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Method for extracting 2-phenylacetamide compound from semen lepidii and application of 2-phenylacetamide compound

A kind of technology of phenylacetamide and Beiting Lizi, applied in the field of medicine, can solve problems such as no public report of 2-phenylacetamide compound, and achieve the effects of novel and unique method, good economic and social benefits, and easy operation

Inactive Publication Date: 2017-08-18
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no public report about the extraction of 2-phenylacetamide compound from Tinglizi and its application in the preparation of estrogen drugs

Method used

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  • Method for extracting 2-phenylacetamide compound from semen lepidii and application of 2-phenylacetamide compound
  • Method for extracting 2-phenylacetamide compound from semen lepidii and application of 2-phenylacetamide compound
  • Method for extracting 2-phenylacetamide compound from semen lepidii and application of 2-phenylacetamide compound

Examples

Experimental program
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Effect test

Embodiment 1

[0010] In the specific implementation of the present invention, a method for extracting 2-phenylacetamide compounds from Tinglizi chinensis is to stir-fry 8 kg of Tinglizi spp., add 10 times the weight of water to extract three times, each time for 1.5h, Combine three extracts, concentrate under reduced pressure to 270g of extract, use 20L ethanol with a volume concentration of 80% to precipitate the extract for 2h, discard the precipitate, centrifugally filter the supernatant, concentrate to 1000ml (no alcohol smell), and put on Dianion HP-20 column (highly porous styrene adsorption / desorption resin), eluted with 20L of water, 60L of 20% ethanol, and 60L of 40% ethanol in sequence, with a flow rate of 20L / 24h, collected the eluted parts of 40% ethanol, and dissolved them in water , centrifugally filtered, concentrated to 35ml, passed through Toyopearl HW-40 chromatographic column, eluted with 3L of water and 3L of 10% methanol aqueous solution in sequence, flow rate 8mL / min, c...

Embodiment 2

[0012] In the specific implementation of the present invention, a method for extracting 2-phenylacetamide compounds from Tinglizi chinensis is to extract 7.5kg of fried Tinglizizi chinensis three times with 10 times the weight of water, each time for 1.5h , combined three extracts, concentrated under reduced pressure to 260g of extract, the extract was precipitated with 19L of ethanol with a volume concentration of 80% for 2h, discarded the precipitate, centrifugally filtered the supernatant, concentrated until it had no alcohol smell, and put it on Dianion HP- 20 columns (highly porous styrene adsorption / desorption resin), eluted with 20L of water, 60L of 20% ethanol, and 60L of 40% ethanol in sequence, with a flow rate of 20L / 24h, collected the eluted parts of 40% ethanol, dissolved them in water, and centrifuged Filter, concentrate to 30ml, pass through Toyopearl HW-40 chromatographic column, elute successively with 3L of water and 3L of 10% methanol aqueous solution at a fl...

Embodiment 3

[0014] In the specific implementation of the present invention, a method for extracting 2-phenylacetamide compounds from Tinglizi chinensis is to extract 8.5kg of fried Tinglizizi chinensis three times with 10 times the weight of water, each time for 1.5h , combined three extracts, concentrated under reduced pressure to 280g extract, the extract was precipitated with 80% ethanol 21L alcohol precipitation for 2h, discarded the precipitate, and the supernatant was centrifugally filtered, concentrated to no alcohol smell (about 1000ml), Put on the Dianion HP-20 column (highly porous styrene adsorption / desorption resin), elute with 20L of water, 60L of 20% ethanol, and 60L of 40% ethanol in sequence, the flow rate is 20L / 24h, collect the eluted part of 40% ethanol, and wash with water Discretely dissolved, centrifugally filtered, concentrated to 40ml, passed through a Toyopearl HW-40 chromatographic column, eluted with 3L of water and 3L of 10% methanol aqueous solution with a volu...

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Abstract

The invention relates to a method for extracting a 2-phenylacetamide compound from semen lepidii and an application of the 2-phenylacetamide compound and effectively solves the problems about extraction of 2-phenylacetamide compound from semen lepidii and preparation of drugs for treating estrogen deficiency symptoms by use of the 2-phenylacetamide compound. According to the method, stir-fried semen lepidii is extracted three times with water with the weight 10 times that of the semen lepidii, three extracting solutions are mixed and concentrated to an extract under reduced pressure, the extract is subjected to alcohol precipitation with ethanol, precipitates are discarded, a liquid supernatant is centrifuged, filtered, concentrated until no alcohol smell exists and then applied to a Dianion HP-20 column, elution is performed sequentially with water, 20% ethanol and 40% ethanol, the 40% ethanol elution part is collected, dispersion and dissolution are performed with water, centrifugal filtration and concentration are performed, a product passes through a Toyopearl HW-40 chromatographic column, elution is performed sequentially with water and a 10% methanol water solution, the 10% methanol water solution part is collected, dissolved with methanol and left to stand for 24 h, colorless crystals are separated and dried, and 2-phenylacetamide is obtained. The method is novel, unique and easy to operate, 2-phenylacetamide is effectively used for preparing a drug for treating the estrogen deficiency symptoms, and the medical value and the commercial value of semen lepidii are expanded.

Description

technical field [0001] The invention relates to medicine, in particular to a method for extracting 2-phenylacetamide compound from Tinglizi chinensis and its application. Background technique [0002] Phytoestrogens are derived from plants and have similar structures to the mammalian steroid hormone 17β-estradiol. Natural phytoestrogens have lower estrogen-like activity than synthetic estrogens, but they cause fewer side effects such as breast cancer and endometrial cancer, and are safer to use. Therefore, research on phytoestrogens has gradually become a hot spot. [0003] Tinglizi is a commonly used Chinese medicinal material, first recorded in "Shen Nong's Materia Medica", and listed as a low-grade product. The medicine tastes pungent and bitter, and is very cold in nature. It returns to the lung and bladder meridian. The "Tinglizi" included in the 2015 edition of "Chinese Pharmacopoeia" can be divided into north and south. Among them, the dry mature seeds of the crucif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C231/24C07C233/11A61K31/165A61P5/30
CPCC07C231/24A61K31/165A61K36/31C07C233/11
Inventor 郑晓珂曾梦楠李孟李苗张贝贝张志广赵莹莹王小兰冯卫生
Owner HENAN UNIV OF CHINESE MEDICINE
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