Application of pleurotus ferulae polysaccharide in preparation of dog zona pellucida 3DNA vaccine adjuvant

A vaccine adjuvant, the technology of Asafoetida mushroom, applied in the directions of DNA/RNA vaccination, vaccine, intact cell/virus/DNA/RNA components, etc., can solve the problem of effective control of the number of stray dogs, high dosage of vaccine antigen, and poor immune effect. It is easy to control the quality, the adjuvant effect is obvious, and the immunogenicity is improved.

Active Publication Date: 2017-09-15
XINJIANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the problem that the existing canine zona pellucida 3DNA vaccine has low antigenicity, poor immune effect during use, and high dosage of vaccine antigen, which cannot effectively control the number of stray dogs, th

Method used

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  • Application of pleurotus ferulae polysaccharide in preparation of dog zona pellucida 3DNA vaccine adjuvant
  • Application of pleurotus ferulae polysaccharide in preparation of dog zona pellucida 3DNA vaccine adjuvant

Examples

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Example Embodiment

[0020] Example one:

[0021] The application of ferula polysaccharide in the preparation of canine zona pellucida 3 DNA vaccine adjuvant, wherein the mass ratio of ferula polysaccharide to canine zona zona 3 DNA vaccine is 10:1.

Example Embodiment

[0022] Embodiment two:

[0023] The application of ferula polysaccharide in the preparation of canine zona pellucida 3 DNA vaccine adjuvant, wherein the mass ratio of ferula polysaccharide to canine zona zona 3 DNA vaccine is 1:1.

Example Embodiment

[0024] Embodiment three:

[0025] The application of ferula polysaccharide in the preparation of canine zona pellucida 3 DNA vaccine adjuvant, wherein the mass ratio of ferula polysaccharide to canine zona pellucida 3 DNA vaccine is 2:1.

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Abstract

The invention discloses an application of pleurotus ferulae polysaccharide in preparation of a dog zona pellucida 3DNA vaccine adjuvant. According to the application, the purity range of the pleurotus ferulae polysaccharide is 40-95%, the mass ratio of the pleurotus ferulae polysaccharide to a dog zona pellucida 3DNA vaccine is (1:1) to (10:1), and the applicable immune animal is any one of a dog, a mouse and a rat. According to the provided pleurotus ferulae polysaccharide as the dog zona pellucida 3DNA vaccine adjuvant, when an effect verification experiment is carried out by using the rat, the IgG antibody level is obviously improved in comparison with that of an independent pcDNA3-CZP3 group, the total number born is reduced to 13 in comparison with 41 of the independent pcDNA3-CZP3 group after pleurotus ferulae polysaccharide combination, the pleurotus ferulae polysaccharide has good validity, the immunogenicity of an antigen can be better improved, and the immune effect can be strengthened.

Description

technical field [0001] The present invention relates to the technical field of medicine application, in particular, the present invention relates to the application of polysaccharide of mushroom asafoetida in vaccine adjuvant. Background technique [0002] Due to the uncontrollable birth control of stray dogs, the number of stray dogs in many big cities has increased sharply. Injury to people, killing livestock and spreading diseases have occurred from time to time. The killing method is relatively cruel, which is very likely to cause dissatisfaction among dog lovers and pressure from public opinion. Using canine ZP3 (Caninezona pellucida 3, CZP3) as the target antigen, the canine ZP3 DNA vaccine pcDNA3-CZP3 was developed, which not only has a certain anti-fertility effect, but also has broad application prospects. However, the vaccine has low antigenicity, poor immune effect during use, and high vaccine antigen dosage, which cannot effectively control the number of stray d...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/00A61P15/18
CPCA61K39/0006A61K39/39A61K2039/53A61K2039/552A61K2039/55583
Inventor 张富春林君媛李金玉王颖
Owner XINJIANG UNIVERSITY
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