Applications of firmicute probiotics in preventing and/or treating diabetes and relevant diseases
A technology of probiotics and wall fungi, applied in the field of microbiology, can solve the problems of inability to explain the pathogenic mechanism of type II diabetes and have small side effects
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Embodiment 1
[0112] Embodiment 1 Food composition containing Firmicutes probiotics (such as Lactobacillus oralis, Megasphaera escherichia, or a combination thereof)
[0113] The ratio of raw materials is shown in Table 1.
[0114] Table 1 food composition formula
[0115] raw material Mass percentage (%) Bacterial components 0.5 milk 90.0 white sugar 9.5
[0116] The bacterial components in formulas 1-6 are single bacterial components, which respectively contain Lactobacillus oris DSM 4864, Lactobacillus oris DSM 4865, Lactobacillus oris DSM 4866, Megasphaera elsdenii DSM 20460, Megasphaera elsdenii ATCC 17752, and Megasphaera elsdenii ATCC17753.
[0117] The bacteria component in Formula 7 is a mixture of any two of the above-mentioned 6 kinds of bacteria (the weight ratio is 1:1).
[0118] Mix milk and sugar according to the above formula ratio, stir until completely mixed, preheat, homogenize with 20Mpa pressure, sterilize at about 90°C for 5-10 minut...
Embodiment 2
[0120] A pharmaceutical composition of mectenoid probiotics (such as Lactobacillus oralis, Megasphaera escherichia, or a combination thereof)
[0121] The ratio of raw materials is shown in Table 2.
[0122] Table 2 Pharmaceutical composition formula
[0123]
[0124]
[0125] The bacterial components in formulas 1-6 are single bacterial components, which respectively contain Lactobacillus oris DSM 4864, Lactobacillus oris DSM 4865, Lactobacillus oris DSM 4866, Megasphaera elsdenii DSM 20460, Megasphaera elsdenii ATCC 17752, and Megasphaera elsdenii ATCC17753.
[0126] The bacteria component in Formula 7 is a mixture of any two of the above-mentioned 6 kinds of bacteria (the weight ratio is 1:1).
[0127] Mix lactose, yeast powder, and peptone evenly with purified water according to the proportion, preheat to 60-65°C, homogenize with 20Mpa pressure, sterilize at about 90°C for 20-30 minutes, cool to 36-38°C, add the bacterial component ( 1-50×10 6 cfu / mL), fermented at ...
Embodiment 3
[0128] Embodiment 3 animal experiment verification
[0129] Experimental Materials:
[0130] Mice: C57BL / 6J male mice (4 weeks old, purchased from the Experimental Animal Center of Sun Yat-sen University) were purchased, and the mice were grown in the same environment and fed with the same food.
[0131] The inventor obtained 6 strains of Firmicutes probiotics from the depository institution, and preserved them in Shenzhen Huada Gene Research Institute. Meanwhile, select Lactobacillus reuteri (Lactobacillus reuteri GMNL-89), purchased from China Center for Type Culture Collection (CCTCC), preservation number is CCTCC NO: M207154, as a control group (LR group), in MRS culture medium, Incubate at 37°C for 24-48h.
[0132] Among them, the source information of the above-mentioned 6 strains of Firmicutes probiotics is shown in Table 3. Bacteria 1-3 were cultured anaerobically at 37° C. for 24-48 h in MRS medium (DSMZ Medium 11). Bacteria 4-6 were cultured anaerobically at 37°C...
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