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Application of alkane oxygen carrier in increasing yield of polymalic acid of aureobasidium pullulans

A technology of Aureobasidium pullulans and polymalic acid, applied in the field of microorganisms, can solve the problems of poor concentration of pullulan and achieve the effect of inhibiting synthesis and increasing yield

Active Publication Date: 2017-08-18
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention discloses the application of alkane oxygen carrier in improving the yield of polymalic acid of Aureobasidium pullulans to solve the problem in the prior art that the effect of reducing the concentration of pullulan in the product is not good

Method used

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  • Application of alkane oxygen carrier in increasing yield of polymalic acid of aureobasidium pullulans

Examples

Experimental program
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Effect test

Embodiment 1

[0031] A. pullulans HA-4D with the preservation number CGMCC No. 7.208 was inoculated into a 500 mL shaker flask containing 100 mL of seed medium, and cultured on a shaking table at a temperature of 25°C and a rotation speed of 200 rpm for 48 hours. Then take 10 mL of the seed solution and inoculate it in a 500 mL shake flask, which contains 90 mL of basic fermentation medium, and additionally add 1 mL of n-heptane, and then culture in a shaker at a temperature of 25 °C and a speed of 200 rpm for 144 hours. The results showed that the yield of polymalic acid in this example was 33.8g / L, and the yield of pullulan was 2.5g / L.

[0032] At the same time, a comparative experiment was carried out according to the same method as above, except that the basic fermentation medium was used and n-heptane was not added. The results showed that the yield of polymalic acid in this comparative example was 30.3 g / L, and the yield of pullulan was 5.9 g / L.

[0033] Compared with Comparative Exa...

Embodiment 2

[0035] Aureobasidium pullulans HA-4D with the preservation number CGMCC No.7.208 was inoculated in a 500mL shake flask containing 100mL seed medium, and cultured on a shaking table at a temperature of 25°C and a rotation speed of 200rpm for 48h, and the seed solution was prepared 3 bottles. Then take 300mL of seed solution and inoculate it in a 5L mechanically stirred fermenter. The fermenter is equipped with 2.7L of basic fermentation medium, and an additional 30mL of n-heptane is added. Fermentation was carried out under the conditions, and when the concentration of residual sugar in the fermentation broth was lower than 10g / L, 300g / L glucose was continuously added to the tank at a flow rate of 12mL / h, and the fermentation was terminated after 168 hours. In this example, the yield of polymalic acid was 102.8g / L, and the yield of pullulan was 8.7g / L.

[0036]At the same time, a comparative experiment was carried out according to the same method as above, except that the basi...

Embodiment 3

[0039] A. pullulans HA-4D with the preservation number CGMCC No. 7.208 was inoculated into a 500 mL shaker flask containing 100 mL of seed medium, and cultured on a shaking table at a temperature of 25°C and a rotation speed of 200 rpm for 48 hours. Then take 10 mL of the seed solution and inoculate it in a 500 mL shake flask, which contains 90 mL of basic fermentation medium, and additionally add 1 mL of n-dodecane, and then culture in a shaker at a temperature of 25°C and a speed of 200 rpm for 144 hours. The results showed that the yield of polymalic acid in this example was 32.9g / L, and the yield of pullulan was 2.7g / L.

[0040] At the same time, a comparative experiment was carried out according to the same method as above, except that the basic fermentation medium was used and n-dodecane was not added. The results showed that the yield of polymalic acid in this comparative example was 30.3 g / L, and the yield of pullulan was 5.9 g / L.

[0041] Compared with Comparative Ex...

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Abstract

The invention discloses application of an alkane oxygen carrier in increasing yield of polymalic acid of aureobasidium pullulans. The application method comprises the following steps: activating aureobasidium pullulans and then fermenting in a culture medium containing the alkane oxygen carrier. Compared with the prior art, the invention discloses a method for increasing the yield of polymalic acid and restraining pullulan synthesis by utilizing the alkane oxygen carrier. A low-concentration alkane oxygen carrier is used for establishing a directional regulation strategy, the carbon metabolism flow direction regulation for the polymalic acid and pullulan synthesis is realized, the synthesis of the side product pullulan is restrained, the yield of the main product polymalic acid is increased, and meanwhile, the added alkane oxygen carrier within the concentration scope will not restrain the growth of the aureobasidium pullulans cells.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to the application of alkane oxygen carriers in increasing the polymalic acid yield of Aureobasidium pullulans. Background technique [0002] Polymalic acid (polymalic acid) is a kind of polymer with malic acid as the only monomer, which is connected with each other through ester bonds. It was first discovered by Shimada et al. In 1979, Vert et al prepared the polymer by chemical synthesis for the first time. Polymalic acid has good water solubility, and its hydrolyzed monomeric malic acid can be absorbed by participating in the tricarboxylic acid cycle in the living body without any toxic and side effects on the human body. The good water solubility, biodegradability and biocompatibility of polymalic acid make polymalic acid and its derivatives have quite broad application prospects in many fields such as biomedicine, biomaterials and food engineering. Polymalic acid prepared ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62C12N1/38C12R1/645
CPCC12N1/38C12P7/625
Inventor 夏军徐继明刘晓燕许家兴
Owner HUAIYIN TEACHERS COLLEGE
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