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Applications of firmicute probiotics in preventing and/or treating diabetes and relevant diseases

A technology of probiotics and wall fungi, applied in the field of microbiology, can solve the problems of inability to explain the pathogenic mechanism of type II diabetes and have small side effects

Inactive Publication Date: 2017-08-11
SHENZHEN HUADA GENE INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many studies on these aspects, but none of them can explain the occurrence and pathogenesis of type II diabetes very well
[0005] At present, there is no effective method and medicine for preventing and / or treating diabetes and its related diseases with little side effects

Method used

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  • Applications of firmicute probiotics in preventing and/or treating diabetes and relevant diseases
  • Applications of firmicute probiotics in preventing and/or treating diabetes and relevant diseases
  • Applications of firmicute probiotics in preventing and/or treating diabetes and relevant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0112] Embodiment 1 Food composition containing Firmicutes probiotics (such as Lactobacillus oralis, Megasphaera escherichia, or a combination thereof)

[0113] The ratio of raw materials is shown in Table 1.

[0114] Table 1 food composition formula

[0115] raw material Mass percentage (%) Bacterial components 0.5 milk 90.0 white sugar 9.5

[0116] The bacterial components in formulas 1-6 are single bacterial components, which respectively contain Lactobacillus oris DSM 4864, Lactobacillus oris DSM 4865, Lactobacillus oris DSM 4866, Megasphaera elsdenii DSM 20460, Megasphaera elsdenii ATCC 17752, and Megasphaera elsdenii ATCC17753.

[0117] The bacteria component in Formula 7 is a mixture of any two of the above-mentioned 6 kinds of bacteria (the weight ratio is 1:1).

[0118] Mix milk and sugar according to the above formula ratio, stir until completely mixed, preheat, homogenize with 20Mpa pressure, sterilize at about 90°C for 5-10 minut...

Embodiment 2

[0120] A pharmaceutical composition of mectenoid probiotics (such as Lactobacillus oralis, Megasphaera escherichia, or a combination thereof)

[0121] The ratio of raw materials is shown in Table 2.

[0122] Table 2 Pharmaceutical composition formula

[0123]

[0124]

[0125] The bacterial components in formulas 1-6 are single bacterial components, which respectively contain Lactobacillus oris DSM 4864, Lactobacillus oris DSM 4865, Lactobacillus oris DSM 4866, Megasphaera elsdenii DSM 20460, Megasphaera elsdenii ATCC 17752, and Megasphaera elsdenii ATCC17753.

[0126] The bacteria component in Formula 7 is a mixture of any two of the above-mentioned 6 kinds of bacteria (the weight ratio is 1:1).

[0127] Mix lactose, yeast powder, and peptone evenly with purified water according to the proportion, preheat to 60-65°C, homogenize with 20Mpa pressure, sterilize at about 90°C for 20-30 minutes, cool to 36-38°C, add the bacterial component ( 1-50×10 6 cfu / mL), fermented at ...

Embodiment 3

[0128] Embodiment 3 animal experiment verification

[0129] Experimental Materials:

[0130] Mice: C57BL / 6J male mice (4 weeks old, purchased from the Experimental Animal Center of Sun Yat-sen University) were purchased, and the mice were grown in the same environment and fed with the same food.

[0131] The inventor obtained 6 strains of Firmicutes probiotics from the depository institution, and preserved them in Shenzhen Huada Gene Research Institute. Meanwhile, select Lactobacillus reuteri (Lactobacillus reuteri GMNL-89), purchased from China Center for Type Culture Collection (CCTCC), preservation number is CCTCC NO: M207154, as a control group (LR group), in MRS culture medium, Incubate at 37°C for 24-48h.

[0132] Among them, the source information of the above-mentioned 6 strains of Firmicutes probiotics is shown in Table 3. Bacteria 1-3 were cultured anaerobically at 37° C. for 24-48 h in MRS medium (DSMZ Medium 11). Bacteria 4-6 were cultured anaerobically at 37°C...

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Abstract

The invention discloses applications of firmicute probiotics in preventing and / or treating diabetes and relevant diseases, specifically, the firmicute probiotics are used or preparing a composition or a preparation. The composition or the preparation has the following one or more applications: (1) preventing and / or treating diabetes; (b) preventing and / or treating cardiovascular diseases; and / or (c) preventing and / or treating obesity. The firmicute probiotics can obviously inhibit the weight increase, reduce the serum glucose level, reduce the blood lipid level, improve the insulin resistance and / or reduce the glycosylated hemoglobin level, and thus the symptoms of diabetes and relevant diseases can be effectively alleviated.

Description

technical field [0001] The invention belongs to the field of microbiology. Specifically, the invention relates to the application of Firmicutes probiotics in the prevention and / or treatment of diabetes and related diseases, and also relates to a composition containing Firmicutes probiotics and its application. Background technique [0002] There are a large number of symbiotic microorganisms in the human body, most of which live in the human intestinal tract, and the number exceeds 1000 trillion (10 14 Order of magnitude), more than 10 times the total number of human cells. During the long evolutionary process, intestinal microbes have reached a good cooperation with human beings, and play a vital role in the nutrition, metabolism and immunity of the human body. Many researchers regard the human intestinal microbiome as more It is an "organ" of the human body, or the second genome of the human body, and the massive genetic information contained in it is closely related to h...

Claims

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Application Information

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IPC IPC(8): A61K35/741A61P3/10A61P9/00A61P3/04A61P3/06A23L33/135A23L2/52A23K10/18
CPCA23L2/52A61K35/741A23V2002/00A23V2400/11A23V2400/113A23V2400/51A61K2300/00A23V2200/30A23V2200/328
Inventor 冯强宁光张东亚顾燕云揭著业王卫庆
Owner SHENZHEN HUADA GENE INST
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