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Pseudomonas aeruginosa applicability test strain and preparation method thereof

A Pseudomonas aeruginosa, applicability technology, applied in the field of Pseudomonas aeruginosa applicability test strains and preparation thereof, can solve problems such as life-threatening patients, patient infection and the like

Pending Publication Date: 2019-07-05
浙江宝录检测技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If there is Pseudomonas aeruginosa in the medicine, it is very easy to cause infection to the patient, directly threatening the life of the patient

Method used

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  • Pseudomonas aeruginosa applicability test strain and preparation method thereof
  • Pseudomonas aeruginosa applicability test strain and preparation method thereof
  • Pseudomonas aeruginosa applicability test strain and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The Pseudomonas aeruginosa applicability test strain contains only one target bacterium: Pseudomonas aeruginosa (Pseudomonas aeruginosa), the target concentration of the target bacterium is 500-2000 CFU / cartridge, and each cartridge is equipped with 0.2 g of freeze-dried powder. The applicability test strain is based on trehalose, gelatin and sterile water, wherein the volume fraction of trehalose is 10%, and the volume fraction of gelatin is 1%.

[0030] The sample belongs to the bacterial strain used in the applicability test in drug inspection, and is used in the applicability test in the process of drug microbiological testing. The specific test process steps are as follows:

[0031] After the sample is opened, immediately add 2 mL of sterile water for rehydration, vortex and shake to mix well, take 100 μL to inoculate into the liquid medium to be tested or spread on the medium to be tested, and incubate at 30-35°C for 18-24 hours . Observe whether the growth is g...

Embodiment 2

[0033] Such as figure 1 Shown, the preparation method of Pseudomonas aeruginosa applicability test bacterial strain in a kind of embodiment 1, comprises the preparation of sample addition bacterial strain, the preparation of lyoprotectant, sample lyophilization, sample homogeneity and stability test, sample There are five steps to determine the value, the specific steps are as follows:

[0034] 1. Selection of sample addition strains

[0035] Contains only one target bacterium: Pseudomonas aeruginosa, and the strains are all purchased from institutions designated by the government with strain certificates to ensure the traceability of the strains.

[0036] 2. Preparation of lyoprotectant

[0037] The sample was prepared with trehalose, gelatin and sterile water as the matrix (volume fraction: trehalose 10%, gelatin 1%) to prepare a lyoprotectant.

[0038] 3. Sample freeze-drying

[0039] (1) Strain recovery and passage

[0040] Inoculate the standard strains into nutrient...

Embodiment 3

[0080] The steps of the preparation method of the Pseudomonas aeruginosa applicability test strain described in this example are the same as in Example 2, and the different technical parameters are: freeze-drying process 45h.

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Abstract

The invention belongs to the field of quality control in the field of medicine microbiology detection, and particularly relates to a pseudomonas aeruginosa applicability test strain and a preparationmethod thereof. The pseudomonas aeruginosa applicability test strain only contains a certain amount of pseudomonas aeruginosa. The uniformity and the stability meet the requirement of applicability test strains, the number of bacteria in the strain is within the requirement range of applicability test standards, the requirements of applicability tests, growth promotion tests and the like in the microbial detection process of medicines are met to the greatest extent, and the sample preparation method is simple in process and high in success rate.

Description

technical field [0001] The invention belongs to the quality control field of microbiological detection, and in particular relates to a Pseudomonas aeruginosa applicability test strain and a preparation method thereof. Background technique [0002] Due to the particularity of drugs, the field of drug microbiology inspection is very special. It is required that the culture medium and other test materials need to be checked for applicability before each test, and the concentration range requirements for the test strains are specified. Most of the existing pure strains have not been quantified and can only be used qualitatively, and there are only a few quantitative strains. For such quantitative strains, uniformity and stability are particularly important. The existing quantitative strains have problems such as poor stability and high requirements for transportation conditions. [0003] Pseudomonas aeruginosa, formerly known as Pseudomonas aeruginosa, is a common conditional p...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/04C12R1/385
CPCC12N1/20C12Q1/04
Inventor 赵红阳谢艳王倩闫平平杨洁王伟
Owner 浙江宝录检测技术有限公司
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