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Molecular marker co-segregated with soybean blight resistance gene rpszs18 and its application

A molecular marker and anti-epidemic technology, which is applied in the field of agricultural biotechnology engineering and crop disease-resistant genetic breeding, can solve problems such as the complex structure of Phytophthora soybean population, achieve the effects of improving breeding efficiency, reducing yield loss, and overcoming the long cycle

Inactive Publication Date: 2019-04-16
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The population structure of Phytophthora sojae is complex, and it is easy to produce new virulence types

Method used

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  • Molecular marker co-segregated with soybean blight resistance gene rpszs18 and its application
  • Molecular marker co-segregated with soybean blight resistance gene rpszs18 and its application
  • Molecular marker co-segregated with soybean blight resistance gene rpszs18 and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Example 1 The acquisition of the candidate gene of soybean blight resistance gene RpsZS18 and its co-segregated molecular marker ZCindel246000

[0043] 1. Genetic analysis of resistance in precocious 18

[0044] F 1 Generation, F 1 F 2 Generation, F 2 75 F 3 Genetic analysis of resistance in pedigrees, identification of disease resistance genes and mapping of populations. Using the hypocotyl wound inoculation method, 20-25 plants identified in each family were analyzed for their resistance to Phytophthora sojae strain PsUSAR2. At 24°C after inoculation, under the condition of 100% humidity, keep moist for 48 hours, then transfer to the greenhouse for cultivation, and carry out disease investigation after 4 days ( figure 1 ). Referring to the evaluation criteria of Gordon et al. (2006), it is stipulated that families with a plant mortality rate of less than 20% are pure and resistant families, those with a mortality rate greater than 80% are pure and susceptible f...

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Abstract

The invention provides a molecular marker ZCindel246000 coseparated from a soybean epidemic disease resisting gene RpsZS18, and an application thereof. The marker is positioned between the SSR markers ZCSSR33 and ZCSSR46 of a soybean No.2 chromosome, and has a genetic distance of 0cM to the gene RpsZS18. A new SSR marker is exploited by using a soybean SSR marker in an interval and using a soybean genome sequence according to a preliminary positioning result with an F3 column generated through hybridizing and selfing disease resisting variety Zaoshu 18 used as a male parent and a susceptible variety Williams used as a female parent as a mapping colony, and a disease resisting gene contained in the Zaoshu 18 is finely positioned. The disease resisting gene RpsZS18 is positioned between the ZCSSR33 (0.7cM) and ZCSSR46 (2.8cM) of the No.2 chromosome, and is coseparated from an SSR marker Satt172. An RpsZS18 candidate gene is predicated according to gene note in the new positioning interval, the Indel marker ZCindel24600 is exploited according to the candidate gene site sequence difference predicated by resistant and susceptible parents, a colony verification result shows that the marker is a coseparated marker, and the Indel marker can be used in auxiliary breeding of soybean epidemic disease resistance.

Description

technical field [0001] The invention belongs to the fields of agricultural biotechnology engineering and crop disease-resistant genetic breeding, and specifically relates to a molecular marker co-separated with soybean disease-resistant gene RpsZS18 and its application. Background technique [0002] Soybean blight caused by Phytophthora sojae Kaufmann & Gerdemann is a devastating disease in soybean production. The disease has occurred in my country's Heilongjiang Province, Anhui, Fujian, Jilin, Xinjiang and other provinces. Phytophthora sojae can infect soybeans in all growth stages of soybeans, causing root rot, stem rot, plant dwarfing, withering and death. Generally, the yield of fields is reduced by 10-30%, and in severe fields, it can reach 60-90%. extinct. At present, the annual economic loss caused by soybean Phytophthora worldwide is about 1 billion US dollars (Tyler, 2007). [0003] The most economical, effective, and environmentally safe way to control soybean b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6895
Inventor 朱振东钟超孙素丽李银萍段灿星
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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