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A technology of stem tips and grafted seedlings, which is applied in the field of planting, can solve the problems of low survival rate of grafting, excessive wounds, easy loss of water, etc., and achieve the effect of improving survival rate of grafting, increasing contact space, and improving survival rate
Active Publication Date: 2017-08-29
SOUTHWEST FORESTRY UNIVERSITY
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Problems solved by technology
Micro-bud grafting with T” grafting method has a large wound, and is exposed to the air, so it is easy to lose water. In addition, there are disadvantages that the rootstock and scion are not stable, and the incision is easy to dry; the wound of split grafting is too large, and the scion is easy to dehydrate and die. Low survival rate; △ connection method: 45° to the stem segment, cut off on both sides, and then cross-cut once
Therefore, the procedure of the operation method is relatively complicated, the rootstock is easily damaged, and the grafting is difficult and time-consuming.
[0005] Therefore, is it possible to provide a method for grafting shoot tip microbuds to solve the problem of easy inactivation of grafted scions and unstable combination of stock and ear in conventional grafting techniques, so as to improve the survival rate of grafting, thereby solving the technical problems urgently to be solved by those skilled in the art?
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Embodiment 1
[0030] Embodiment 1 grapefruit in vitro puncture method stem tip micro-bud grafting method
[0031] 1. Experimental materials
[0032] The seeds of Mansailong pomelo from the experimental forest farm in Puwen Town, Xishuangbanna Prefecture, Yunnan Province were used as rootstocks, and the scions were the buds induced by in vitro culture of grapefruit. The stem tip of the adventitious bud is used as the scion, and the rootstock is the rootstock seed. After in vitro culture, the seedling that has just germinated for 30 days is taken; 1mg / L 6-BA, grafting knife, high-power stereo microscope,
[0033] 2. Experimental method
[0034] Take 120 grapefruit stems for in vitro culture, after the grapefruit stems induce adventitious buds, take the stem tip as a scion, take 120 rootstock seeds for in vitro culture, take the seedlings that have just germinated for 30 days, and cut off the rootstock Leave the stem length of 1.5-2cm at the top as the rootstock, then use the "T" connection ...
Embodiment 2
[0048] Embodiment 2 grapefruit field puncture method stem tip micro-bud grafting method
[0049] In the field experiment, 120 rootstocks were sown in advance, and 30 days later, when the rootstocks germinated to a certain extent, 120 grapefruit adult annual stem tips were taken as scions, and the above-mentioned "T" grafting method, split grafting method, △ grafting method, and puncture method were used respectively. Four methods are used for shoot tip microbud grafting.
[0050] 1. Experimental materials
[0051] The seeds of Mansailong pomelo were taken from the experimental forest farm of Puwen Town, Xishuangbanna Prefecture, Yunnan Province. The scion was grapefruit buds, and the rootstock was 30-day-old seedlings, 1 mg / L 6-BA, and a grafting knife.
[0052] 2. Experimental method
[0053] Use "T" connection method, cleave connection method, △ connection method, and puncture method to graft shoot tip microbuds respectively, wherein:
[0054] Experimental steps of field ...
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Abstract
The invention belongs to the field of fruit tree planting and particularly relates to a micro-bud grafting puncture method and an application thereof, in particular to a stem-tip micro-bud grafting puncture method. The stem-tip micro-bud grafting puncture method includes: taking a scion 1-2mm long and containing 3-4 leaf primordias and soaking the scion in 1mg / L 6-BA for 10 minutes for pretreatment; under aseptic conditions, topping off a stock leaving a stem 1.5-2cm in length, longitudinally cutting through the position 5-8mm from the top, allowing the cut to be through with the joint of an xylem one third of the stem section and a phloem so that I-shaped cuts are formed in two sides of the stem section, and shifting the 3-4 leaf primordias retained on the scion to the cuts. With the method, problems that traditional stem-tip micro-bud grafting methods of an inverted 'T' grafting method, a splitting grafting method, a triangle grafting method and the like are difficult in operation, large in cut, unstable in scion, prone to water loss in growth and difficult to combine with the stock fully are solved, survival rate of stem-tip micro-bud grafting is increased, production efficiency of virus-free seedlings is increased, and production efficiency for further non-toxic seedling breeding is improved.
Description
technical field [0001] The invention belongs to the field of planting, and relates to a method for grafting stem tip microbuds, in particular to a grafting and puncturing method for stem tip microbuds. Background technique [0002] With the development of modern science and technology, asexual reproduction is often used in the cultivation process of fruit trees. Although asexual reproduction can increase production efficiency, fruit trees will be cross-infected with various viral diseases and similar viral diseases during production. Many infectious diseases such as citrus huanglongbing Not only is it a devastating disease, but there is currently no drug that can inhibit the HLB virus without harming the plant itself. Therefore, it is imminent to establish a complete grapefruit virus-free seedling propagation system. At present, there are four methods for controlling virus-like viroids in fruit trees, among which shoot tip microbud grafting has been proved to be able to obt...
Claims
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Application Information
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