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Standard sample of coliform bacteria in milk powder and its preparation method

A standard sample, coliform technology, applied in biochemical equipment and methods, microorganism-based methods, bacteria, etc., to solve problems such as foodborne diseases

Active Publication Date: 2020-07-07
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the number of coliform bacteria exceeds a certain limit, it increases the risk of pathogenic E. coli and eventually leads to foodborne illness

Method used

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  • Standard sample of coliform bacteria in milk powder and its preparation method
  • Standard sample of coliform bacteria in milk powder and its preparation method
  • Standard sample of coliform bacteria in milk powder and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Coliform standard sample in milk powder, including target flora and background flora, said target flora consists of Escherichia coli (E.coil), Klebsiella pneumoniae (Klebsiella pneumoniae), Enterobacter cloacae (Enterobactercloacae) ), Enterobacter aerogenes (Enterobacter aerogenes), and the background flora is composed of Staphylococcus aureus, Bacillus cereus, and Rhodococcusequi.

[0028] The sample is based on trehalose, skimmed milk powder and sterile water, wherein the volume fraction of trehalose is 10%, and the volume fraction of skimmed milk powder is 15%.

[0029] The target concentration of the target flora in the sample is 10 2 ~10 3 CFU / mL, the target concentration of background flora is 10 3 CFU / mL.

Embodiment 2

[0031] Such as figure 1 As shown, a method for preparing a coliform standard sample in milk powder in Example 1 includes four steps of sample addition bacterial strain selection, preparation of lyoprotectant, sample freeze-drying, sample uniformity and stability test ,Specific steps are as follows:

[0032] 1. Selection of sample addition strains

[0033] According to the dominant flora: Escherichia coli (E.coil), Klebsiella pneumoniae (Klebsiellapnenmoniae), Enterobacter cloacae (Enterobacter cloacae), Enterobacter aerogenes (Enterobacter aerogenes), background flora: Staphylococcus aureus (Staphylococcus aureus), Bacillus cereus (Bacillus cereus), and Rhodococcus equi (Rhodococcus equi) were selected as standard strains. All standard strains were purchased from institutions designated by the government, and a strain certificate was attached to ensure the traceability of the strains.

[0034] 2. Preparation of lyoprotectant

[0035] The sample is prepared with trehalose, s...

Embodiment 3

[0072] Each step of the preparation method of the coliform standard sample in milk powder described in this embodiment is all the same as in Example 2, and the different technical parameters are: in the preparation of the bacterial suspension, each target bacteria in the target bacterial suspension The concentration is according to 5×10 3 CFU / mL preparation, the concentration of each background bacteria in the background flora suspension according to 10 3 CFU / mL preparation; freeze-drying process 45h.

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Abstract

The invention belongs to the field of quality control over the aspect of microbiological detection, and particularly relates to a milk powder coliform standard sample and a preparation method thereof. The milk powder coliform standard sample comprises target flora and background flora. The target flora is prepared from escherichia coli, klebsiella pneumoniae, enterobacter cloacae and enterobacter aerogenes, and the background flora is prepared from rhodococcus equi, bacillus cereus and staphylococcus aureus. The uniformity and stability of the sample meet requirements for standard samples, and the stability of the standard sample can be ensured to the maximum extent, that is, even though the content of the escherichia coli changes during transportation and storage, the total amount of coliform is not affected statistically; and the preparation method of the sample is simple in process and high in success rate.

Description

technical field [0001] The invention belongs to the quality control field of microbiological detection, and in particular relates to a standard sample of coliform bacteria in milk powder and a preparation method thereof. Background technique [0002] The field of microbiological testing of milk powder is very special. At present, there is no precedent for the preparation of milk powder microbiological standard samples and large-scale production by institutions at home and abroad, which is still a blank field. At present, although some institutions prepare microbiological standard samples in food, their uniformity and stability can hardly meet the requirements, and transportation is extremely difficult; Laboratory quality control and other needs. [0003] Coliform group is not a bacteriological classification name, it does not represent a certain or a certain genus of bacteria, but refers to a group of bacteria related to fecal contamination with certain characteristics, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/10C12N1/20C12N1/04C12R1/19C12R1/22C12R1/01C12R1/445C12R1/085
CPCC12N1/04C12N1/20C12Q1/10
Inventor 卢行安吴孝槐顾文佳赵红阳王秀君王伟孙英健陈若晞
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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