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Tissue culture and rapid reproduction method of bletilla striata

The technology of Bletilla striata group and Bletilla striata is applied in the directions of horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of large bulbs, improved seed germination rate, and fast germination speed.

Inactive Publication Date: 2017-09-22
HEFEI BAILYVSHENG AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing Bletilla striata tissue culture rapid propagation technology needs to be further improved in terms of seed germination rate, transplanting survival rate, tissue culture seedling rooting and bulb size, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A bletilla striata tissue culture rapid propagation method is carried out according to the following steps:

[0025] (1) Disinfection of explants: Select healthy and disease-free Bletilla striata seeds as explants, wash off the hair and foreign matter on the surface of the seeds with water, then transfer to the ultra-clean workbench, wash twice with sterile water, put Soak in 75% alcohol for 30 seconds, then wash twice with sterile water, and then sterilize in 0.1% mercuric solution for 5 minutes. During this period, keep shaking to make it thoroughly sterilized and sterile after disinfection. Wash with water 4 times, and finally blot the surface moisture with sterile filter paper for later use;

[0026] (2) Inoculation induction: inoculate the explants after the disinfection of step (1) on the solid medium for callus induction, and promote germination into seedlings. The solid medium is composed of two basic types of LS medium and WPM medium. The culture medium is com...

Embodiment 2

[0037] A bletilla striata tissue culture rapid propagation method is carried out according to the following steps:

[0038] (1) Disinfection of explants: Select healthy and disease-free Bletilla striata seeds as explants, wash off the hair and foreign matter on the surface of the seeds with water, then transfer to the ultra-clean workbench, wash with sterile water 3 times, put Soak in 75% alcohol for 35 seconds, then wash with sterile water for 3 times, and then sterilize in 0.2% mercuric solution for 10 minutes. During this period, keep shaking to make it thoroughly sterilized and sterile after disinfection. Wash with water 5 times, and finally dry the surface moisture with sterile filter paper for later use;

[0039] (2) Inoculation induction: inoculate the explants after the disinfection of step (1) on the solid medium for callus induction, and promote germination into seedlings. The solid medium is composed of two basic types of LS medium and WPM medium. The culture mediu...

Embodiment 3

[0050] A bletilla striata tissue culture rapid propagation method is carried out according to the following steps:

[0051] (1) Disinfection of explants: Select healthy and disease-free Bletilla striata seeds as explants, wash off the hair and foreign matter on the surface of the seeds with water, then transfer to the ultra-clean workbench, wash 4 times with sterile water, put Soak in 75% alcohol for 40 seconds, wash with sterile water for 4 times, and then sterilize in 0.3% mercuric solution for 15 minutes. During this period, keep shaking to make it thoroughly sterilized and sterile after disinfection. Wash with water for 6 times, and finally dry the surface moisture with sterile filter paper for later use;

[0052] (2) Inoculation induction: inoculate the explants after the disinfection of step (1) on the solid medium for callus induction, and promote germination into seedlings. The solid medium is composed of two basic types of LS medium and WPM medium. The culture medium...

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PUM

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Abstract

The invention discloses a tissue culture and rapid reproduction method of bletilla striata, and relates to the field of tissue-culture reproduction of flowers. The method comprises the following steps of explant disinfection, wherein healthy bletilla striata seeds without diseases are selected as explants and are subjected to disinfection treatment; 2, inoculation induction, wherein the explants are inoculated on solid culture media and are subjected to callus induction, so that germinating and seedling are promoted; 3, proliferation and subculture, wherein seedling stems are cut and transferred into the solid subculture media until the stems are proliferated, and cluster buds are obtained; 4, rooting culture. The issue culture and rapid reproduction method is simple and easy to operate, the germination rate of the seeds can be increased, the culture time is shortened, the bulb size of the obtained tissue-culture seedlings is increased, the rooting rate and the transplanting survival rate are increased, so that the tissue-culture seedlings of bletilla striata are conveniently and rapidly reproduced, and the method meets market requirements and is suitable for popularization and application.

Description

technical field [0001] The invention relates to the field of tissue culture and breeding of flowers, in particular to a method for tissue culture and rapid propagation of bletilla striata. Background technique [0002] Bletilla striata, also known as Lianjicao, Gangen, Baigi, Ruolan, Zhulan, Zilan, Zihui, and Baili, is a perennial herbaceous plant of the genus Bletilla striata in the family Orchidaceae. It is widely distributed in the provinces of the Yangtze River Basin in my country, mainly produced in Guizhou, Sichuan, Hunan, Hubei, Anhui, Henan, Zhejiang, Shaanxi and other places. Bletilla striata not only has high ornamental value, but also one of the important Chinese medicinal materials in my country. The tuber is used as medicine, which can astringe and stop bleeding, reduce swelling and promote muscle growth. It is widely used in the treatment of pulmonary tuberculosis, bronchiectasis, hemoptysis, and gastric ulcer bleeding. [0003] For a long time, the bletilla st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨自芬
Owner HEFEI BAILYVSHENG AGRI TECH CO LTD
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