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Schistosoma japonicum SjPPase recombinant antigen protein and application thereof in therapy evaluation

A technology of recombinant antigen protein and schistosomiasis, applied in the field of immunology, can solve the problems of reducing the sensitivity of schistosomiasis to drugs, increasing the mental burden of drug users, etc., and achieves the effects of high positive detection rate, broad market space and strong specificity.

Inactive Publication Date: 2017-09-29
STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Existing immunodiagnostic antigens cannot effectively distinguish current infection from past infection, so it often leads to repeated medication of cured patients, which not only increases the mental burden of drug users, but also may reduce the sensitivity of schistosomiasis to drugs

Method used

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  • Schistosoma japonicum SjPPase recombinant antigen protein and application thereof in therapy evaluation

Examples

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Effect test

Embodiment 1

[0014] Example 1 Evaluation of Schistosoma japonicum SjPPase Recombinant Antigen Protein for Diagnosis and Curative Effect Evaluation of Schistosomiasis japonicum

[0015] 1 material

[0016] 1.1 Serum samples of praziquantel patients with schistosomiasis before treatment, 3 months after treatment, and 6 months after treatment were collected from endemic areas of schistosomiasis in Sichuan Province; serum samples from normal people were collected from Suzhou City

[0017] 1.2 Goat anti-human whole molecule IgG, from SIGMA company.

[0018] 2 methods

[0019] 2.1 Recombinant antigen SjPPase detection results of sera of schistosomiasis patients and normal people before praziquantel treatment

[0020] 1) Dilute the antigen with coating solution to a final concentration of 4ng / μl, 100μl / well, incubate at 37°C for 2h, wash with PBS-T 3 times, each time for 3min;

[0021] 2) Use 1% BSA (diluted in PBS), 100 μl / well, incubate at 37°C for 1 hour, wash with PBS-T 3 times, 3 minutes ...

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Abstract

The invention discloses an application of a schistosoma japonicum (SjPPase) recombinant antigen protein in therapy evaluation of the schistosoma japonicum and belongs to the field of immunology. An amino acid sequence of the schistosoma japonicum (SjPPase) recombinant antigen protein is as shown in SEQ ID NO:1, and the schistosoma japonicum (SjPPase) recombinant antigen protein is the protein capable of catalyzing hydrolysis of inorganic pyrophosphate (PPi) to generate phosphate. The application of the SjPPase recombinant protein in diagnosis of the schistosoma japonicum is provided for the first time, and the SjPPase recombinant protein can be applied to diagnosis of the schistosoma japonicum, is capable of carrying out therapy evaluation, and has a wide market space and relatively good social and economic benefits.

Description

technical field [0001] The invention belongs to the field of immunology, and in particular relates to the application of a recombinant antigenic protein of schistosomiasis inorganic pyrophosphatase (SjPPase) in the curative effect assessment of schistosomiasis japonicum. Background technique [0002] Schistosomiasis japonicum is a zoonotic parasitic disease caused by humans or mammals infected with Schistosoma japonicum, mainly distributed in southern China, the Philippines and Indonesia. Although there have been more than 50 years of schistosomiasis prevention and control history in China, schistosomiasis is still one of the most important public health problems in China. It is mainly prevalent in 110 counties and urban areas in 7 provinces of my country. There are still 790,000 patients and the population is at risk. 65 million. [0003] The developmental stages from juveniles to adults of Schistosoma japonicum are completed in mammalian hosts. During this process, juvenil...

Claims

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Application Information

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IPC IPC(8): C12N9/14G01N33/573
CPCC12N9/14C12Y306/01001G01N33/573G01N2333/914
Inventor 陈绅波陈军虎胡薇徐斌
Owner STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
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