water exceeds 0.01mg / L, it will not only significantly in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/20
CPCC02F3/34C02F2101/20C12N1/205C12R2001/01
Inventor 王小雨黄宁毛娟
Owner NORTHEAST NORMAL UNIVERSITY
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