Novel fluorescent probe as well as preparation method and application thereof in detecting uric acid

A fluorescent probe, a new type of technology, applied in the field of analytical chemistry, can solve problems such as the death of pregnant women, and achieve the effect of low cost

Inactive Publication Date: 2017-09-15
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

What is even more frightening is that some recent reports have confirmed that hyperuricemia is as

Method used

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  • Novel fluorescent probe as well as preparation method and application thereof in detecting uric acid
  • Novel fluorescent probe as well as preparation method and application thereof in detecting uric acid
  • Novel fluorescent probe as well as preparation method and application thereof in detecting uric acid

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Embodiment 1

[0028] Embodiment 1 novel fluorescent probe Tb III -dtpa-bis(2,6-Diaminopurine)

[0029] (1) Preparation method

[0030] 1, the preparation of diethylenetriaminepentaacetic dianhydride (dtpaa)

[0031] Weigh 7.8100g (0.02mmol) of dtpa, 16.0mL (0.08mmol) of acetic anhydride, and 10.0mL (0.12mmol) of pyridine into a three-necked round bottom flask, mix well, and heat with stirring at 65°C for 24h. Cool to room temperature, filter the reaction mixture, wash twice with a small amount of acetic anhydride and anhydrous ether, filter with a vacuum pump, and dry the resultant in a vacuum oven at 80°C to obtain dtpaa.

[0032] 2. Preparation of diethylenetriaminepentaacetic acid bis-2,6-diaminopurine (dtpa-bis(2,6-Diaminopurine))

[0033] Take 1.9610g of dtpaa (5.5mmol), 8.0mL (16.5mmol) of triethylamine, anhydrous DMF (50mL), and 1.6515g (11mmol) of 2,6-diaminopurine in a three-necked round bottom flask, and mix well. Heating at a constant temperature of 100°C and stirring rapidly...

Embodiment 2

[0039] Embodiment 2 fluorescent probe Tb III Application of -dtpa-bis(2,6-Diaminopurine) in detection of uric acid

[0040] 1. Fluorescence spectrum of fluorescent probe for detection of uric acid

[0041] Experimental conditions: Take a certain amount of uric acid (UV) and prepare it with Tris-HCl buffer solution with pH=7.4 to a concentration of 5.0×10 -4 mol / L solution as a uric acid stock solution.

[0042] Take 5.0 mL of uric acid stock solution in a 50 mL volumetric flask, add 5.0 mL of fluorescent probe stock solution prepared in Example 1, and use Tris-HCl buffer solution with pH=7.4 to make up volume. At this time, the concentration of the probe and each detection substance is 5.0×10 -5 mol / L. Changes in the fluorescence spectrum were observed at an excitation wavelength of 241 nm.

[0043] The result is as Figure 4a , Figure 4b shown. Such as Figure 4a As shown, under the excitation of 241nm, the fluorescent probe has a strong emission peak at 350nm. Uri...

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Abstract

The invention discloses a novel fluorescent probe, its preparation method and its application in detecting uric acid. Take diethylenetriaminepentaacetic acid, acetic anhydride, and pyridine, stir and heat at 65°C for 24 hours, cool, filter, wash, and dry, and the obtained diethylenetriaminepentaacetic dianhydride and triethylamine, DMF, 2,6‑ Diaminopurine, mix well, heat and stir at a constant temperature of 100°C for 24h, cool, filter, and dry, and the obtained diethylenetriaminepentaacetic acid-bis(2,6-diaminopurine) is dissolved in Tris-HCl buffer solution with pH=7.4 , mixed with Tb(NO3)3·6H2O, filtered, and the filtrate was heated or placed for a long time to obtain the target product. TbШ‑dtpa‑bis (2,6‑Diaminopurine) was used as a probe combined with a fluorescent method to detect uric acid. The method of the invention is simple and novel, has high efficiency and low cost, and can be applied in actual urine samples.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, in particular to the synthesis of novel fluorescent probes and the detection of uric acid. Background technique [0002] Uric acid is the final metabolite of purine in the human body, and the detection of uric acid content in human urine and blood is a very important indicator. Generally speaking, the normal concentration of uric acid in human urine should be 1.49-4.46mmol / L, and it maintains its own balance through various biological processes. When the production and excretion of uric acid are unbalanced, the oxidation product of uric acid, purine base, will be metabolically disturbed, resulting in hyperuricemia, gout, Lesch-Nyhan syndrome, such as kidney damage, leukemia, hypertension, diabetes and Hyperuricemia and so on. Leukemia patients can excrete up to 12g of uric acid within 1 day, while normal people only excrete about 600mg of uric acid in 1 day. What is even more frightening is...

Claims

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Application Information

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IPC IPC(8): C07F5/00C09K11/06G01N21/64
CPCC07F5/003C09K11/06C09K2211/182G01N21/6428G01N2021/6432
Inventor 王君杨帆刘逸伦张朝红宋有涛任佩佩刘冠宏
Owner LIAONING UNIVERSITY
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