Method for preparing GABA (gamma-aminobutyric acid) tea through microbial fermentation

A technology of microbial fermentation and lactic acid bacteria fermentation, applied in the field of microbial fermentation to produce GABA tea, can solve the problems of not being popularized and applied, damage to tea quality, difficult operating conditions, etc., to achieve long-term preservation and storage, increase utilization rate, and uniform quality Effect

Active Publication Date: 2017-09-26
四会市桔田源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the operating conditions of the above methods are difficult to control, such as infrared and microwave irradiation are easy to leak

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0051] Example 1:

[0052] A method for producing GABA tea by microbial fermentation includes the following steps:

[0053] (1) Preparation of lactic acid bacteria fermentation seed liquid: Lactobacillus brevis (Lactobacillus brevis) AS1.214 was cultured for 12 hours, the bacteria were collected after centrifugation, and sterilized water was used to prepare lactic acid bacteria fermentation seed liquid for later use.

[0054] (2) Fresh leaf picking: picking one bud, three and four leaves of tea trees;

[0055] (3) Finishing: use a microwave oven for 2 minutes;

[0056] (4) Kneading: Use a kneading machine to knead the green leaves cooled to 30~35℃ for 20 minutes;

[0057] (5) Sterilization: Sterilize the kneaded fresh leaves in a high-pressure steam sterilizer at 121°C for 15 minutes.

[0058] (6) Inoculation: The sterilized tea leaves are soaked in sterile water at a mass ratio of 20%, and the lactic acid bacteria fermentation seed liquid obtained in step (1) is added to the inoculum amo...

Example Embodiment

[0062] Example 2

[0063] A method for producing GABA tea by microbial fermentation includes the following steps:

[0064] (1) Preparation of lactic acid bacteria fermentation seed liquid: Streptococcus thermophiles AS1.6472 was cultured for 14 hours, the bacteria were collected after centrifugation, and sterilized water was used to prepare lactic acid bacteria fermentation seed liquid for later use.

[0065] (2) Fresh leaf picking: picking one bud, three and four leaves of tea trees;

[0066] (3) Finishing: use a microwave oven for 2.5min;

[0067] (4) Kneading: Use a kneading machine to knead the frozen leaves cooled to 30~35℃ for 15min;

[0068] (5) Sterilization: Sterilize the kneaded fresh leaves in a high-pressure steam sterilizer at 121°C for 15 minutes.

[0069] (6) Inoculation: The sterilized tea leaves are soaked in sterile water at a mass ratio of 30%, and the lactic acid bacteria fermentation seed liquid obtained in step (1) is added to the inoculum of 1%.

[0070] (7) Fermenta...

Example Embodiment

[0073] Example 3

[0074] A method for producing GABA tea by microbial fermentation includes the following steps:

[0075] (1) Preparation of lactic acid bacteria fermentation seed liquid: Lactobacillus plantarum subsp. plantarum AS1.3919 was cultured for 16 hours, and the cells were collected after centrifugation, and the lactic acid bacteria fermentation seed liquid was prepared with sterile water for later use.

[0076] (2) Fresh leaf picking: picking one bud, three and four leaves of tea trees;

[0077] (3) Finishing: Use a microwave oven to finish for 3 minutes;

[0078] (4) Kneading: Use a kneading machine to knead the green leaves cooled to 30~35℃ for 20 minutes;

[0079] (5) Sterilization: Sterilize the kneaded fresh leaves in a high-pressure steam sterilizer at 121°C for 15 minutes.

[0080] (6) Inoculation: The sterilized tea leaves are soaked in sterile water at a mass ratio of 20%, and the lactic acid bacteria fermentation seed liquid obtained in step (1) is added to the inocu...

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Abstract

The invention discloses a method for preparing GABA (gamma-aminobutyric acid) tea through microbial fermentation, and belongs to the technical field of tea processing. The method comprises the following steps: performing microwave deactivation on picked fresh tea leaves, rolling, performing high-temperature sterilization, soaking 20 to 60 percent by mass of tea into sterile water, adding a lactobacillus fermentation seed liquid under the conditions that the inoculation amount is 1 to 6 percent, the fermentation temperature is 32 to 42 DEG C and the fermentation time is 24 to 48 hours, taking out the tea after fermentation and drying. By the method provided by the invention, mechanical injury is reduced, the tea can be prevented from being red and broken, and the quality of the finished product tea is uniform. The method is simple in operation and high in controllability. The GABA tea prepared by the method has sour, cool and faint scent, mellow and fresh flavor and light yellow soup, the appearance strips are uniform and tight, the color and luster are oily and dark green, the leaf bottom is uniform and neat, the leaf quality is soft, and the GABA content reaches gamma-aminobutyric acid tea standard; the antibacterial effect is achieved, and long-time preservation and storage are benefited.

Description

technical field [0001] The invention belongs to the technical field of tea processing, in particular to a method for preparing GABA tea by microbial fermentation. Background technique [0002] 1. Overview of γ-aminobutyric acid [0003] Protein is the material basis of life and the main bearer of life activities. Protein is formed by the arrangement and combination of amino acids in a certain way. More than 700 kinds of amino acids have been found, including natural amino acids and artificial amino acids. Most proteins in nature are composed of 20 amino acids in different conformations, and these amino acids are called protein amino acids. Non-protein amino acids refer to a class of substances that are similar in structure to protein amino acids and also contain amino and carboxyl groups, but do not form proteins. Some of these amino acids have special physiological activities, so they are called functional amino acids. γ-aminobutyric acid (GABA for short) is such a func...

Claims

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Application Information

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IPC IPC(8): A23F3/10
CPCA23F3/10
Inventor 黄亚辉陈莹玉张旭曾贞
Owner 四会市桔田源生物科技有限公司
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