Kit for rapidly extracting RNA of influenza viruses

A kit and rapid technology, applied in the field of molecular biology detection, can solve the problems of long purification cycle, complex extraction steps, and unfavorable customers to quickly extract and purify RNA.

Active Publication Date: 2017-08-18
GUANGZHOU YIXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent Nos. 201010281633.6 and 201510023368.4 disclose methods for extracting viral DNA / RNA, but these reagents are relatively complex in composition, the extraction steps are relatively complicated, and the purification cycle is long, which is not conducive to the rapid extraction and purification of RNA by customers

Method used

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  • Kit for rapidly extracting RNA of influenza viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The final concentration of the virus lysis binding solution is composed of: 20mM sucrose, 10mM Tris-HCl hydrochloride (Tris-HCl), 14% (V / V) polyether alcohol, the solvent is high-pressure sterilized water, and the virus lysis combination The pH value of the solution is 7.0. The method of using the virus lysis binding solution for nucleic acid detection: take a 1.5ml centrifuge tube without RNase, add 200 μl of the virus lysis binding solution to the tube, and then add 200 μl of fresh influenza samples, shake and mix Let stand at room temperature for 5 minutes.

[0022] The sample volume extracted by QIAamp Viral RNA Mini Kit was 200 µl, and the elution volume was 100 µl.

[0023] After the extraction is completed, take 5 µl of the above nucleic acid RNA extraction solution as a template, and use Daan Gene’s Influenza A Virus Nucleic Acid Detection Kit (one-step PCR fluorescence method) for detection. The detection results are shown in Table 1 below and attached figure ...

Embodiment 2

[0028] The experimental method is the same as in Example 1, the only difference is that the final concentration of the virus lysis binding solution is composed of: 30mM sucrose, 30mM Tris-HCl, 12% (V / V) polyether alcohol, the solvent is autoclaved water, and the virus lysis binding solution The pH value is 6.5.

Embodiment 3

[0030] The experimental method is the same as in Example 1, the only difference is that the final concentration of the virus lysis binding solution is composed of: 50mM sucrose, 50mM Tris-HCl, 10% (V / V) polyether alcohol, the solvent is autoclaved water, and the virus lysis binding solution The pH value is 7.5.

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Abstract

The invention belongs to the field of biological detection and discloses a kit for rapidly extracting RNA of influenza viruses. The kit comprises virus splitting and binding liquid which contains polyether alcohol, sucrose and Tris-HCl. The kit disclosed by the invention has the advantages that by utilization of the kit, the nucleic acid of the influenza viruses can be released only by one-step operation, and the following substances can be used for fluorescence PCR (Polymerase Chain Reaction) detection.

Description

technical field [0001] The invention relates to the technical field of molecular biology detection, more specifically, to a kit for rapidly extracting viral RNA. Background technique [0002] Nucleic acid extraction is the first step in the entire technical process of nucleic acid detection, and it is also one of the most critical methods in molecular biology. It is the basis of downstream nucleic acid detection and scientific research. The quality and integrity of the extracted nucleic acid will directly affect the results of clinical research or diagnosis. The general nucleic acid extraction process includes two steps: sample lysis and purification. Cleavage refers to the release of nucleic acid in the sample into the reaction system, while purification refers to the complete separation of nucleic acid from other components in the reaction system, such as proteins, salts and other impurities. Commonly used RNA extraction methods include TRIZOL method, guanidine isothiocy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12Q1/68
CPCC12N15/1003C12Q1/6806C12Q2527/125
Inventor 吴英松刘天才杨学习陈瑶
Owner GUANGZHOU YIXIN BIOTECH CO LTD
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