Cisplatin-loaded hydrogel drug delivery system and preparation method thereof
A hydrogel and cisplatin technology, applied in liquid delivery, antitumor drugs, drug combination and other directions, can solve problems such as drug failure and clinical application limitations, and achieve pain relief, stable drug loading system, and good biocompatibility. Effect
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Embodiment 1
[0013] 1) Weigh 10.000 g of pullulan and dissolve it in 400 mL of double-distilled water, and stir it with magnetic force to fully dissolve it.
[0014] 2) Add 10 mL of 232 mg / mL potassium periodate solution into the solution of 1) and react for 1.5 h.
[0015] 3) Add 0.72 mL glycerol to the solution in 2) and react for 15 min to terminate the oxidation reaction.
[0016] 4) Put solution 3) in a dialysis bag for dialysis at 4°C for 3 days, during which time the water was changed every 6-8 hours.
[0017] 5) The dialyzed solution 4) was vacuum freeze-dried for 3 days to obtain pure oxidized pullulan.
[0018] 6) Weigh 1.000 g of oxidized pullulan solid, add 20 mL of double distilled water and dissolve at 37 °C.
[0019] 7) Add 30 mg of cisplatin to solution 6), and fully dissolve at 37°C.
[0020] 8) Add 250 μL of 0.5 g / mL ε-polylysine solution to solution 7), and mix thoroughly.
[0021] 9) Add 30 μL of 0.26 g / mL branched polyethyleneimine solution to the above solution 8)...
Embodiment 2
[0024] 1) Weigh 10.000 g pullulan polysaccharide and dissolve it in 400 mL double distilled water, and stir it with magnetic force to fully dissolve it.
[0025] 2) 2) Add 10 mL of 232 mg / mL potassium periodate solution into the solution of 1) and react for 1.5 h.
[0026] 3) Add 0.72 mL glycerol to 2) and react for 15 min to terminate the oxidation reaction.
[0027] 4) Place the solution 3) in a dialysis bag at 4°C for 3 days, during which time the water is changed every 6-8 hours.
[0028] 5) The dialyzed solution 4) was vacuum freeze-dried for 3 days to obtain pure oxidized pullulan solid.
[0029] 6) Weigh 1.000 g of oxidized pullulan solid, add 20 mL of double distilled water and dissolve at 37 °C.
[0030] 7) Add 30 mg of cisplatin to solution 6), and fully dissolve at 37°C.
[0031] 8) Add 350 μL of 0.5 g / mL ε-polylysine solution to solution 7), and mix thoroughly.
[0032] 9) Add 30 μL of 0.26 g / mL branched polyethyleneimine solution to the above solution 8), and ...
Embodiment 3
[0035] 1) Weigh 10.000 g pullulan polysaccharide and dissolve it in 400 mL double distilled water, and stir it with magnetic force to fully dissolve it.
[0036] 2) Add 10 mL of 232 mg / mL potassium periodate solution into the solution of 1) and react for 1.5 h.
[0037] 3) Add 0.72 mL glycerol to 2) and react for 15 min to terminate the oxidation reaction.
[0038] 4) Put solution 3) in a dialysis bag for dialysis at 4°C for 3 days, during which time the water was changed every 6-8 hours.
[0039] 5) The dialyzed solution 4) was vacuum freeze-dried for 3 days to obtain pure oxidized pullulan solid.
[0040] 6) Weigh 1.000 g of oxidized pullulan solid, add 20 mL of double distilled water and dissolve at 37 °C.
[0041] 7) Add 30 mg of cisplatin to solution 6), and fully dissolve at 37°C.
[0042] 8) Add 450 μL of 0.5 g / mL ε-polylysine solution to solution 7), and mix thoroughly.
[0043] 9) Add 20 μL of 0.26 g / mL branched polyethyleneimine solution to the above solution 8),...
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