Candida albicans applicability testing strain and preparation method thereof

A Candida albicans, applicability technology, applied in the field of Candida albicans applicability test strains and its preparation, can solve the problems of threatening the life of patients and infection caused by patients

Active Publication Date: 2019-05-10
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If Candida albicans exists in the medicine, it is very easy to cause infection to the patient, directly threatening the life of the patient

Method used

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  • Candida albicans applicability testing strain and preparation method thereof
  • Candida albicans applicability testing strain and preparation method thereof
  • Candida albicans applicability testing strain and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Candida albicans applicability test strain contains only one target bacterium: Candida albicans, the target concentration of the target bacterium is 500-2000 CFU / cartridge, and each bottle contains 0.2g of freeze-dried powder. The applicability test strain is based on trehalose, gelatin and sterile water, wherein the volume fraction of trehalose is 10%, and the volume fraction of gelatin is 1%.

[0030] The sample belongs to the bacterial strain used in the applicability test in drug inspection, and is used in the applicability test in the process of drug microbiological testing. The specific test process steps are as follows:

[0031] After the sample is opened, immediately add 2 mL of sterile water for rehydration, vortex and shake to mix well, take 100 μL to inoculate into the liquid medium to be tested or spread on the medium to be tested, and incubate at 30-35°C for 3-5 sky. Observe whether the growth is good, and whether the colony size and morphological charact...

Embodiment 2

[0033] Such as figure 1 Shown, the preparation method of Candida albicans applicability test strain in a kind of embodiment 1, comprises the preparation of sample adding bacterial strain, the preparation of lyoprotectant, sample lyophilization, sample homogeneity and stability test, sample fixed value Five steps, the specific steps are as follows:

[0034] 1. Selection of sample addition strains

[0035] Contains only one target bacterium: Candida albicans, and the strains are all purchased from institutions designated by the government with strain certificates to ensure the traceability of the strains.

[0036] 2. Preparation of lyoprotectant

[0037] The sample was prepared with trehalose, gelatin and sterile water as the matrix (volume fraction: trehalose 10%, gelatin 1%) to prepare a lyoprotectant.

[0038] 3. Sample freeze-drying

[0039] (1) Strain recovery and passage

[0040] Inoculate the standard strains into nutrient agar slant medium, revive and grow them at 3...

Embodiment 3

[0081] The steps of the preparation method of the Candida albicans applicability test strain described in this example are the same as in Example 2, and the different technical parameters are: freeze-drying process 45h.

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Abstract

The invention belongs to the field of quality control in microbiological detection of medicine and particularly relates to a candida albicans applicability testing strain and a preparation method thereof. The candida albicans applicability testing strain only contains a certain amount of candida albicans. The uniformity and the stability of the strain meet the requirements for the applicability testing strain, the number of bacteria contained in the strain is within the applicability testing standard requirement range, the requirements for applicability tests, growth promotion tests and the like in the microbial detection process of medicines are met to the greatest extent, and a sample preparation method is simple in process and high in success rate.

Description

technical field [0001] The invention belongs to the quality control field of microbiological detection, and in particular relates to a Candida albicans applicability test strain and a preparation method thereof. Background technique [0002] Due to the particularity of drugs, the field of drug microbiology inspection is very special. It is required that the culture medium and other test materials need to be checked for applicability before each test, and the concentration range requirements for the test strains are specified. Most of the existing pure strains have not been quantified and can only be used qualitatively, and there are only a few quantitative strains. For such quantitative strains, uniformity and stability are particularly important. The existing quantitative strains have problems such as poor stability, high bacterial content, unable to meet the test requirements, and high requirements for transportation conditions. [0003] Candida albicans is a kind of fung...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/04C12Q1/04C12R1/725
Inventor 卢行安吕涵阳吴孝槐瞿洪仁单耕赵红阳孙英健王静
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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