Method for improving yield of cordycepin and thermoduric proteinase of cordyceps militaris (L.) link through liquid-state fermentation method

A technology of Cordyceps militaris and cordycepin, which is applied in the field of microorganisms and can solve the problems of long fermentation time, restrictions on large-scale production of cordycepin, and high cost of culture medium

Active Publication Date: 2017-08-18
XUZHOU HONGYU AGRI TECH
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] At present, it has been reported that Cordyceps militaris is submerged in fermentation, but the content of cordycepin is low, and the fermentation time is long and the cost of medium is high, which limits the large-scale production of cordycepin.
In addition, there is no report about the Cordyceps militaris strain and liquid fermentation preparation method of high-yield heat-resistant protease and high-yield cordycepin

Method used

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Embodiment Construction

[0067] The present invention will be further described below by embodiment:

[0068] 1. Isolation and screening of bacterial strains

[0069] In August 2015, wild Cordyceps militaris was collected in Yimeng Mountain, Yinan County, Shandong Province. The collection site was in a forest with loose soil and rich humus. The ambient temperature was 20-28°C and the air humidity was 70-80%. A total of 9 wild Cordyceps strains were collected, and the Cordyceps was slowly peeled off from the layer, and the surface of the Cordyceps was rinsed repeatedly with clean water until it was cleaned. Blot the surface moisture of Cordyceps dry with sterile filter paper. Then disinfect the surface with 75% ethanol or 0.1 L mercury, and rinse with sterile water again. Then, carefully cut out a small piece from the Cordyceps militaris subunit, put it on the activated solid medium of Cordyceps, and culture it statically at 22-26°C. After the hyphae grow out, observe every day. Select the hyphae w...

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Abstract

The invention discloses a method for improving the yield of cordycepin and thermoduric proteinase of cordyceps militaris (L.) link through a liquid-state fermentation method, and relates to a strain which is preserved in China Committee for Culture Collection Center for general microbiology. By utilizing the strain, through preparation of cordyceps militaris (L.) link strain by liquid fermentation and production of cordycepin by liquid strain fermentation, an experiment result proves that the content of strain deep liquid-state ventilation fermentation cordycepin reaches 4.12g/L, and the activity of the thermoduric proteinase reaches 92554U/ml. Meanwhile, the method disclosed by the invention has the advantages of simple operation, low cost, short period, and easy realization; the raw materials are rich in sources and easy to get, are low in cost and have no pollution to the environment; the prices of the used equipment and reagents are cheap; and large-scale production is convenient.

Description

technical field [0001] The invention relates to a cordyceps militaris strain with high yield of heat-resistant protease and cordycepin, in particular to a method for improving the production of cordycepin and heat-resistant protease by liquid-state fermentation of Cordyceps militaris, belonging to the technical field of microorganisms. Background technique [0002] Cordyceps militaris [Cordyceps militaris (L.) Link], also known as Cordyceps militaris, modern scientific research shows that Cordyceps militaris is rich in active substances, such as cordyceps polysaccharide, cordycepin, cordycepic acid, amino acid, protease, etc. Oxidation and aging, anti-inflammatory and antibacterial, anti-fatigue, inhibition of virus growth, enhancement of immunity and other pharmacological effects and nourishing functions, it is a great medicinal treasure with development potential. Among the active ingredients of Cordyceps militaris, cordycepin is the main active ingredient (especially nucl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/58C12P19/40C12R1/645
CPCC12N1/14C12N9/58C12P19/40C12N1/145C12R2001/645
Inventor 高兆建杨杰孙会刚曹泽虹曹建冬沈彬彬顾强康志锋
Owner XUZHOU HONGYU AGRI TECH
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