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Method for culturing bletilla striata seeds into seedlings

A cultivation method and seed technology, which are applied in the cultivation field of plant seeds and seedlings, can solve the problems of many intermediate production links, low transplanting survival rate, long production cycle, etc., and achieve well-developed root system, high transplanting survival rate, and labor saving cost effect

Inactive Publication Date: 2017-08-18
WENSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This tissue culture breeding system has many intermediate production links, long production cycle, and high labor cost; although the propagation coefficient can be increased through protocorm differentiation and clustered bud proliferation, the tissue culture seedlings are not as good as the seedlings cultivated by this technology. Weak, underdeveloped root system, low transplant survival rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: a kind of cultivation method of Bletilla striata seed cultivation seedling comprises the following steps:

[0021] (1) Seed collection and storage: From September to October, when the bletilla striata capsules matured from light yellow to brown, the mature bletilla striata capsules were collected and stored in a sealed refrigerator at 4°C for later use; the actual storage quantity of the bletilla striata capsules was 120% of the planned sowing amount;

[0022] (2) Disinfection and sterilization: Take seeds with 4-month embryonic age and stored in a refrigerator at 4°C for 1 month as spare materials. Select uncracked Bletilla striata capsules from the spare materials, wash them with running water for 30 minutes, and put them in an ultra-clean working environment. Treat with 75% alcohol on the table for 30 seconds, then soak in 0.1% mercuric chloride solution for 10 minutes, pour off the mercuric chloride solution, rinse 4 times with sterile water, each time...

Embodiment 2

[0026] Embodiment 2: a kind of culture method that bletilla striata seed is cultivated into seedling comprises the following steps:

[0027] (1) Seed collection and storage: From September to October, when the bletilla striata capsules matured from light yellow to brown, the mature bletilla striata capsules were collected and stored in a sealed refrigerator at 4°C for later use; the actual storage quantity of the bletilla striata capsules was 130% of the planned sowing amount;

[0028] (2) Disinfection and sterilization: Take seeds with 4-month embryonic age and stored in a refrigerator at 4°C for 1 month as spare materials. Select uncracked Bletilla striata capsules from the spare materials, wash them with running water for 40 minutes, and put them in the ultra-clean work. Treat with 75% alcohol on the table for 30 seconds, then soak in 0.1% mercuric chloride solution for 15 minutes, pour off the mercuric chloride solution, rinse 5 times with sterile water for 2 minutes each ...

Embodiment 3

[0032] Embodiment 3: a kind of culture method that bletilla striata seed is cultivated into seedling, comprises the following steps:

[0033] (1) Seed collection and storage: From September to October, when the bletilla striata capsules matured from light yellow to brown, the mature bletilla striata capsules were collected and stored in a sealed refrigerator at 4°C for later use; the actual storage quantity of the bletilla striata capsules was 125% of the planned seeding rate;

[0034] (2) Disinfection and sterilization: take seeds with 4-month embryonic age and stored in a refrigerator at 4°C for 1 month as spare materials, select uncracked Bletilla striata capsules from the spare materials, wash them with running water for 35 minutes, and put them in the ultra-clean work. Treat with 75% alcohol on the table for 30 seconds, then soak in 0.1% mercuric chloride solution for 13 minutes, pour off the mercuric chloride solution, rinse 4 times with sterile water, each time for 1 minu...

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PUM

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Abstract

The invention relates to a method for culturing plant seeds into seedlings, in particular to a method for culturing bletilla striata seeds into seedlings. The method comprises the following steps: seed collecting and storing, sterilizing, induction and seedling forming, seedling strengthening and rooting, and seedling hardening and transplanting. By a method for tissue culture of the bletilla striata seeds into seedlings in an induction manner, the method which is high in efficiency and high in rate of survival is provided for tissue culture of bletilla striata, and a large number of robust, safe and high-viability bletilla striata seedlings can be rapidly obtained within a short time.

Description

technical field [0001] The invention relates to a method for cultivating plant seeds into seedlings, in particular to a method for cultivating bletilla striata seeds into seedlings. Background technique [0002] Bletilla striata ( Bletilla striata ( Thunb. ) Reichb. f. ) is a perennial herbaceous plant of the genus Bletilla striata in the family Orchidaceae. The primary pseudobulb is spherical, and it grows to a certain extent before forming a V-shaped massive pseudobulb; the leaves are 4 to 6, narrowly oblong or lanceolate, and 8 to 29 cm long. 1.5-4 cm wide, apex acuminate, base narrowed into a sheath and covering the stem; inflorescence with 3-10 flowers, often unbranched or rarely branched; inflorescence rachis more or less in zigzag shape The bracts are oblong-lanceolate, 2-2.5 cm long; the flowers are large, purple-red or pink; the flowering period is April-May; the fruiting period is August-November; the seeds of Bletilla striata are very small, like powder, witho...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 张铁杨本恒高明海
Owner WENSHAN UNIV
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