Molecular marker caps1516 tightly linked to soybean blight resistance gene and its application

Abstract

The invention provides a molecular marker CAPs1516 closely linked to a soybean blight resisting gene RpsHC18 and applications thereof. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1; the fragment size of marker is 541bp; the marker is located in the RpsHC18 candidate gene of soybean chromosome 3; the genetic distance from the RpsHC18 is 0cm; and currently known other 20 soybean blight resisting genes can be effectively distinguished. The molecular marker CAPs1516 can be used for the blight resisting gene identification of soybean germplasm resources and blight resisting molecular assisted breeding so as to accelerate the breeding progress.

Description

technical field [0001] The invention belongs to the fields of agricultural biotechnology engineering and crop disease-resistant genetic breeding, and specifically relates to a molecular marker co-separated with soybean disease-resistant gene RpsHC18 and its application. Background technique [0002] Soybean blight caused by Phytophthora sojae Kaufmann & Gerdemann is a devastating disease in soybean production. The disease has occurred in my country's Heilongjiang Province, Anhui, Fujian, Jilin, Xinjiang and other provinces. Phytophthora sojae can infect soybeans in all growth stages of soybeans, causing root rot, stem rot, plant dwarfing, withering and death. Generally, the yield of fields is reduced by 10%-30%, and in severe fields it can reach 60%-90%. even lead to miscarriage. At present, the annual economic loss caused by soybean blight is about 1 billion US dollars worldwide (Tyler, 2007). [0003] The most economical, effective, and environmentally safe way to contr...

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Problems solved by technology

The population structure of Phytophthora sojae is complex, and it is easy to produce new virulence types

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