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Withanolide compounds and extraction method and application

A technology of lactide compounds and compounds, which is applied in the direction of steroids, digestive system, organic chemistry, etc., can solve the problems that the extraction method and application of withanide compounds have not been reported yet, and achieve the effect of inhibiting release

Active Publication Date: 2017-08-18
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there are no reports on the extraction of withanides, extraction methods and applications from A.

Method used

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  • Withanolide compounds and extraction method and application
  • Withanolide compounds and extraction method and application
  • Withanolide compounds and extraction method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The extraction method of withanide compound, comprises the steps:

[0045] (1) Take the dry stem and leaf (9.5kg) of bitter mulberry (Physalis angulata L.) as raw material, add 9 times the mass fraction of the raw material as a 75% ethanol aqueous solution, reflux extraction twice, each extraction for 2 hours, and combine to obtain Extract, solvent recovery under reduced pressure, obtain total extract (1370g) after concentrating;

[0046] (2) Disperse the total extract into water of 5 times by mass, extract with equal volumes of petroleum ether and ethyl acetate successively, and recover the solvent under reduced pressure from the ethyl acetate extract to obtain 116g of ethyl acetate layer extract;

[0047] (3) The ethyl acetate layer extract is separated by silica gel column chromatography, and the dichloromethane-methanol with volume ratios of 100:1, 50:1, 30:1 and 15:1 are gradient eluted as eluents to obtain Fractions Fr.1, Fr.2, Fr.3 (35g) and Fr.4 (15g);

[0048...

Embodiment 2

[0074] The extraction method of withanide compound, comprises the steps:

[0075] (1) Take the dry stem and leaf of A. fraterna as a raw material, add 8 mass times of the raw material in a volume fraction of 80% ethanol aqueous solution, reflux extraction 3 times, extract for 2 hours each time, combine to obtain the extract, recover the solvent under reduced pressure, and concentrate Obtain the total extract;

[0076] (2) Disperse the total extract into water of 5 times by mass, extract with equal volumes of petroleum ether and ethyl acetate successively, and recover the solvent under reduced pressure from the ethyl acetate extract to obtain the ethyl acetate layer extract;

[0077] (3)-(12) is the same as embodiment 1 (3)-(12).

Embodiment 3

[0079] The extraction method of withanide compound, comprises the steps:

[0080] (1) Take the dry stem and leaf of A. fraterna as a raw material, add 10 times the mass fraction of the raw material as a 60% ethanol aqueous solution, reflux extraction for 3 times, extract for 2 hours each time, combine to obtain the extract, recover the solvent under reduced pressure, and concentrate Obtain the total extract;

[0081] (2) Dispersing the total extract into water of 10 times by mass, extracting with equal volume petroleum ether and ethyl acetate successively, and recovering the solvent from the ethyl acetate extract under reduced pressure to obtain the ethyl acetate layer extract;

[0082] (3)-(12) is the same as embodiment 1 (3)-(12).

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Abstract

The invention discloses withanolide compounds and an extraction method and application. The withanolide compounds have the structures shown in the formula (I). The withanolide compounds can effectively inhibit the release of nitric oxide (NO), and it is indicated that the withanolide compounds can be used as anti-inflammatory drugs.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine extraction, and relates to a withanide compound, an extraction method and an application. Background technique [0002] Physalis angulata L., also known as lantern grass, Tianpaozi, Tianpao grass, Pupu grass, Daebao, etc., is an annual herbaceous plant of the genus Physalis in the family Solanaceae. "Lu Chuan Materia Medica" and "Chinese Materia Medica" have detailed records on the medicinal effects of bitter fennel. Bitter bean has the functions of promoting qi, reducing swelling, diuresis, curing abdominal distension, clearing away heat and detoxification, diuretic and hemostasis, reducing swelling and dispelling stagnation, and can be used to treat throat Swelling and pain, lung abscess, mumps; dysuria, hematuria, gum swelling and pain, pemphigus and other diseases. Folks are mostly used to treat herpes, swollen gums, damp-heat jaundice, sore throat, lung-heat cough and other diseas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00C07J71/00A61P29/00A61P9/12A61P9/10A61P9/04A61P1/16A61P19/02A61P1/00A61P3/10A61P1/18A61P37/06A61P17/06A61P35/02A61P25/16A61P25/28A61P25/24A61P11/00A61P11/06A61P25/00A61P17/00A61P35/00
CPCA61K36/81C07J17/00C07J71/001
Inventor 邱峰孙成鹏陈丽霞康宁
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE