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Biological logic gate and logic circuit construction method based on nucleic acid

A technology of logic circuits and construction methods, which is applied in the research field of computational biology and can solve problems such as poor practical applicability

Inactive Publication Date: 2017-09-12
QINGDAO UNIV OF SCI & TECH
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  • Description
  • Claims
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Problems solved by technology

[0004] At present, researchers at Imperial College London have successfully constructed a "biological logic gate" that can be used to process information by using intestinal bacteria and DNA fragments. Applicability is not strong

Method used

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  • Biological logic gate and logic circuit construction method based on nucleic acid
  • Biological logic gate and logic circuit construction method based on nucleic acid
  • Biological logic gate and logic circuit construction method based on nucleic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0037] Example: Construction of a YES gate

[0038] 1. Use the kit to extract micro-RNA in tumor cells and use it as a signal input chain.

[0039] Second, the construction and operation of the operation module.

[0040] (1) Establishment of the bottom chain of the computing module

[0041] The operation module bottom chain (S-MBs) is formed by connecting the bottom chain nucleic acid sequence (S) and magnetic beads (MBs), and its preparation method is to mix 20 μL (10 -6 M) DNA was added to carboxyl-modified magnetic beads activated with 200 μL EDC (0.8M) for 30 minutes, then 200 μL NHS (0.2M) was added, and reacted at 37 ° C for 12 hours, then magnetically separated using a magnetic stand, and the upper layer was removed The supernatant containing the nucleic acid sequence of the bottom strand that is not linked to the magnetic beads retains the adsorbed magnetic components, and is repeatedly washed 3 times with Tris buffer at pH = 7.5 for subsequent use.

[0042] (2) The...

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Abstract

The invention relates to a novel method utilizing specific functionalized nucleic acid sequence to realize biological logic operation and realizes complex logic circuit operation through series connection. The method comprises steps that the functionalized nucleic acid sequence is taken as basis, through the chain replacement and magnetic separation technology, signal input and operation and output signal chain separation are realized, a G-tetrad structure formed through free signal chains under a certain experiment condition is utilized to realize catalysis of oxidation reaction of ABTS-H2O2, ABTS+ having different wavelength absorptions is generated, through detection analysis on UV absorption conditions before and after, true or false of output signals can be acquired for binary system 0 or 1 during calculation in a computer. The method is advantaged in that an optimal reaction condition utilizing micro-RNA from a cell as an input signal is further explored, butt joint with reality cell application can be better realized, and high operation efficiency, strong interference immunity and wide application prospects are realized.

Description

technical field [0001] The invention relates to the research field of computational biology, in particular to a method for constructing biological logic gates and logic circuits based on nucleic acid. Background technique [0002] Computational Biology is a branch of biology. According to the definition of the National Institutes of Health (NIH), it refers to the development and application of data analysis and theoretical methods, mathematical modeling and computer simulation techniques for the study of biology, behavior and social group systems. . Alan Mathison Turing (Alan Mathison Turing, 1912-1957), the famous British mathematician and logician and the founder of computer logic, once pointed out that the number of sunflower spirals is a Fibonacci number, and the formation pattern of animal skin It can also be described as a reaction diffusion model (reaction diffusion model), when Turing lacked the data and computing power to build this model. With the development of...

Claims

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Application Information

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IPC IPC(8): G06N3/12
CPCG06N3/123
Inventor 纪小婷吕浩源马明辉吕炳霖丁彩凤
Owner QINGDAO UNIV OF SCI & TECH
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