A method for the detection of nicotine optical isomers in mainstream cigarette smoke by normal phase liquid chromatography-tandem mass spectrometry

A technology of cigarette mainstream smoke and normal phase liquid chromatography, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low sensitivity, long analysis time, and difficulty in achieving accurate quantification, and achieve high sensitivity and short analysis time , the effect of improving the sensitivity
CN107132289BActive Publication Date: 2019-09-24ZHENGZHOU TOBACCO RES INST OF CNTC
2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHENGZHOU TOBACCO RES INST OF CNTC
Publication Date
2019-09-24

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

A normal phase liquid chromatography-tandem mass spectrometry detection method of nicotine optical isomers in the mainstream smoke of cigarettes is characterized in that total particulate matters in smoke are captured by a Cambridge filter, nicotine is extracted with NaOH solution and n-hexane, the separation of the nicotine optical isomers is realized by adopting chiral chromatography-normal phase chromatography, isopropanol is introduced by a post-column high pressure infusion pump and is shunted, and tandem mass spectrometry is combined to carry out analysis. The method has the following advantages: 1, the nicotine optical isomers in the mainstream smoke of cigarettes are analyzed through adopting the normal phase liquid chromatography-tandem mass spectrometry; 2, the isopropanol is introduced by the isopropanol and is shunted to compensate the volume of a polar solvent entering a mass spectrometer, reduce the volume of a non-polar solvent and improve the ionization efficiency, so requirements of the liquid chromatography-tandem mass spectrometry are met; and 3, the chromatographic resolution of S-(-)-nicotine and R-(+)-nicotine is higher than that of existing methods (the retention time difference is 1 min), and the analysis time is short (only 17 min).
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical research on the separation, analysis and detection of chiral isomers in complex samples, and specifically relates to a normal phase liquid chromatography-tandem mass spectrometry detection method for nicotine optical isomers in mainstream cigarette smoke, mainly a Cambridge filter Collect the total particle phase of cigarette mainstream smoke, extract nicotine on the filter with NaOH solution-n-hexane, use chiral chromatography column to separate nicotine optical isomers, introduce isopropanol into the high-pressure infusion pump behind the column and split the flow, combined with tandem mass spectrometry. Background technique

[0002] Nicotine is the most abundant alkaloid in tobacco and smoke, and is the main driving force for human smoking. Nicotine has two optical isomers, namely S-(-)-nicotine and R-(+)-nicotine. The two have completely different metabolic mechanisms and physiological characteristics, and t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More