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Cisplatin-loaded hydrogel drug delivery system and preparation method thereof

A hydrogel and cisplatin technology, applied in liquid delivery, antitumor drugs, drug combination and other directions, can solve problems such as drug failure and clinical application limitations, and achieve pain relief, stable drug loading system, and good biocompatibility. Effect

Inactive Publication Date: 2017-08-11
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cisplatin is mostly used as an injection in clinical practice, but because cisplatin is easily combined with protein substances in the body, resulting in drug failure, and the long half-life of cisplatin makes it have long-term biological toxicity, so its clinical application is limited

Method used

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  • Cisplatin-loaded hydrogel drug delivery system and preparation method thereof
  • Cisplatin-loaded hydrogel drug delivery system and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1) Weigh 10.000 g of pullulan and dissolve it in 400 mL of double-distilled water, and stir it with magnetic force to fully dissolve it.

[0014] 2) Add 10 mL of 232 mg / mL potassium periodate solution into the solution of 1) and react for 1.5 h.

[0015] 3) Add 0.72 mL glycerol to the solution in 2) and react for 15 min to terminate the oxidation reaction.

[0016] 4) Put solution 3) in a dialysis bag for dialysis at 4°C for 3 days, during which time the water was changed every 6-8 hours.

[0017] 5) The dialyzed solution 4) was vacuum freeze-dried for 3 days to obtain pure oxidized pullulan.

[0018] 6) Weigh 1.000 g of oxidized pullulan solid, add 20 mL of double distilled water and dissolve at 37 °C.

[0019] 7) Add 30 mg of cisplatin to solution 6), and fully dissolve at 37°C.

[0020] 8) Add 250 μL of 0.5 g / mL ε-polylysine solution to solution 7), and mix thoroughly.

[0021] 9) Add 30 μL of 0.26 g / mL branched polyethyleneimine solution to the above solution 8)...

Embodiment 2

[0024] 1) Weigh 10.000 g pullulan polysaccharide and dissolve it in 400 mL double distilled water, and stir it with magnetic force to fully dissolve it.

[0025] 2) 2) Add 10 mL of 232 mg / mL potassium periodate solution into the solution of 1) and react for 1.5 h.

[0026] 3) Add 0.72 mL glycerol to 2) and react for 15 min to terminate the oxidation reaction.

[0027] 4) Place the solution 3) in a dialysis bag at 4°C for 3 days, during which time the water is changed every 6-8 hours.

[0028] 5) The dialyzed solution 4) was vacuum freeze-dried for 3 days to obtain pure oxidized pullulan solid.

[0029] 6) Weigh 1.000 g of oxidized pullulan solid, add 20 mL of double distilled water and dissolve at 37 °C.

[0030] 7) Add 30 mg of cisplatin to solution 6), and fully dissolve at 37°C.

[0031] 8) Add 350 μL of 0.5 g / mL ε-polylysine solution to solution 7), and mix thoroughly.

[0032] 9) Add 30 μL of 0.26 g / mL branched polyethyleneimine solution to the above solution 8), and ...

Embodiment 3

[0035] 1) Weigh 10.000 g pullulan polysaccharide and dissolve it in 400 mL double distilled water, and stir it with magnetic force to fully dissolve it.

[0036] 2) Add 10 mL of 232 mg / mL potassium periodate solution into the solution of 1) and react for 1.5 h.

[0037] 3) Add 0.72 mL glycerol to 2) and react for 15 min to terminate the oxidation reaction.

[0038] 4) Put solution 3) in a dialysis bag for dialysis at 4°C for 3 days, during which time the water was changed every 6-8 hours.

[0039] 5) The dialyzed solution 4) was vacuum freeze-dried for 3 days to obtain pure oxidized pullulan solid.

[0040] 6) Weigh 1.000 g of oxidized pullulan solid, add 20 mL of double distilled water and dissolve at 37 °C.

[0041] 7) Add 30 mg of cisplatin to solution 6), and fully dissolve at 37°C.

[0042] 8) Add 450 μL of 0.5 g / mL ε-polylysine solution to solution 7), and mix thoroughly.

[0043] 9) Add 20 μL of 0.26 g / mL branched polyethyleneimine solution to the above solution 8),...

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Abstract

The invention discloses a cisplatin-loaded hydrogel drug delivery system and a preparation method thereof. The preparation method comprises the following steps: oxidizing pullulan so as to obtain oxidized pullulan rich in aldehyde group; and with the oxidized pullulan, [epsilon]-polylysine rich in amino group, low-molecular weight grafted polyethyleneimine and a broad-spectrum anti-cancer drug, namely cisplatin, serving as raw materials, conducting a Schiff base reaction in a water solution, so that a dynamic imine linkage cross-linked network is formed, and the hydrogel drug delivery system is prepared. The preparation method provided by the invention is short in preparation time and simple to operate; the cisplatin is loaded into a hydrogel network in two modes, namely dynamic imine linkage cross-linking and adsorption, so that the drug delivery system is more stable; moreover, the cisplatin undergoes controlled-release, so that a purpose of effectively controlling toxicity of drugs in a tumor treatment process is achieved. In addition, the hydrogel is excellent in biocompatibility, self-healing performance and syringeability; therefore, the hydrogel drug delivery system, instead of being implanted through a surgical operation, can be directly injected to peritumoral tissues or into cancers, so that patient's pain is greatly relieved.

Description

technical field [0001] The invention belongs to the field of polymer drug carriers, and in particular relates to a cisplatin-loaded hydrogel drug-loading system and a preparation method thereof. Background technique [0002] Platinum drugs have developed rapidly in the field of anti-cancer because of their broad anti-cancer spectrum, and cisplatin has become one of the main drugs in cancer chemotherapy. Cisplatin is mostly used as an injection in clinical practice, but because cisplatin is easily combined with protein substances in the body, resulting in drug failure, and the long half-life of cisplatin makes it have long-term biological toxicity, so its clinical application is limited. In order to solve the strong toxic and side effects of direct injection, in recent years, researchers have combined conventional chemotherapy drugs with polymer drug carriers to form a polymer drug reservoir with local slow-release effect. Perform controlled release to ensure safety and effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/36A61K47/34A61K33/24A61P35/00
Inventor 程翠张秀丽孟亚彬章志鸿张其清伍久林
Owner FUZHOU UNIV
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