Method for determining eugenol in aquatic products using isotope dilution gas chromatography mass spectrometry

Abstract

The invention discloses a method for determining eugenol in aquatic products using isotope dilution gas chromatography mass spectrometry, and relates to the technical field of physicochemical detection of drug residues in the aquatic products. A muscle sample is extracted by an organic solution in advance, a C18 solid phase extraction column is adopted to conduct purification, GC-MS / MS MS analysis is adopted to determine the drug residues in the aquatic products, the method comprises the following steps that preparation is conducted, wherein fish body dissection and homogenization are conducted, and low-temperature storage is conducted in a refrigerator at 20 DEG C; isotopes are added for internal standard and mixing is conducted uniformly; the organic solution is adopted for extraction; the C18 solid phase extraction column is adopted to conduct the purification; redissolving is conducted after rotary evaporation and concentration; supernatant is absorbed to be subjected to the GC-MS / MS MS analysis after being filtered by an organic phase filtration membrane of 0.22 micrometer. By the adoption of the method for determining eugenol in the aquatic products using the isotope dilution gas chromatography mass spectrometry, the defects are overcome that an existing external standard quantitative method is strong in substrate interference and poor in quantitative accuracy, and the method has the advantages of being less in interference and high in accuracy and sensitivity.

Description

Technical field [0001] The invention relates to the technical field of physical and chemical detection of drug residues in aquatic products, in particular to a method for determining eugenol in aquatic products by isotope dilution gas chromatography mass spectrometry. Background technique [0002] Aquaculture is a traditional and important industry in my country. It has developed rapidly in recent years and has become the main source of my country's aquatic product supply, which has greatly enriched and met the consumption needs of the people. In order to improve the survival rate of fresh aquatic products and reduce economic losses, anesthetics for fishery are used in aquatic product breeding, transportation and surgery. Eugenol is one of the most widely used anesthetics in domestic aquatic products due to its good efficacy and low price. Regarding the use of eugenol anesthetics in the aquatic industry, the relevant management research in my country is still in a fuzzy area, an...

AI Technical Summary

Problems solved by technology

In the previous work, we have established an external standard method for the determination of eugenol residues in fish by gas chromatography-tandem mass spectrometry (GC-MS / MS). However, due to the high content of interfering substances such as fat and protein in aquatic samples, the external standard The matrix interference is strong during the quantitative process, which limits the accuracy of quantitative determination