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Extraction technology of snakegourd peel polysaccharides

An extraction process, the technology of Trichosanthes peel, which is applied in the field of polysaccharides extraction of Trichosanthes peel, can solve the problems of cost increase, yield disclosure, control of unfavorable product profits, etc., achieve high yield, high purity, and improve planting enthusiasm Effect

Inactive Publication Date: 2017-09-15
ANHUI JIUDIAN AGRI PROD DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, a kind of extraction process of Trichosanthes rind polysaccharide is given. The shortcoming of this extraction process is that the purity is relatively high, but the yield is revealed, only less than 30%, which causes the cost of Trichosanthes rind polysaccharide processing. Greatly improved, not conducive to the control of product profits

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] ①. After washing and drying the melon peel, it is ground under vacuum, and then sieved through a 100-mesh sieve to obtain melon peel powder;

[0019] ②. According to the material-to-liquid ratio of 1:40, mix the melon peel powder and water, then put it into pulse plasma for 20 minutes, then add pectinase, heat up to 35°C, stir and react for 6 hours;

[0020] ③. Centrifuge the reaction liquid at rest, take the liquid and concentrate it in vacuum to obtain the liquid extract;

[0021] ④. Add 75% ethanol to the liquid extract, heat and reflux for extraction, then cool, stand, filter, and retain the centrifuge; the filtrate is again concentrated in vacuum to obtain the liquid extract;

[0022] ⑤. Add 95% ethanol to the liquid extract obtained in step ④, heat again to reflux for extraction, then cool, stand, filter, and discard the filtrate;

[0023] ⑥ The centrifugal substance obtained in steps ④ and ⑤ is used to remove protein components by the Sevage method, so as to obtain the pol...

Embodiment 2

[0025] ①. After washing and drying the melon peel, it is ground under vacuum, and then sieved through a 100-mesh sieve to obtain melon peel powder;

[0026] ②. According to the material-to-liquid ratio of 1:60, mix the melon peel powder and water, then put it into pulse plasma for 10 minutes, then add pectinase, heat up to 45°C, stir and react for 6 hours;

[0027] ③. Centrifuge the reaction liquid at rest, take the liquid and concentrate it in vacuum to obtain the liquid extract;

[0028] ④. Add 75% ethanol to the liquid extract, heat and reflux for extraction, then cool, stand, filter, and retain the centrifuge; the filtrate is again concentrated in vacuum to obtain the liquid extract;

[0029] ⑤. Add 95% ethanol to the liquid extract obtained in step ④, heat again to reflux for extraction, then cool, stand, filter, and discard the filtrate;

[0030] ⑥. The centrifugal substance obtained in steps ④ and ⑤ is used to remove protein components by the Sevage method, so as to obtain the po...

Embodiment 3

[0032] ①. After washing and drying the melon peel, it is ground under vacuum, and then sieved through a 100-mesh sieve to obtain melon peel powder;

[0033] ②. According to the material-to-liquid ratio of 1:50, mix the melon peel powder and water, then put it into pulse plasma for 15 minutes, then add pectinase, heat up to 40℃, stir and react for 4.5 hours;

[0034] ③. Centrifuge the reaction liquid at rest, take the liquid and concentrate it in vacuum to obtain the liquid extract;

[0035] ④. Add 75% ethanol to the liquid extract, heat and reflux for extraction, then cool, stand, filter, and retain the centrifuge; the filtrate is again concentrated in vacuum to obtain the liquid extract;

[0036] ⑤. Add 95% ethanol to the liquid extract obtained in step ④, heat again to reflux for extraction, then cool, stand, filter, and discard the filtrate;

[0037] ⑥ The centrifugal substance obtained in steps ④ and ⑤ is used to remove protein components by the Sevage method, so as to obtain the po...

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Abstract

The invention relates to the technical field of production and processing of Trichosanthes kirilowii, and concretely relates to an extraction technology of snakegourd peel polysaccharides. The technology comprises the following steps: cleaning and drying snakegourd peel, carrying out vacuum milling, and sieving the milled snakegourd peel; mixing the obtained snakegourd peel powder with water, processing the obtained mixture in pulse plasma, adding pectase, and heating, stirring and reacting the mixture and the pectase; standing and reacting the obtained reaction solution, taking the obtained liquid, and carrying out vacuum concentrating to obtain a fluid extract; adding ethanol to the fluid extract, carrying out heating refluxing extraction, carrying out cooling, standing and filtering, and reserving the centrifuged product; carrying out vacuum concentrating on the obtained filtrate to obtain a fluid extract; adding ethanol to the fluid extract, carrying out heating refluxing extraction, carrying out cooling, standing and filtering, and removing the obtained filtrate; and removing proteins from the above obtained centrifuged products through a Sevage technology in order to obtain the snakegourd peel polysaccharides. Technologic parameters are reasonably designed, so the extraction technology of snakegourd peel polysaccharides is simple and convenient, and the extracted snakegourd peel polysaccharides have high purity and very high yield, and the plantation positivity of the Trichosanthes kirilowii is greatly improved.

Description

Technical field [0001] The invention relates to the technical field of production and processing of Trichosanthes trichorum, in particular to an extraction process of polysaccharides from Trichosanthes trichorum skin. Background technique [0002] Gualou, also known as wild gourd, scientific name Gualou, contains calcium, magnesium, zinc, copper, protein, vitamins and other beneficial components to the human body. It has comprehensive effects such as lowering blood pressure, lowering blood sugar, and lowering blood lipids. It has high medicinal properties. Health value. [0003] Gualou peel is the peel of Gualou, the petals are boat-shaped, the edges are curled inside, the outer surface is orange-red or orange-yellow, shrunken, and some have residual stubs or stems, and the inner surface is yellow-white. It is brittle and easy to break. It is fragrant and sweet, sweet and slightly sour. [0004] Chinese patent application CN 106214694 A discloses an application of polysaccharides ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 陈娇
Owner ANHUI JIUDIAN AGRI PROD DEV CO LTD
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