Application of pleurotus ferulae polysaccharide in preparation of dog zona pellucida 3DNA vaccine adjuvant

Abstract

The invention discloses an application of pleurotus ferulae polysaccharide in preparation of a dog zona pellucida 3DNA vaccine adjuvant. According to the application, the purity range of the pleurotus ferulae polysaccharide is 40-95%, the mass ratio of the pleurotus ferulae polysaccharide to a dog zona pellucida 3DNA vaccine is (1:1) to (10:1), and the applicable immune animal is any one of a dog, a mouse and a rat. According to the provided pleurotus ferulae polysaccharide as the dog zona pellucida 3DNA vaccine adjuvant, when an effect verification experiment is carried out by using the rat, the IgG antibody level is obviously improved in comparison with that of an independent pcDNA3-CZP3 group, the total number born is reduced to 13 in comparison with 41 of the independent pcDNA3-CZP3 group after pleurotus ferulae polysaccharide combination, the pleurotus ferulae polysaccharide has good validity, the immunogenicity of an antigen can be better improved, and the immune effect can be strengthened.

Description

technical field [0001] The present invention relates to the technical field of medicine application, in particular, the present invention relates to the application of polysaccharide of mushroom asafoetida in vaccine adjuvant. Background technique [0002] Due to the uncontrollable birth control of stray dogs, the number of stray dogs in many big cities has increased sharply. Injury to people, killing livestock and spreading diseases have occurred from time to time. The killing method is relatively cruel, which is very likely to cause dissatisfaction among dog lovers and pressure from public opinion. Using canine ZP3 (Caninezona pellucida 3, CZP3) as the target antigen, the canine ZP3 DNA vaccine pcDNA3-CZP3 was developed, which not only has a certain anti-fertility effect, but also has broad application prospects. However, the vaccine has low antigenicity, poor immune effect during use, and high vaccine antigen dosage, which cannot effectively control the number of stray d...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the existing canine zona pellucida 3DNA vaccine has low antigenicity, poor immune effect during use, and high dosage of vaccine antigen, which cannot effectively control the number of stray dogs, the present invention Provide the application of ferulae polysaccharide in the preparation of canine zona pellucida 3DNA vaccine adjuvant, which can effectively improve the immune effect of the vaccine and reduce the dosage of vaccine antigen

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