A method for the detection of nicotine optical isomers in mainstream cigarette smoke by normal phase liquid chromatography-tandem mass spectrometry
A technology of cigarette mainstream smoke and normal phase liquid chromatography, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low sensitivity, long analysis time, and difficulty in achieving accurate quantification, and achieve high sensitivity and short analysis time , the effect of improving the sensitivity
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Embodiment 1
[0024] (1) Smoke the balanced and screened cigarettes according to the standard conditions specified in YC / T29-1996, and use Cambridge filter to capture the particulate matter in the mainstream smoke of 5 cigarettes. Fold the Cambridge filter disc for trapping particulate matter into a 50 mL Erlenmeyer flask with a stopper, add 3 mL of 5.0% NaOH solution, let it stand for 15 min, and accurately add d 3 - 20 mL of nicotine racemate in n-hexane, racemic d 3 -The concentration of nicotine was 5 mg / mL, ultrasonically extracted for 30 min, and the supernatant was taken to pass through the organic phase filter membrane added with 2.0 g of anhydrous sodium sulfate in the syringe, and the filtrate was analyzed by normal phase liquid chromatography-tandem mass spectrometry.
[0025] (2) Instrumental analysis: analysis by normal phase liquid chromatography-tandem mass spectrometry, the chromatographic column used is Daicel chiral chromatographic column CHIRALPAK ® IC, the size is 4.6 ...
Embodiment 2
[0027] Five typical cigarette samples were selected, and the content of nicotine optical isomers in the total particulate matter of mainstream cigarette smoke was determined according to the analysis method described in Example 1. The results are shown in Table 3.
[0028] Table 3: Contents of nicotine optical isomers in mainstream cigarette smoke (mg / cigarette)
[0029] Sample serial number S-(-)-nicotine R-(+)-nicotine 1# 0.1 0.003 2# 0.3 0.008 3# 0.6 0.019 4# 0.8 0.022 5# 1.1 0.033
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