Molecular marker co-segregated with soybean blight resistance gene rpszs18 and its application
A molecular marker and anti-epidemic technology, which is applied in the field of agricultural biotechnology engineering and crop disease-resistant genetic breeding, can solve problems such as the complex structure of Phytophthora soybean population, achieve the effects of improving breeding efficiency, reducing yield loss, and overcoming the long cycle
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[0042] Example 1 The acquisition of the candidate gene of soybean blight resistance gene RpsZS18 and its co-segregated molecular marker ZCindel246000
[0043] 1. Genetic analysis of resistance in precocious 18
[0044] F 1 Generation, F 1 F 2 Generation, F 2 75 F 3 Genetic analysis of resistance in pedigrees, identification of disease resistance genes and mapping of populations. Using the hypocotyl wound inoculation method, 20-25 plants identified in each family were analyzed for their resistance to Phytophthora sojae strain PsUSAR2. At 24°C after inoculation, under the condition of 100% humidity, keep moist for 48 hours, then transfer to the greenhouse for cultivation, and carry out disease investigation after 4 days ( figure 1 ). Referring to the evaluation criteria of Gordon et al. (2006), it is stipulated that families with a plant mortality rate of less than 20% are pure and resistant families, those with a mortality rate greater than 80% are pure and susceptible f...
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