Method and detecting tube for detecting soybean urease activity

A urease activity detection tube technology, applied in the field of enzyme activity detection, can solve the problems of simple and fast detection of soybean urease, difficulty in on-site detection, long detection time, etc., and achieve simple structure, stable sampling quality, and short time-consuming Effect

Active Publication Date: 2017-09-19
HENAN UNIVERSITY OF TECHNOLOGY
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  • Abstract
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Problems solved by technology

The results of these two methods are very accurate, but require certain equipment conditions and skilled operating techniques, and the detection time is long, so it

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  • Method and detecting tube for detecting soybean urease activity
  • Method and detecting tube for detecting soybean urease activity
  • Method and detecting tube for detecting soybean urease activity

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Embodiment Construction

[0025] The technical solutions of the present invention will be described in further detail below through specific implementation methods.

[0026] This embodiment provides a soybean urease activity detection tube, such as figure 1 , figure 2 and image 3 As shown, it includes urea cresol red sodium salt extract solution tube 1, sample quantitative tube 2 and bottle cap 3. The top opening of the urea cresol red sodium salt extraction solution tube 1 and the openings at both ends of the sample quantitative tube 2 . One end of the bottle cap 3 and the top of the urea cresol red sodium salt extract tube 1 are respectively provided with external threads 5 , and the two ends of the sample quantitative tube 2 are respectively provided with sealing rings 4 movably. The bottom of the sample quantitative tube 2 is screwed on the top of the urea cresol red sodium salt extract tube 1, and the bottle cap 3 is screwed on the top of the sample quantitative tube 2, wherein the capacity o...

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Abstract

The invention provides a soybean urease activity detecting tube which comprises a urea cresol red sodium salt extracting fluid tube, a sample quantifying tube and a bottle cap which are sequentially connected. The detecting tube has simple structure and stable sampling quality and avoids large fluctuation. The invention further provides a method for detecting soybean urease activity. The method comprises the steps of adding a soybean meal sample into the sample quantifying tube, connecting the sample quantifying tube with the top of the urea cresol red sodium salt extracting fluid tube, shocking, recording color change time and calculating a soybean urease activity value of the soybean meal sample according to a corresponding fitted equation. The detecting method has simple steps, short consumed time in a detecting process and not high requirements for skilled degree of operators and equipment; furthermore, detecting results are identical with detection results of a national-standard method.

Description

technical field [0001] The invention belongs to a method for detecting enzyme activity, in particular to a method for detecting soybean urease activity and a detection tube. Background technique [0002] The trypsin inhibitor contained in soybeans restricts the application of soybean products. After eating by animals, it is easy to cause symptoms such as loose stools or soft stools, especially for piglets, which is more likely to cause diarrhea in piglets. Because trypsin inhibitor is difficult to detect, and the properties of urease in soybean are similar to that of trypsin inhibitor, the method of measuring urease activity is usually used to determine the degree of rawness and maturity of soybean. The methods for the quantitative determination of urease activity in the laboratory are relatively complicated, including titration and pH value-increasing methods. The results of these two methods are very accurate, but require certain equipment conditions and skilled operating...

Claims

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Application Information

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IPC IPC(8): C12Q1/34
CPCC12Q1/34
Inventor 王金荣苏兰利李硕赵银丽安娜
Owner HENAN UNIVERSITY OF TECHNOLOGY
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